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pRS415-CUP1pr

Plasmid

#221106

Purpose

Copper-inducible gene expression

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Type

Empty backbone

Expression

Yeast

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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POSV801-SP44-SnaAinBCDEOT1T2

Plasmid

#225056

Purpose

Heterologous expression in Streptomyces with inactive SnaA

Depositor

Wilfred van der Donk

Article

Yu et al ACS Cent Sci. 2024 Jun 3;10(6):1242-1250

Insert

inactivated SnaA

Expression

Bacterial

Mutation

S556A/S1616A

Promoter

SP44

Available Since

March 5, 2025

Availability

Academic Institutions and Nonprofits only

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POSV801-SP44-SnaABCDEinOT1T2

Plasmid

#225057

Purpose

Heterologous expression in Streptomyces with inactive SnaE

Depositor

Wilfred van der Donk

Article

Yu et al ACS Cent Sci. 2024 Jun 3;10(6):1242-1250

Insert

inactivated SnaE

Expression

Bacterial

Mutation

deltaA46-V801

Promoter

SP44

Available Since

March 5, 2025

Availability

Academic Institutions and Nonprofits only

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pHW622

Plasmid

#229868

Purpose

Entry vector with reverse FRT3 (rFRT3) in slot 7 (for SapTrap assembly)

Depositor

Han Wang

Article

Khan et al PLoS Genet. 2025 Jan 22;21(1):e1011541.

Insert

reverse FRT3 (rFRT3)

Expression

Worm

Available Since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Med15

Plasmid

#231887

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 Med15

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Med15

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 R3 W

Plasmid

#231876

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 R3 W

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 R3 W

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

D103R, N126W, D133R, K143R

Promoter

T7

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 R2

Plasmid

#231870

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 R2

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 R2

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

K118R, K140R

Promoter

T7

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 L123A+

Plasmid

#231869

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 L123A+

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 L123A+

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

L123A

Promoter

T7

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 Swap2

Plasmid

#231865

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 Swap2

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 Swap2

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 F124I

Plasmid

#231864

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 F124I

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

F124I

Promoter

T7

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 Swap3

Plasmid

#231860

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 Swap3

Depositor

Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 Swap3

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 WT 44mer

Plasmid

#231856

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 WT 44mer

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 44mer

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_FL Gcn4

Plasmid

#231855

Purpose

Bacterial expression of N-terminally 6His tagged FL Gcn4

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pSuper TR G414C

Plasmid

#207612

Purpose

U3 promoter driven expression of the telomerase RNA with a G414C CAB box mutation

Depositor

Jens Schmidt

Article

Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

Insert

Telomerase RNA G414C

Expression

Mammalian

Promoter

U1

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAM198

Plasmid

#227657

Purpose

pRS305 His-HRV3C-Rpn5 WT

Depositor

Andreas Martin

Article

Greene et al Elife. 2019 Nov 28;8:e49806. doi: 10.755

Insert

rpn5 (RPN5 Budding Yeast)

Tags

His-HRV3C-

Expression

Yeast

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAM200

Plasmid

#227659

Purpose

pRS305 3XFLAG-HRV3C-Rpn5 WT

Depositor

Andreas Martin

Article

Greene et al Elife. 2019 Nov 28;8:e49806. doi: 10.755

Insert

rpn5 (RPN5 Budding Yeast)

Tags

3X FLAG-HRV3C-

Expression

Yeast

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAM202

Plasmid

#227661

Purpose

pRS316 Rpn6 promoter-Rpn5 ORF-Rpn5 terminator

Depositor

Andreas Martin

Article

Greene et al Elife. 2019 Nov 28;8:e49806. doi: 10.755

Insert

rpn5 (RPN5 Budding Yeast)

Expression

Yeast

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAM248

Plasmid

#226892

Purpose

6xHis-PSP-GS-PANN(75-150)-(5aaGS)-Msp1(36-362)_W166A/Y167A

Depositor

Andreas Martin

Article

Castanzo et al Proc Natl Acad Sci U S A. 2020 Jun 30;11

Insert

msp1 (MSP1 Budding Yeast)

Tags

His-Precission-PANN

Expression

Bacterial

Mutation

W166A/Y167A

Promoter

T7

Available Since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pAM211 His-Ubp6

Plasmid

#226359

Purpose

Expression of yeast Ubp6

Depositor

Andreas Martin

Article

Bashore et al Nat Struct Mol Biol. 2015 Sep;22(9):712-

Insert

ubp6 (UBP6 Budding Yeast)

Tags

His

Expression

Bacterial

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pAM257

Plasmid

#226901

Purpose

6xHis-PSP-MBP-Pex3(40-441)

Depositor

Andreas Martin

Article

Castanzo et al Proc Natl Acad Sci U S A. 2020 Jun 30;11

Insert

pex3 (PEX3 Budding Yeast)

Tags

6xHis-PSP-MBP

Expression

Bacterial

Mutation

amino acids 40-441

Promoter

T7

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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