We narrowed to 11,499 results for: ARIA

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hKCNB1 G381R

Plasmid

#124490

Purpose

missense mutation in children with EOEE. HA tagged in C-terminus, pCIneo vector

Depositor

Federico Sesti

Article

Yu et al FASEB J. 2019 Dec;33(12):14680-14689. do

Insert

human KCNB1 (KCNB1 Human)

Tags

HA

Expression

Mammalian

Mutation

changed glycine at 381 to arginine

Available Since

Sept. 3, 2019

Availability

Academic Institutions and Nonprofits only

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hKCNB1 F416L

Plasmid

#124491

Purpose

missense mutation in children with EOEE. HA tagged in C-terminus, pCIneo vector

Depositor

Federico Sesti

Article

Yu et al FASEB J. 2019 Dec;33(12):14680-14689. do

Insert

human KCNB1 (KCNB1 Human)

Tags

HA

Expression

Mammalian

Mutation

changed phenyalanine 416 to leucine

Available Since

Sept. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3-SREBP-1 (A317-318)

Plasmid

#204193

Purpose

Mammalian expression of SREBP-1 (isoform 1)

Depositor

Allan Weissman

Article

Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

Insert

SREBP-1 (SREBF1 Human)

Tags

E-tag and T7x2

Expression

Mammalian

Mutation

Gly to Ala 317, 318

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3-SREBP-1 (A317-318; A341)

Plasmid

#204194

Purpose

Mammalian expression of SREBP-1 (Isoform 1)

Depositor

Allan Weissman

Article

Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

Insert

SREBP-1 (SREBF1 Human)

Tags

E-tag and T7x2

Expression

Mammalian

Mutation

Gly to Ala 317,318,341

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-SpCas9-VRQR-P2A-EGFP (RTW3161)

Plasmid

#139992

Purpose

CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRQR(D1135V/G1218R/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Walton et al Science. 2020 Apr 17;368(6488):290-296.

Insert

human codon optimized SpCas9-VRQR with BPNLS-3xFLAG-P2A-EGFP

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

VRQR=D1135V/G1218R/R1335Q/T1337R

Promoter

CMV and T7

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-intron-hfCas13d-pA/EF1a-mCherry-WPRE-pa

Plasmid

#233034

Purpose

To Express HA tagged hfCas13d the CMV promoter. Also encodes a mCherry gene outside of the viral genome

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

hfCas13d and mCherry

Use

AAV

Tags

mCherry

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-CDH5-mRuby3-IRES-puro

Plasmid

#85144

Purpose

lentiviral expression of human VE-cadherin, C-terminally tagged with mRuby3

Depositor

Tobias Meyer

Article

Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

Insert

CDH5-mRuby3 (VE-cadherin, C-terminally tagged with mRuby3 (CDH5 Human)

Use

Lentiviral

Tags

mRuby3

Promoter

CAG

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pPGK-T7/2-CD44v3 (fl)

Plasmid

#137814

Purpose

expression of CD44 proteins in mammalian cells

Depositor

Ursula Gunthert

Article

Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

Insert

CD44v3 (fl) (CD44 Human)

Tags

GFP

Expression

Mammalian

Mutation

full length and N280D in the V3 region

Promoter

PGK

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-intron-DjCas13d-pA/EF1a-mCherry-WPRE-pa

Plasmid

#233033

Purpose

To Express HA tagged DjCas13d the CMV promoter. Also encodes a mCherry gene outside of the viral genome

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

DjCas13d and mCherry

Use

AAV

Tags

mCherry

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-eNme2-T.2-ABE8e

Plasmid

#185669

Purpose

Expresses eNme2-T.2-ABE8e in mammalian cells

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

Insert

ecTadA(8e)-neNme2-T.2

Use

CRISPR

Expression

Mammalian

Mutation

Nme2Cas9 D16A/E47K/R63K/V68M/A116T/T123A/D152N/E1…

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1_RAF1 (51-131)

Plasmid

#119218

Purpose

purification of GST-RAF1 RBD from E. coli

Depositor

Helen Morrison

Article

Riecken et al Hum Mutat. 2015 Feb;36(2):270-8. doi: 10

Insert

RAF1 aa 51-131 (RAF1 Human)

Expression

Bacterial

Available Since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pPGK-T7/2-CD44s (-cyt)

Plasmid

#137815

Purpose

expression of CD44 proteins in mammalian cells

Depositor

Ursula Gunthert

Article

Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

Insert

CD44s (-cyt) (CD44 Human)

Tags

GFP

Expression

Mammalian

Mutation

without cytoplasmic region

Promoter

PGK

Available Since

June 19, 2020

Availability

Academic Institutions and Nonprofits only

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GB1-A11(2-196, D40G)-Strep

Plasmid

#196210

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196, D40G)-Strep.

Depositor

Lalit Deshmukh

Article

Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

Insert

Annexin A11 (ANXA11 Human)

Tags

GB1-6xHis-TEV and Strep-II tag

Expression

Bacterial

Mutation

D40G

Available Since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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2xMARS

Plasmid

#205233

Purpose

Expresses two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to mScarlet-iin mammalian cells

Depositor

Jeremy Baskin

Article

Cao et al Nat Cell Biol. 2024 Oct;26(10):1804-1816

Insert

two repeats of PLEKHA5 aa 143-271 with K163A and R164A mutations (PLEKHA5 Human)

Tags

Nuclear Export Sequence and mScarlet-i

Expression

Mammalian

Mutation

amino acids 143-271 of PLEKHA5 (GenBank reference…

Promoter

CMV

Available Since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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pETM6-G6-vioABE

Plasmid

#73438

Purpose

Prodeoxyviolacein pathway (vioABE) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variant G6.

Depositor

Mattheos Koffas

Article

Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

Insert

PG6-vioABE

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

PG6 (orthogonal T7-lac variant)

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.SF-iGluSnFR.A184S

Plasmid

#106198

Purpose

Tight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-iGluSnFR.A184S

Use

AAV

Mutation

GltI: A184S

Promoter

CAG

Available Since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pT7-G-AsCas12a crRNA-BsaI cassette (MSP3491)

Plasmid

#114067

Purpose

T7 promoter crRNA entry vector used for in vitro transcription of AsCas12a guides

Depositor

Keith Joung, Benjamin Kleinstiver

Article

Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

Insert

entry vector for AsCas12a crRNAs

Use

In vitro transcription

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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GB1-A11(2-196, G38R)-Strep

Plasmid

#196209

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196, G38R)-Strep.

Depositor

Lalit Deshmukh

Article

Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

Insert

Annexin A11 (ANXA11 Human)

Tags

GB1-6xHis-TEV and Strep-II tag

Expression

Bacterial

Mutation

G38R

Available Since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-CDH5-mCitrine-IRES-puro

Plasmid

#85143

Purpose

lentiviral expression of human VE-cadherin, C-terminally tagged with mCitrine

Depositor

Tobias Meyer

Article

Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

Insert

CDH5-mCitrine (VE-cadherin, C-terminally tagged with mCitrine (CDH5 Human)

Use

Lentiviral

Tags

mCitrine

Promoter

CAG

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-TAF4B

Plasmid

#192927

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of TAF4B

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

TAF4B (TAF4B Human)

Expression

Mammalian

Promoter

TET3G-miniCMV

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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