We narrowed to 9,938 results for: Pol

Showing: 7101 - 7120 of 9938 results

Results per page:

Sort results by:

pTWIN1-His6-Ssp-Httex1-29Q

Plasmid

#84350

Purpose

Expression of the human Huntingtin Exon1 protein containing 29Q in E.coli

Depositor

Hilal Lashuel

Article

Vieweg et al J Biol Chem. 2016 Jun 3;291(23):12074-86

Insert

Huntingtin Exon 1 (HTT Human)

Tags

N-terminal Ssp intein (His-tagged)

Expression

Bacterial

Promoter

T7

Available Since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pUDP044

Plasmid

#101168

Purpose

pUDP004 expressing a polycistronic g-RNA array for Cas9 editing targeting the genes SeATF1 and SeATF2 and Spcas9D147Y P411T in S. pastorianus (HH-gRNASeATF1-HDV-linker-HH-gRNASeATF2-HDV)

Depositor

Jean-Marc Daran

Article

de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.

Insert

polycistronic HH-gRNA-HDV-HH-gRNA-HDV array targetting SeATF1 and 2 in S. pastorianus

Use

CRISPR

Expression

Yeast

Promoter

ScTDH3

Available Since

Dec. 4, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pQCXIB Flag MFF WT

Plasmid

#74384

Purpose

Retroviral expression vector containing Flag tagged WT MFF isoform 5

Depositor

Reuben Shaw

Article

Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

Insert

Flag-MFF (MFF Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

May 10, 2016

Availability

Academic Institutions and Nonprofits only
pET28_6xHis-PARP1-A774L

Plasmid

#174796

Purpose

Bacterial expression of a hyperactive PARP1 mutant (A774L destabilizes the autoinhibitory HD subdomain)

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-A774L (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

A774L, V762A

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28_6xHis-PARP1-W318R

Plasmid

#174793

Purpose

Bacterial expression of an inactive PARP1 (W318R disrupts interdomain communication and HD subdomain unfolding)

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-W318R (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

W318, V762A

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pTWIN1-His6-Ssp-Httex1-15Q

Plasmid

#84348

Purpose

Expression of the human Huntingtin Exon1 protein containing 15Q in E.coli

Depositor

Hilal Lashuel

Article

Vieweg et al J Biol Chem. 2016 Jun 3;291(23):12074-86

Insert

Huntingtin Exon1 (HTT Human)

Tags

N-terminal Ssp intein (His-tagged)

Expression

Bacterial

Promoter

T7

Available Since

Dec. 19, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3 Flag MFF iso5 S172A

Plasmid

#74382

Purpose

Flag tagged human S172A MFF isoform 5 mutant

Depositor

Reuben Shaw

Article

Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

Insert

Flag-MFF (MFF Human)

Tags

Flag

Expression

Mammalian

Mutation

Ser 172 Ala (numbering based on MFF isoform 1)

Available Since

April 13, 2016

Availability

Academic Institutions and Nonprofits only
pGEM-NLS-BirA-2A-mCherry-SV40pA-FKF

Plasmid

#79889

Purpose

Donor cassette containing HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

Depositor

Tatjana Sauka-Spengler

Article

Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

Insert

HA-tagged NLS-BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene

Expression

Bacterial

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pEMS2177

Plasmid

#111892

Purpose

High copy number plasmid containing Ple344 (TUBB3 MiniPromoter) insert.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

Ple344 (TUBB3 Human)

Use

Unspecified

Promoter

Ple344 (TUBB3 MiniPromoter)

Available Since

Aug. 10, 2018

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pET41s-GST-ATF6a-AD

Plasmid

#64774

Purpose

bacterial expression of GST-tagged ATF6 (1-150)

Depositor

Joan Conaway, Ronald Conaway

Article

Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

Insert

ATF6a-AD (1-150) (ATF6 Human)

Tags

GST

Expression

Mammalian

Mutation

C-term deletion

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only
pEMS2175

Plasmid

#111890

Purpose

High copy number plasmid containing Ple342 (TUBB3 MiniPromoter) insert.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

Ple342 (TUBB3 Human)

Use

Unspecified

Promoter

Ple342 (TUBB3 MiniPromoter)

Available Since

Aug. 10, 2018

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pEMS2176

Plasmid

#111891

Purpose

High copy number plasmid containing Ple343 (TUBB3 MiniPromoter) insert.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

Ple343 (TUBB3 Human)

Use

Unspecified

Promoter

Ple343 (TUBB3 MiniPromoter)

Available Since

Aug. 10, 2018

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pGEX-GST-CHD5(Chromos)-FRAG

Plasmid

#68875

Purpose

IPTG inducible bacterial expression of CHD5 fragment

Depositor

Adrian Bracken

Article

Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

Insert

CHD5 (CHD5 Human)

Tags

GST

Expression

Bacterial

Mutation

only the two chromodomains of CHD5

Promoter

tac

Available Since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only
Cherry-SRBC

Plasmid

#69492

Purpose

Expression of fluorescently tagged SRBC in mammalian cells

Depositor

Rob Parton

Article

Gambin et al Elife. 2013 Jan 1;3:e01434. doi: 10.7554

Insert

SRBC (Cavin3 Mouse)

Tags

Cherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2016

Availability

Academic Institutions and Nonprofits only
pCDNA3 Flag MFF iso5 S155A

Plasmid

#74381

Purpose

Flag tagged human S155A MFF isoform 5 mutant

Depositor

Reuben Shaw

Article

Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

Insert

Flag-MFF (MFF Human)

Tags

Flag

Expression

Mammalian

Mutation

Ser 155 Ala (numbering based on MFF isoform 1)

Available Since

April 13, 2016

Availability

Academic Institutions and Nonprofits only
pEMS1136

Plasmid

#29056

Purpose

Expression of the eGFP reporter gene was not detected in the brain and eye.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

Ple198 (SLC6A4 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Available Since

Nov. 16, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pSBtet-6xHis-TFIIB-V5-BB

Plasmid

#242714

Purpose

Doxycycline-inducible expression of double-tagged 6xHis-TFIIB-V5 (human). Construct is resistant to Dharmacon OnTarget and Accell GTF2B siRNA pools.

Depositor

Britt Glaunsinger

Article

Gulyas et al Genes Dev. 2026 May 13. doi: 10.1101/gad

Insert

Transcription Factor IIB (GTF2B Human)

Tags

6xHis and V5

Expression

Mammalian

Mutation

Construct is resistant to Dharmacon Accell and On…

Promoter

Tight TRE promoter

Available Since

June 2, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pSBbi-GP-TFIIB(D207A)-2xStrep

Plasmid

#213920

Purpose

Sleeping beauty vector for expression of C-terminally strep-tagged TFIIB(D207A)

Depositor

Britt Glaunsinger

Article

Gulyas et al Genes Dev. 2026 May 13. doi: 10.1101/gad

Insert

TFIIB(D207A)-2xStrep (GTF2B Human)

Tags

2xStrep

Expression

Mammalian

Mutation

(1) D207A (2) Coding region changed for resistanc…

Promoter

EF-1EF1a/RPBSA

Available Since

June 2, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pSBbi-GP-TFIIB(S65A)-2xStrep

Plasmid

#213921

Purpose

Sleeping beauty vector for expression of C-terminally strep-tagged TFIIB(S65A)

Depositor

Britt Glaunsinger

Article

Gulyas et al Genes Dev. 2026 May 13. doi: 10.1101/gad

Insert

TFIIB(S65A)-2xStrep (GTF2B Human)

Tags

2xStrep

Expression

Mammalian

Mutation

(1) S65A (2) Coding region changed for resistance…

Promoter

EF-1EF1a/RPBSA

Available Since

June 2, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pSBbi-GP-TFIIB(S65E)-2xStrep

Plasmid

#213922

Purpose

Sleeping beauty vector for expression of C-terminally strep-tagged TFIIB(S65E)

Depositor

Britt Glaunsinger

Article

Gulyas et al Genes Dev. 2026 May 13. doi: 10.1101/gad

Insert

TFIIB(S65E)-2xStrep (GTF2B Human)

Tags

2xStrep

Expression

Mammalian

Mutation

(1) S65E (2) Coding region changed for resistance…

Promoter

EF-1EF1a/RPBSA

Available Since

June 2, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge