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pJ2048.2_Gs

Plasmid

#140672

Purpose

1-color stripe (“GREEN”)

Depositor

Yolanda Schaerli

Publication

Santos-Moreno et al Nat Commun. 2020 Jun 2;11(1):2746. doi:

Insert

1-color stripe (“GREEN”)

Use

Synthetic Biology

Expression

Bacterial

Available since

July 10, 2020

Availability

Academic Institutions and Nonprofits only

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pNC829

Plasmid

#20386

Depositor

Beverly Errede

Publication

Hackett et al Yeast. 2006 Apr 15. 23(5):333-49.

Insert

CFP

Expression

Yeast

Available since

March 6, 2009

Availability

Academic Institutions and Nonprofits only

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pDASH-AIK

Plasmid

#242525

Purpose

Acceptor vector I, accepting Donor I first, with attPTT-FRT

Depositor

Jose Alonso, Anna Stepanova

Publication

Zhao et al Plant Biotechnol J. 2025 Jun 10. doi: 10

Type

Empty backbone

Use

Synthetic Biology

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pPlantPOP

Plasmid

#241283

Purpose

Cloning gene libraries from oligonucleotide pools

Depositor

Sean Cutler

Publication

Dvir et al Proc Natl Acad Sci U S A. 2025 Aug 5;122

Type

Empty backbone

Use

Other

Available since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pNGFP-BC

Plasmid

#161956

Purpose

Used for rapid and efficient membrane protein precrystallization screening strategy utilizing green fluorescent protein and fluorescence-detection size-exclusion chromatography (FSEC).

Depositor

Eric Gouaux

Publication

Kawate et al Structure. 2006 Apr;14(4):673-81. doi: 1

Type

Empty backbone

Tags

GFPuv His8

Expression

Bacterial

Promoter

T7

Available since

July 15, 2025

Availability

Academic Institutions and Nonprofits only

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pCGFP-BC

Plasmid

#161959

Purpose

Used for rapid and efficient membrane protein precrystallization screening strategy utilizing green fluorescent protein and fluorescence-detection size-exclusion chromatography (FSEC).

Depositor

Eric Gouaux

Publication

Kawate et al Structure. 2006 Apr;14(4):673-81. doi: 1

Type

Empty backbone

Tags

GFPuv His8

Expression

Bacterial

Promoter

T7

Available since

July 15, 2025

Availability

Academic Institutions and Nonprofits only

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pKD016

Plasmid

#207932

Purpose

Premade plasma membrane marker, pUBQ10:PiP1;4:mTurquoise2:tHSP18.2M:FastRed, for braiding

Depositor

Alexis Maizel

Publication

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Type

Empty backbone

Expression

Plant

Available since

Nov. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pJ2042.2_invRep

Plasmid

#140688

Purpose

3-color stripe (inverted reporters)

Depositor

Yolanda Schaerli

Publication

Santos-Moreno et al Nat Commun. 2020 Jun 2;11(1):2746. doi:

Insert

3-color stripe (inverted reporters)

Use

Synthetic Biology

Expression

Bacterial

Available since

July 13, 2020

Availability

Academic Institutions and Nonprofits only

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pJ2076.2_cL

Plasmid

#140668

Purpose

cL control for toggle switch experiment

Depositor

Yolanda Schaerli

Publication

Santos-Moreno et al Nat Commun. 2020 Jun 2;11(1):2746. doi:

Insert

cL control for toggle switch experiment

Use

Synthetic Biology

Expression

Bacterial

Available since

July 10, 2020

Availability

Academic Institutions and Nonprofits only

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MTK3_002

Plasmid

#123719

Purpose

Encodes bsr (blasticidin-S deaminase) as a Type 3 part to be used in the MTK system

Depositor

Hana El-Samad

Publication

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Blasticydin Resistance

Expression

Mammalian

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pICH54011

Plasmid

#48065

Depositor

Sylvestre Marillonnet

Publication

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Available since

Dec. 3, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pICH54022

Plasmid

#48066

Depositor

Sylvestre Marillonnet

Publication

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Available since

Dec. 3, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pNC860

Plasmid

#20390

Depositor

Beverly Errede

Publication

Hackett et al Yeast. 2006 Apr 15. 23(5):333-49.

Insert

CFP

Expression

Yeast

Mutation

Linker: ek

Available since

March 6, 2009

Availability

Academic Institutions and Nonprofits only

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pDASH-DII-α1

Plasmid

#242515

Purpose

Donor II vector in DASH and alpha1 vector in GoldenBraid/DASH, with attPCC and attPTT-FRT

Depositor

Jose Alonso, Anna Stepanova

Publication

Zhao et al Plant Biotechnol J. 2025 Jun 10. doi: 10

Type

Empty backbone

Use

Synthetic Biology

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDASH-DII-ω2

Plasmid

#242518

Purpose

Donor II vector in DASH and omega2 vector in GoldenBraid/DASH, with attPCC and attPTT-FRT

Depositor

Jose Alonso, Anna Stepanova

Publication

Zhao et al Plant Biotechnol J. 2025 Jun 10. doi: 10

Type

Empty backbone

Use

Synthetic Biology

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR_TiTRE

Plasmid

#172128

Purpose

Entry vector for tightly controlled gene expression by a tetracycline-responsive promoter

Depositor

Kenji Kontani

Publication

Araki et al J Biol Chem. 2021 Nov 18:101428. doi: 10

Type

Empty backbone

Use

Gateway Cloning

Expression

Mammalian

Promoter

tight TRE

Available since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pAIP4

Plasmid

#233686

Purpose

Cloning vector with an N-terminal fusion of 10x His-tagged SUMO-1, followed by a Golden Gate assembly site for seamless insertion.

Depositor

Christopher Bahl

Publication

Cárcamo Noriega et al Protein Sci. 2026 Sep;35(9):e70746. doi:

Insert

Smt3 (SMT3 )

Tags

HisTag

Promoter

T7

Available since

April 7, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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proinsulin-EYFP

Plasmid

#218952

Purpose

Expresses proinsulin fused to EYFP in bacteria

Depositor

Benjamin Glick

Insert

proinsulin-EYFP

Tags

proinsulin

Expression

Bacterial

Promoter

T5

Available since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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AA011

Plasmid

#215973

Purpose

Fragmid fragment: (N' terminus) for C>T base editing

Depositor

John Doench

Publication

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

Start; BPNLS_v1.1; rAPOBEC1_v1.1; Linker_v1.1

Use

Fragment

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA013

Plasmid

#215975

Purpose

Fragmid fragment: (N' terminus) for A>G base editing

Depositor

John Doench

Publication

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

Start; BPNLS_v1.1; ABE8e_v1.1; Linker_v1.1

Use

Fragment

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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