We narrowed to 14,439 results for: NTS

1. pB[vasa-Cas9 PUb-ZsGreen]

   Plasmid

   #169012

   Purpose

   For germline transformation. Expresses Cas9 in germ cells and ZsGreen marker in all cells.

   Depositor

   Max Scott

   Article

   Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

   Insert

   Cas9
   

   Use

   Piggybac

   Expression

   Insect

   Promoter

   Drosophila melanogaster vasa

   Available Since

   April 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. CABE-T1.2

   Plasmid

   #193258

   Purpose

   Expression of CABE-T1.2 base editor in mammalian cells, CMV promoter

   Depositor

   Nicole Gaudelli

   Article

   Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

   Insert

   CABE-T1.2
   

   Expression

   Mammalian

   Mutation

   D10A (Cas9), TadA mutations within

   Promoter

   CMV

   Available Since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOPS0381

   Plasmid

   #133231

   Purpose

   Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.

   Depositor

   Philip Poole

   Article

   Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117

   Insert

   constructed with constitutive promoter PsNifH (pOGG043), sfGFP (pOGG037) and T-pharma (pOGG003)
   

   Expression

   Bacterial

   Mutation

   Domesticated for Golden Gate cloning

   Available Since

   April 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. HypaCas9

   Plasmid

   #138557

   Purpose

   Expresses human codon-optimized hyper-accurate SpCas9 and blasticidin resistance: EFS promoter-HypaCas9-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

   Insert

   HypaCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   N692A, M694A, Q695A, H698A

   Promoter

   EFS

   Available Since

   June 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNA5/FRT/TO_CEP41_HaloTag9_T2A_EGFP

   Plasmid

   #169328

   Purpose

   Expression of HaloTag9 located at the microtubules of mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   CEP41-HaloTag9 (CEP41 Human)
   

   Tags

   HaloTag9

   Expression

   Mammalian

   Mutation

   HaloTag9 = HaloTag7-Q165H-P174R

   Available Since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. KHBD00188

   Plasmid

   #34340

   Depositor

   Bart Deplancke

   Article

   Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

   Insert

   CG1034 (bcd Fly)
   

   Use

   Gateway donor vector

   Available Since

   Jan. 23, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR221 NLRP1

   Plasmid

   #166823

   Purpose

   Gateway pDONR vector containing NLRP1 (no stop codon)

   Depositor

   Daniel Bachovchin

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Gateway shuttling vector

   Available Since

   March 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNA5/FRT/TO_TOMM20_HaloTag9

   Plasmid

   #169335

   Purpose

   Expression of HaloTag9 at the mitochondrial surface of mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   TOMM20-HaloTag9 (TOMM20 Human)
   

   Tags

   HaloTag9

   Expression

   Mammalian

   Mutation

   HaloTag9 = HaloTag7-Q165H-P174R

   Available Since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-GFPLaminA-L530P

   Plasmid

   #32710

   Depositor

   Wendy Bickmore

   Article

   Gilchrist et al BMC Cell Biol. 2004 Dec 13;5(1):46.

   Insert

   Lamin A (LMNA Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   L530P

   Promoter

   CMV

   Available Since

   Jan. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pCMV-BE4max-NRCH

    Plasmid

    #136920

    Purpose

    Expresses BE4max-NRCH in mammalian cells

    Depositor

    David Liu

    Article

    Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

    Insert

    BE4max-NRCH
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    see manuscript

    Promoter

    CMV

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-BE4max-NRRH

    Plasmid

    #136918

    Purpose

    Expresses BE4max-NRRH in mammalian cells

    Depositor

    David Liu

    Article

    Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

    Insert

    BE4max-NRRH
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    see manuscript

    Promoter

    CMV

    Available Since

    April 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-BE4max-NRTH

    Plasmid

    #136919

    Purpose

    Expresses BE4max-NRTH in mammalian cells

    Depositor

    David Liu

    Article

    Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

    Insert

    BE4max-NRTH
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    see manuscript

    Promoter

    CMV

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV-U6-gRNA-UbC-eGFP-P2A-Bsr

    Plasmid

    #83925

    Purpose

    Lentiviral SpCas9-gRNA expression vector with eGFP-P2A-BlastR

    Depositor

    Charles Gersbach

    Article

    Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

    Inserts

    eGFP
    Bsr
    

    Use

    CRISPR and Lentiviral

    Tags

    P2A

    Promoter

    hUbC

    Available Since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOPS0382

    Plasmid

    #133232

    Purpose

    Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.

    Depositor

    Philip Poole

    Article

    Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117

    Insert

    constructed with constitutive promoter PsNifH (pOGG043), mCherry (EC15071) and T-pharma (pOGG003)
    

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Available Since

    April 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. ZIKV_Sensor_27B_LacZ

    Plasmid

    #75006

    Purpose

    Toehold switch sensor to detect Zika Virus

    Depositor

    James Collins, Alexander Green

    Article

    Pardee et al Cell. 2016 May 6. pii: S0092-8674(16)305

    Insert

    ZIKV_sensor_27B_LacZ
    

    Tags

    LacZ

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. p3015b

    Plasmid

    #209317

    Purpose

    Expresses a sgRNA template with a protospacer cloning site flanked by BsaI restriction sites for easy insertion of a targeting cassette.

    Depositor

    Thomas Hooven

    Article

    Gopalakrishna et al Commun Biol. 2023 Jun 9;6(1):620. doi: 1

    Insert

    Group B Streptococcus-compatible single guide RNA
    

    Expression

    Bacterial

    Promoter

    Xyl/tet promoter that is constitutively active

    Available Since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. HsGRK6-mVenus

    Plasmid

    #137780

    Purpose

    Visualization of GRK6

    Depositor

    Dorus Gadella

    Article

    Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

    Insert

    Human GRK6 (GRK6 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pENTR-JNKKTRmRuby2

    Plasmid

    #59148

    Purpose

    ORF encoding JNK KTR mRuby2 flanked by Gateway sequences

    Depositor

    Markus Covert

    Article

    Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

    Insert

    JNK Kinase Translocation Reporter (MAPK8 Mouse, Human)
    

    Tags

    mRuby2

    Available Since

    Sept. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pQE-BR1

    Plasmid

    #160333

    Purpose

    This plasmid encodes an engineered oxygen-independent flavin binding fluorescent protein with N-terminal 6xHis tag. Its quantum yield is improved compared to wild type.

    Depositor

    Sang Taek Jung

    Article

    Ko et al J Agric Food Chem. 2019 Oct 30;67(43):12

    Insert

    BR1
    

    Tags

    6xHis tag

    Expression

    Bacterial

    Promoter

    T5

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. CABE-T3.1

    Plasmid

    #193267

    Purpose

    Expression of CABE-T3.1 base editor in mammalian cells, CMV promoter

    Depositor

    Nicole Gaudelli

    Article

    Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

    Insert

    CABE-T3.1
    

    Expression

    Mammalian

    Mutation

    D10A (Cas9), TadA mutations within

    Promoter

    CMV

    Available Since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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