We narrowed to 12,721 results for: CAR

1. pUPD2 AtU6-26:AsCas12a_DR (GB1442)

   Plasmid

   #160574

   Purpose

   AsCas12a direct repeat sequence fused to the A. thaliana U6-26 promoter used for the assembly of gRNA expression cassettes.

   Depositor

   Diego Orzaez

   Article

   Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

   Insert

   AtU6-26:AsCas12a_DR
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUPD2 LbCas12a (GB1439)

   Plasmid

   #160573

   Purpose

   Human codon optimized coding sequence of Cas12a from Lachnospiraceae CRISPR system (LbCas12a).

   Depositor

   Diego Orzaez

   Article

   Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

   Insert

   LbCas12a
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUPD2 AsCas12a (GB1438)

   Plasmid

   #160572

   Purpose

   Human codon optimized coding sequence of Cas12a from Acidominococcus sp (AsCas12a).

   Depositor

   Diego Orzaez

   Article

   Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

   Insert

   AsCas12a
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-EGxxFP-PIGA

   Plasmid

   #153958

   Purpose

   Used for EGxxFP assay with PIGA intron 5 and exon 6 sequences

   Depositor

   Hiroyuki Konishi

   Article

   Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

   Insert

   PIGA (a 401-bp fragment from intron 5 and exon 6)
   

   Use

   A plasmid specific for egxxfp assay

   Tags

   N/A

   Expression

   Mammalian

   Mutation

   No mutation

   Promoter

   CAG promoter

   Available since

   Dec. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-EGxxFP-CD55

   Plasmid

   #153959

   Purpose

   Used for EGxxFP assay with CD55 intron 3, exon 4, and intron 4 sequences

   Depositor

   Hiroyuki Konishi

   Article

   Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

   Insert

   CD55 (a 1,016-bp fragment from intron 3, exon 4, and intron 4)
   

   Use

   A plasmid specific for egxxfp assay

   Tags

   N/A

   Expression

   Mammalian

   Mutation

   No mutation

   Promoter

   CAG promoter

   Available since

   Dec. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLKO-Cre sgUroD v4

   Plasmid

   #158036

   Purpose

   lenti-viral construct with Cre recombinase and U6 driven sgRNA against mouse UroD

   Depositor

   Daniel Schramek

   Article

   Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

   Insert

   UroD sgRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Oct. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pLKO-Cre sgItgb5 v3

   Plasmid

   #158038

   Purpose

   lenti-viral construct with Cre recombinase and U6 driven sgRNA against mouse Itgb5

   Depositor

   Daniel Schramek

   Article

   Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

   Insert

   Itgb5 sgRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 30, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pLKO-Cre sgRipk4 v3

   Plasmid

   #158035

   Purpose

   lenti-viral construct with Cre recombinase and U6 driven sgRNA against mouse Ripk4

   Depositor

   Daniel Schramek

   Article

   Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

   Insert

   Ripk4 sgRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. eNS2B47NS3 S135A

   Plasmid

   #155318

   Purpose

   DENV4 47 peptides from NS2B cofactor linked to NS3 full length by VKTQR linker S135A mutant

   Depositor

   Dahai Luo

   Article

   Phoo et al Antiviral Res. 2020 Oct;182:104900. doi:

   Insert

   DENV4 NS3 full length protein linked with 47 peptides from NS2B cofactor
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   S135A

   Promoter

   M13

   Available since

   Sept. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCS2 Halo-Tev- HSPB7(zebrafish) C49S

    Plasmid

    #108914

    Purpose

    Express C49S mutant of Zebrafish Halo-Tev-HSPB7 in mammalian cells

    Depositor

    Yimon Aye

    Article

    Surya et al ACS Chem Biol. 2018 Feb 8. doi: 10.1021/

    Insert

    Halo-TEV-HSPB7
    

    Use

    Tags

    Halo-TEV

    Expression

    Mammalian

    Mutation

    Cysteine 49 to Serine

    Promoter

    SP6

    Available since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. Lenti-XYLT2-sgRNA

    Plasmid

    #154862

    Purpose

    Lentiviral expression of Cas9 and sgRNA targeting XYLT2

    Depositor

    Yihong Ye

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

    Insert

    XYLT2 sgRNA (XYLT2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPN455

    Plasmid

    #137872

    Purpose

    Expression of gRNA targeting POGZ for CRISPRa; U6 promoter

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

    Insert

    POGZ (POGZ Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPN447

    Plasmid

    #137871

    Purpose

    Expression of gRNA targeting POGZ for CRISPRi; U6 promoter

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

    Insert

    POGZ (POGZ Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA5-FRT-TO-EGFP-RIP2_3K

    Plasmid

    #131205

    Purpose

    mammalian expression, tet R

    Depositor

    Thomas Kufer

    Article

    Ellwanger et al Life Sci Alliance. 2019 Jul 26;2(4). pii

    Insert

    RIPK2 K209/410/538R (RIPK2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    K209/410/538R

    Promoter

    pCMV

    Available since

    Sept. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSR40.16

    Plasmid

    #125083

    Purpose

    Expression of ompR(1-140aa)-ccaR(130-234aa) chimera under PLtetO-1, output Promoter PcpcG2-172

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

    Inserts

    ompR(REC)-ccaR(DBD)140
    sfgfp
    tetR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKD263

    Plasmid

    #125094

    Purpose

    Expression of torR(1-134aa)-ccaR(127-234aa), ompR137 chimera under PLtetO-1, output Promoter PcpcG2-172

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

    Inserts

    torR(REC)-ccaR(DBD)137
    sfgfp
    tetR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSR40.25

    Plasmid

    #125085

    Purpose

    Expression of ompR(1-110aa)-ccaR(107-234aa) chimera under PLtetO-1, output Promoter PcpcG2-172

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

    Inserts

    ompR(REC)-ccaR(DBD)110
    sfgfp
    tetR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTBL436 HEK293site3EQR sgRNA

    Plasmid

    #126445

    Purpose

    To cut the human HEK293 site3 locus for integration.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Insert

    spacer against human HEK293 site3 with NGAG PAM
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6

    Available since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSR40.28

    Plasmid

    #125087

    Purpose

    Expression of ompR(1-138aa)-ccaR(128-234aa) chimera under PLtetO-1, output Promoter PcpcG2-172

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

    Inserts

    ompR(REC)-ccaR(DBD)138
    sfgfp
    tetR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSR40.26

    Plasmid

    #125086

    Purpose

    Expression of ompR(1-113aa)-ccaR(110-234aa) chimera under PLtetO-1, output Promoter PcpcG2-172

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

    Inserts

    OmpR(REC)-ccaR(DBD)113
    sfgfp
    tetR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge