We narrowed to 18,937 results for: Tec

1. pcDNA3-FKBP-C-mSOG-J-alpha-IRES-mCherry

   Plasmid

   #136603

   Purpose

   Encodes FKBP tagged with the C-terminal fragment of split-miniSOG (mSOG-Ja95-140).

   Depositor

   John Ngo

   Article

   Boassa et al Cell Chem Biol. 2019 Oct 17;26(10):1407-

   Insert

   FKBP-C-mSOG-J-alpha
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUSE-RapR-Src(KD)-myc

   Plasmid

   #25934

   Depositor

   Klaus Hahn

   Article

   Karginov et al Nat Biotechnol. 2010 Jul;28(7):743-7. do

   Insert

   RapR-Src (Fkbp1a Mouse)
   

   Expression

   Mammalian

   Mutation

   This pUSE-RapR-Src(KD)-myc plasmid contains the k…

   Available Since

   Oct. 26, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-ABE-NT-sgRNA

   Plasmid

   #112734

   Purpose

   AAV inverted terminal repeat based vector plasmid encoding E. coli TadA, the N-terminal half of nCas9 and sgRNA

   Depositor

   Jin-Soo Kim

   Article

   Ryu et al Nat Biotechnol. 2018 Jul;36(6):536-539.

   Insert

   ABE-NT-sgRNA
   

   Use

   AAV

   Available Since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-eGFP-dSaCas9-blast

   Plasmid

   #191653

   Purpose

   Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9), eGFP, and the blasticidin resistance gene.

   Depositor

   Moshe Szyf

   Article

   Sapozhnikov et al Sci Rep. 2023 Feb 9;13(1):2280. doi: 10.

   Insert

   eGFP-T2A-dSaCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   3XFLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCfB3052(gRNA X-4, XI-3, XII-5)

   Plasmid

   #73294

   Purpose

   EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at sites X-4, XI-3, and XII-5

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Insert

   guiding RNA
   

   Use

   CRISPR; Grna

   Expression

   Yeast

   Available Since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGS1021

   Plasmid

   #59575

   Purpose

   Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal Twin-Strep tag and a TRP1 selection marker

   Depositor

   Monika Golas, Bjoern Sander

   Article

   Rai et al Mol Biotechnol. 2014 Jun 27.

   Insert

   Twin-Strep tag optimized for PCR amplification followed by TRP1 selection marker
   

   Use

   E. coli cloning vector

   Tags

   Twin-Strep tag

   Promoter

   None

   Available Since

   Aug. 28, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLCHKO

   Plasmid

   #155048

   Purpose

   Lentiviral backbone for cloning and expressing U6 driven hybrid guide (hg)RNAs with BfuAI cloning sites and puromycin selection.

   Depositor

   Benjamin Blencowe, Jason Moffat

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. Bact2-APEX2-GBP in pDEST-Tol2-pA2-acrys-cherry

   Plasmid

   #67668

   Purpose

   Zebrafish expression vector for ubiquitous expression of APEX2-GBP

   Depositor

   Rob Parton

   Article

   Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

   Insert

   APEX2-GBP
   

   Use

   Zebrafish expression with tol2

   Available Since

   Dec. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-hA3F-BE3

   Plasmid

   #113414

   Purpose

   Expresses hA3F-BE3 in mammalian cells

   Depositor

   Jia Chen

   Article

   Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

   Insert

   hA3F-BE3 (APOBEC3F S. pyogenes and Bacteriophage PBS2, Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRSF-G1(6CNW)RS

    Plasmid

    #222873

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of 6-Cyano-L-Tryptophan (6-CN-Trp) into proteins in E. coli in response to the amber (TAG) codon

    Depositor

    Thomas Huber

    Article

    Qianzhu et al Angew Chem Int Ed Engl. 2024 Dec 4:e2024

    Insert

    tRNA synthetase
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRB117.2

    Plasmid

    #181949

    Purpose

    aTc-inducible expression of NarL with N-terminal mNeonGreen fusion. Also contains mCherry under NarL-controlled promoter PdcuS

    Depositor

    Jeffrey Tabor

    Article

    Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11

    Insert

    mNG-NarL; mCherry; tetR (narL, AX04_RS23485 Synthetic, narL - E. coli)
    

    Tags

    mNeonGreen

    Expression

    Bacterial

    Mutation

    See Depositor Comments Below

    Promoter

    mNG-NarL-PLtetO-1; mCherry-PdcuS; tetR-J23106

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLEXm-GST-ATG14

    Plasmid

    #99329

    Purpose

    Component for mammalian expression of recombinant PI3KC3-C1 complex

    Depositor

    James Hurley

    Article

    Baskaran et al Elife. 2014 Dec 9;3. doi: 10.7554/eLife.

    Insert

    ATG14 (ATG14 Human)
    

    Tags

    GST-TEV tag

    Expression

    Mammalian

    Mutation

    Codon optimized

    Promoter

    CMV

    Available Since

    Aug. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. AA025

    Plasmid

    #216005

    Purpose

    Fragmid fragment: (Cas protein) NG PAM

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    Cas9-NG_v1.1 [Sp]
    

    Use

    CRISPR; Fragment

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pShCAST(Cas12k-TniQ-TniQ)-sgRNA_entry (CJT12)

    Plasmid

    #181788

    Purpose

    Expresses 3-component ShCAST containing a Cas12k-TniQ-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    ShTnsB, ShTnsC, ShCas12k-ShTniQ-ShTniQ
    

    Expression

    Bacterial

    Mutation

    nAniI = K227M, F80K, L232K

    Promoter

    Lac and J23119

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. SaCas9 TadCBEa

    Plasmid

    #193844

    Purpose

    Express TadCBEa (with SaCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    SaCas9 TadCBEa
    

    Expression

    Mammalian

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. AA299

    Plasmid

    #216029

    Purpose

    Fragmid fragment: (Cas protein) NNGG PAM

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    Cas9_v1.1 [Slug]
    

    Use

    CRISPR; Fragment

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. AA296

    Plasmid

    #216028

    Purpose

    Fragmid fragment: (Cas protein) NNNNCC PAM

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    Nme2Cas9_v1.1 [Nme2]
    

    Use

    CRISPR; Fragment

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. AA180

    Plasmid

    #216023

    Purpose

    Fragmid fragment: (Cas protein) NGRR(T) PAM

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    Cas9_v1.1 [Sa]
    

    Use

    CRISPR; Fragment

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. AA301

    Plasmid

    #216030

    Purpose

    Fragmid fragment: (Cas protein) NNGG PAM

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    Cas9_v1.1 [Sauri]
    

    Use

    CRISPR; Fragment

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. AA027

    Plasmid

    #216007

    Purpose

    Fragmid fragment: (Cas protein) nearly PAM-less

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    Cas9-SpRY_v1.1 [Sp]
    

    Use

    CRISPR; Fragment

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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