We narrowed to 7,286 results for: 11

1. pAAV-hSyn-GFP-IRES-hSNCA (A11P/V70P)

   Plasmid

   #185717

   Purpose

   AAV expression of GFP and human α-Synuclein with A11P and V70P mutations from hSyn promoter

   Depositor

   Jun Ding

   Article

   Albarran et al Nat Neurosci. 2023 Jun;26(6):997-1007. d

   Insert

   synuclein, alpha (SNCA Human)
   

   Use

   AAV

   Mutation

   Changed Ala 11 to Pro, Val 70 to Pro

   Promoter

   hSyn

   Available Since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. His-MBP-CHMP1B, S2C

   Plasmid

   #184803

   Purpose

   Bacterial expression of CHMP1B (S2C mutation)

   Depositor

   James Hurley

   Article

   Cada et al Proc Natl Acad Sci U S A. 2022 Jul 19;11

   Insert

   CHMP1B (CHMP1B Human)
   

   Tags

   His6-MBP

   Expression

   Bacterial

   Mutation

   S2C

   Promoter

   T7

   Available Since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX-2T-CBP(1603-2678)

   Plasmid

   #183774

   Purpose

   Express GST-CBP(1603-2678) in E. coli. Purified GST-CBP(1603-2678) was used for in vitro acetylation assays.

   Depositor

   Peter Harte

   Article

   Tie et al Mol Cell Biol. 2012 Jun;32(12):2323-34.

   Insert

   nej (nej Fly)
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSUMO GLTP

   Plasmid

   #170732

   Purpose

   The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

   Depositor

   Rhoderick Brown

   Article

   Ochoa-Lizarralde et al J Biol Chem. 2018 Oct 26;293(43):16709-1

   Insert

   GLTP (GLTP Human)
   

   Tags

   SUMO

   Expression

   Bacterial

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. sgRNA targeting human SLC12A5 gene stop codon

   Plasmid

   #158577

   Purpose

   For generate double-strand DNA break near the stop codon of the human SLC12A5 gene that encodes neuronal chloride transporter KCC2 protein.

   Depositor

   Rudolf Jaenisch

   Article

   Tang et al Sci Transl Med. 2019 Jul 31;11(503). pii

   Insert

   sgRNA targeting human SLC12A5 gene stop codon
   

   Use

   CRISPR

   Tags

   Cas9 and GFP

   Expression

   Mammalian

   Available Since

   Sept. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. Cry2 mcherry Gamma 9 in pcDNA3.1

   Plasmid

   #64208

   Purpose

   mCherry fused between Cry2 and gamma 9 to study cell migration

   Depositor

   Narasimhan Gautam

   Article

   O'Neill et al Mol Biol Cell. 2014 Aug 1;25(15):2305-14

   Inserts

   Cry2 (CRY2 Mustard Weed)
   gamma cone (GNGT2 Human)
   

   Tags

   mcherry and n/a

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 2, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA3.1-nAChr-Alpha6-CFP

   Plasmid

   #50482

   Purpose

   this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF

   Depositor

   Henry Lester

   Article

   Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

   Insert

   nAChr-alpha6 (Chrna6 Mouse)
   

   Tags

   CFP fusion in M3-M4 loop (after residue A405)

   Expression

   Mammalian

   Mutation

   Gly-Ala-Gly flexible linker flanking the CFP open…

   Promoter

   CMV

   Available Since

   Jan. 13, 2014

   Availability

   Academic Institutions and Nonprofits only

8. FUW-TetON-Sox10

   Plasmid

   #115242

   Purpose

   Lentiviral plasmid for tet-inducible expression of Sox10 (generated from plasmid #20321)

   Depositor

   Catherine Verfaillie

   Article

   Garcia-Leon et al Stem Cell Reports. 2018 Feb 13;10(2):655

   Insert

   SOX10 (SOX10 Human)
   

   Use

   Lentiviral

   Available Since

   Nov. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. W118-1_hZBTB7B

   Plasmid

   #180380

   Purpose

   lentiviral transduction of human ZBTB7B gene

   Depositor

   Huda Zoghbi

   Article

   Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

   Insert

   ZBTB7B (ZBTB7B Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. lentiCRISPR v2-sgSLC7A11/xCT-1

    Plasmid

    #161818

    Purpose

    knock out SLC7A11/xCT in mammalian cells

    Depositor

    Boyi Gan

    Article

    Liu et al Nat Cell Biol. 2020 Apr;22(4):476-486. d

    Insert

    SLC7A11 solute carrier family 7 member 11 xCT (SLC7A11 Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    Dec. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. EGFP-HsBIN1_tv8

    Plasmid

    #176012

    Purpose

    Mammalian expression of Human EGFP-Bin1 (transcriptional variant 8, muscle specific). Parton lab clone HBH

    Depositor

    Rob Parton

    Article

    Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

    Insert

    Bin1
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Nov. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pESF140_HisSpy_DDB1∆B

    Plasmid

    #124213

    Purpose

    Insect cell expression vector for His6, Spy tagged hsDDB1∆B

    Depositor

    Eric Fischer

    Article

    Nowak et al Nat Chem Biol. 2018 Jul;14(7):706-714. d

    Insert

    DNA damage-binding protein 1 (DDB1 Human)
    

    Tags

    His6-Spy

    Expression

    Insect

    Mutation

    internal deletion of BPB domain to aid crystalliz…

    Available Since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. lentiCRISPR v2-sgSLC7A11/xCT-2

    Plasmid

    #161819

    Purpose

    knock out SLC7A11/xCT in mammalian cells

    Depositor

    Boyi Gan

    Article

    Liu et al Nat Cell Biol. 2020 Apr;22(4):476-486. d

    Insert

    solute carrier family 7 member 11 (SLC7A11 Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    Dec. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSAM2_mCherry_Gata6

    Plasmid

    #72694

    Purpose

    Doxycyline regulated expression of Gata6 in mammalian cells

    Depositor

    Timothy Kamp

    Article

    Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

    Insert

    Gata 6 (Gata6 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV-Doxycycline

    Available Since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTRE-BI-hGRAD

    Plasmid

    #207837

    Purpose

    Inducible hGRAD Plasmids for rapid depletion of GFP-tagged proteins in mammalian cells

    Depositor

    Michaela Mueller-McNicoll

    Article

    Arnold et al J Cell Biol. 2024 Feb 5;223(2):e20230403

    Inserts

    mCherry
    hFBXW11 (FBXW11 Human)
    VHH-GFP4
    

    Expression

    Mammalian

    Mutation

    215 amino acids of the full length protein

    Promoter

    TRE3G BI promoter

    Available Since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pME-GcAMP5

    Plasmid

    #126569

    Purpose

    Multisite gateway middle entry clone containing zebrafish calcium reporter GcAMP5. Parton lab clone ANR.

    Depositor

    Rob Parton

    Article

    Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

    Insert

    GcAMP5
    

    Use

    Gateway entry clone

    Available Since

    Nov. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSAM2_mCherry_Gata4

    Plasmid

    #72690

    Purpose

    Doxycyline regulated expression of Gata4 in mammalian cells

    Depositor

    Timothy Kamp

    Article

    Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

    Insert

    Gata 4 (Gata4 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV-Doxycycline

    Available Since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. actc1b-GcAMP5

    Plasmid

    #126563

    Purpose

    Muscle specific expression of the calcium reporter GcAMP5 in zebrafish. Parton lab clone APT

    Depositor

    Rob Parton

    Article

    Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

    Insert

    GcAMP5
    

    Available Since

    Nov. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONR221-SLC39A11_STOP

    Plasmid

    #161307

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC39A11 (SLC39A11 Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 15, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. H3S10ph biosensor

    Plasmid

    #120809

    Purpose

    FRET biosensor. To monitor histone H3 Serine 10 phosphorylation in mammalian cells by FRET

    Depositor

    Yingxiao Wang

    Article

    Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

    Insert

    Mouse histone H3-CFP-FHA2-histone H3 peptide (1-14aa)-YFP
    

    Expression

    Mammalian

    Mutation

    Threonine 3, Threonine 6, and Threonine 11 are mu…

    Promoter

    CMV

    Available Since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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