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pBOB-C-Flag-GK5 (1-287)

Plasmid

#134262

Purpose

Lentivector encoding Flag-tagged GK5 truncation (amino acids 1-287)

Depositor

Bruce Beutler

Publication

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

mouse Gk5 amino acids 1-287

Promoter

CMV

Available since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pBOB-C-Flag-GK5_v2

Plasmid

#134260

Purpose

Lentivector encoding Flag-tagged GK5 (isoform 2)

Depositor

Bruce Beutler

Publication

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

mouse Gk5 isoform 2; 534 amino acids

Promoter

CMV

Available since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pBOB-C-Flag-GK5_v1

Plasmid

#134257

Purpose

Lentivector encoding Flag-tagged GK5 (isoform 1)

Depositor

Bruce Beutler

Publication

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

mouse Gk5 isoform 1; 516 amino acids

Promoter

CMV

Available since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pBOB-C-Flag-GK5 (287-396)

Plasmid

#134265

Purpose

Lentivector encoding Flag-tagged GK5 truncation (amino acids 287-396)

Depositor

Bruce Beutler

Publication

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

mouse Gk5 amino acids 287-396

Promoter

CMV

Available since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

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pFl Strep-sumo-TEV-hAgo2

Plasmid

#136235

Purpose

Expresses human Argonaute-2 in insect cells

Depositor

Leemor Joshua-Tor

Publication

Elkayam et al Cell. 2012 Jul 6;150(1):100-10. doi: 10.

Insert

human Argonaute-2 (AGO2 Human)

Tags

Strep Sumo

Expression

Insect

Promoter

polyhedrin

Available since

May 14, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-eGFP-MinD

Plasmid

#133622

Purpose

Expression vector for the Escherichia coli MinD with an N-terminal fusion of a His-tag for purification and an EGFP for visualization.

Depositor

Petra Schwille

Publication

Zieske et al FEBS Lett. 2014 Aug 1;588(15):2545-9. do

Insert

EGFP-MinD (minD E. coli)

Tags

His

Expression

Bacterial

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pCW-IRF4-Puro

Plasmid

#123323

Purpose

Lentiviral vector for expression of IRF4 under a Doxycycline inducible promoter

Depositor

Eva Gottwein

Publication

Patil et al Blood. 2018 Aug 9;132(6):577-586. doi: 1

Insert

IRF4 (IRF4 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

several silent mutations to disrupt sgRNA targeti…

Promoter

Doxycycline inducible minimal CMV

Available since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pR I-Ab beta Derp1-117

Plasmid

#113639

Purpose

Insect cell expression vector for I-Ab beta chain fused to Der p 1 117-127 antigenic peptide

Depositor

James Moon

Publication

Moon et al Methods Mol Biol. 2018;1799:165-181. doi

Insert

I-Ab beta Derp1-117 (H2-Ab1 Mouse)

Tags

6x His tag, fused to Der p 1 (117-127) peptide vi…

Expression

Insect

Mutation

transmembrane and cytoplasmic domains truncated

Promoter

Metallothionein

Available since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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fPE7max

Plasmid

#260756

Purpose

For fPE7max expression

Depositor

Xue Gao

Publication

Sun et al Nat Biotechnol. 2026 Jun 30. doi: 10.103

Insert

fLa

Expression

Fungal

Available since

Sept. 11, 2026

Availability

Academic Institutions and Nonprofits only

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pLG-mLRRC45 (209 a.a.)

Plasmid

#240068

Purpose

ITT reporter plasmid for cold stress

Depositor

Hodaka Fujii

Publication

Maruyama et al Biol Methods Protoc. 2025 Jun 14;10(1):b

Insert

LRRC45 C-terminal 209 a.a. (Lrrc45 Mouse)

Use

Retroviral

Tags

LexA DNA-binding (DB) domain + Gal4 transactivati…

Expression

Mammalian

Promoter

LTR

Available since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pLG-mCCDC91 (198 a.a.)

Plasmid

#240069

Purpose

ITT reporter plasmid for cold stress

Depositor

Hodaka Fujii

Publication

Maruyama et al Biol Methods Protoc. 2025 Jun 14;10(1):b

Insert

CCDC91 C-terminal 198 a.a. (Ccdc91 Mouse)

Use

Retroviral

Tags

LexA DNA-binding (DB) domain + Gal4 transactivati…

Expression

Mammalian

Promoter

LTR

Available since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pFSW-BsmBI-shRNA-hSyn-GFP

Plasmid

#220436

Purpose

Lentiviral shRNA cloning backbone with GFP

Depositor

Paul Blainey

Publication

Le et al Cell Rep. 2025 Jun 24;44(7):115889. doi:

Type

Empty backbone

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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KNtp-SUR1 core fusion

Plasmid

#216212

Purpose

for expression of KNtp-SUR1 fusion protein in BacMam system

Depositor

Lei Chen

Publication

Wang et al Front Pharmacol. 2022 Jun 30;13:929684.

Insert

SUR1 core

Tags

GFP-MBP

Expression

Mammalian

Mutation

deletion of TMD0

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pJ2039_N2only

Plasmid

#140682

Purpose

Control (“C”) lacking sg-3

Depositor

Yolanda Schaerli

Publication

Santos-Moreno et al Nat Commun. 2020 Jun 2;11(1):2746. doi:

Insert

Control (“C”) lacking sg-3

Use

Synthetic Biology

Expression

Bacterial

Available since

July 13, 2020

Availability

Academic Institutions and Nonprofits only

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pJ2040_N2only

Plasmid

#140683

Purpose

Control (“C”) lacking sg-4

Depositor

Yolanda Schaerli

Publication

Santos-Moreno et al Nat Commun. 2020 Jun 2;11(1):2746. doi:

Insert

Control (“C”) lacking sg-4

Use

Synthetic Biology

Expression

Bacterial

Available since

July 13, 2020

Availability

Academic Institutions and Nonprofits only

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pBSWt1-Dest

Plasmid

#59764

Purpose

PCR template for amplifying the attL1-em7-kanr-attL2-pA cassette

Depositor

Peter Koopman

Publication

Zhao et al Dev Dyn. 2014 Jun 12. doi: 10.1002/dvdy.

Insert

attL1-em7-kanR-attL2-pA

Available since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pTmaM4

Plasmid

#67714

Purpose

Expression vector hexahistidine tagged variant of the Thermotoga maritima M-domain protein

Depositor

Robert Batey, Jeffrey Kieft

Publication

Kieft et al RNA. 2004 Jun;10(6):988-95.

Insert

Thermotoga marítima Ffh-M domain

Tags

hexahistidine tag

Expression

Bacterial

Promoter

T7 RNA polymerase

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pGST2-yVps23-UEV_C133A

Plasmid

#45924

Depositor

James Hurley

Publication

Ren et al EMBO J. 2011 Jun 1;30(11):2130-9. doi: 1

Insert

yeast Vps23-UEV(1-160) (STP22 Budding Yeast)

Tags

GST

Expression

Bacterial

Mutation

C133A

Available since

July 29, 2013

Availability

Academic Institutions and Nonprofits only
pJT104

Plasmid

#25088

Depositor

Christopher Voigt

Publication

Tabor et al Cell. 2009 Jun 26. 137(7):1272-81.

Insert

Edge detector delta luxI

Use

Synthetic Biology

Expression

Bacterial

Available since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only

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XP31 Vox

Plasmid

#16972

Depositor

Randall Moon

Publication

Schmidt et al Development. 1996 Jun;122(6):1711-21. do

Insert

Vox (ventx2.1.L Frog)

Expression

Bacterial

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only