Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 7,762 results for: loxP

Showing: 7141 - 7160 of 7762 results

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pBiT3.1-N_LRRK2:2142-2527

Plasmid

#211112

Purpose

Mammalian expression of human LRRK2 fragment R2142 to E2527. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

LRRK2:R2142-E2527 (LRRK2 Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

M2397T natural variant (VAR_024965)

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_BTRC:1-605

Plasmid

#211101

Purpose

Mammalian expression of full-length human BTRC. a N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

BTRC:M1-R605 (BTRC Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_DDB1:1-1140

Plasmid

#211147

Purpose

Mammalian expression of full-length human DDB1. loop deletion amino acids I396-D689. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

DDB1:M1-H1140 (DDB1 Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

loop deletion I396-D689

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_EIF3B:186-685

Plasmid

#211205

Purpose

Mammalian expression of human EIF3B fragment V186 to T685. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

EIF3B:V186-T685 (EIF3B Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_EIF3I:1-325

Plasmid

#211206

Purpose

Mammalian expression of full-length human EIF3I. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

EIF3I:M1-A325 (EIF3I Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_DCAF12:1-453

Plasmid

#211198

Purpose

Mammalian expression of full-length human DCAF12 N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

DCAF12:M1-S453 (DCAF12 Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

R131Q natural variant (VAR_035322)

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_DNAI1:1-699

Plasmid

#211201

Purpose

Mammalian expression of full-length human DNAI1. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

DNAI1:M1-T699 (DNAI1 Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

A8S Y202C P495Q annotated sequence conflicts

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_DDB1:1-1140

Plasmid

#211188

Purpose

Mammalian expression of full-length human DDB1. loop deletion amino acids I396-D689. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

DDB1:M1-H1140 (DDB1 Human)

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

loop deletion I396-D689

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_CHAF1B:1-559

Plasmid

#211185

Purpose

Mammalian expression of full-length human CHAF1B. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

CHAF1B:M1-P559 (CHAF1B Human)

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBiT3.1-C_LRRK2:2142-2527

Plasmid

#211151

Purpose

Mammalian expression of human LRRK2 fragment R2142 to E2527. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

LRRK2:R2142-E2527 (LRRK2 Human)

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

M2397T natural variant ( VAR_024965)

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_FZR1:1-496

Plasmid

#211129

Purpose

Mammalian expression of full-length human FZR1. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

FZR1:M1-R496 (FZR1 Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-P1K-3VSV-Atg8(T56A R65A T87A)-MET15

Plasmid

#207051

Purpose

Expression of 3VSV-Atg8 point mutant. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).

Depositor

Zhiping Xie

Publication

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

ATG8

Tags

3xVSV

Expression

Yeast

Mutation

T56A R65A T87A

Available since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-P1K-3VSV-Atg8(R65A P85A P86A)-MET15

Plasmid

#207052

Purpose

Expression of 3VSV-Atg8 point mutant. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).

Depositor

Zhiping Xie

Publication

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

ATG8

Tags

3xVSV

Expression

Yeast

Mutation

R65A P85A P86A

Available since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-P1K-3VSV-Atg8(R65A P85L P86L)-MET15

Plasmid

#207053

Purpose

Expression of 3VSV-Atg8 point mutant. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).

Depositor

Zhiping Xie

Publication

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

ATG8

Tags

3xVSV

Expression

Yeast

Mutation

R65A P85L P86L

Available since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBiT3.1-C_EIF3B:186-685

Plasmid

#211279

Purpose

Mammalian expression of human EIF3B fragment V186 to T685. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

EIF3B:V186-T685 (EIF3B Human)

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBiT3.1-C_DCAF12:1-453

Plasmid

#211272

Purpose

Mammalian expression of full-length human DCAF12. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

DCAF12:M1-S453 (DCAF12 Human)

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

R131Q natural variant (VAR_035322)

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBiT3.1-N_DNAI4:1-848

Plasmid

#211258

Purpose

Mammalian expression of full-length human DNAI4. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

DNAI4:M1-A848 (WDR78 Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

G16A natural variant VAR_057635. C33W natural v…

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-myr-rsEGFP2- LDLR

Plasmid

#213399

Purpose

double floxed, myristoylation site (myr) and LDLR, fused to reversibly switchable EGFP rsEGFP2

Depositor

Katrin Willig

Publication

Willig et al Cell Rep. 2021 Jun 1;35(9):109192. doi:

Insert

rsEGFP2

Use

AAV

Tags

C-terminal (Ct) cytoplasmic domains of low densit…

Expression

Mammalian

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-h.Rhno1 T52A (B)-C-Myc-DDK-P2A-Puro

Plasmid

#211621

Purpose

Rhno1 T52A

Depositor

Agnel Sfeir

Publication

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

hRHNO1 (RHNO1 Human)

Tags

myc-flag

Expression

Mammalian

Mutation

T52A

Promoter

CMV

Available since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-h.Rhno1 S51A (A)-C-Myc-DDK-P2A-Puro

Plasmid

#211620

Purpose

Rhno1 S51A

Depositor

Agnel Sfeir

Publication

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

hRHNO1 (RHNO1 Human)

Tags

myc-flag

Expression

Mammalian

Mutation

S51A

Promoter

CMV

Available since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

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