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FES_HUMAN_D0

Plasmid

#79686

Purpose

This plasmid encodes the kinase domain of FES. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

FES (FES Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
MAPK3_HUMAN_D0

Plasmid

#79702

Purpose

This plasmid encodes the kinase domain of MAPK3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

MAPK3 (MAPK3 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pZS1[FruR-T,TreR-T]

Plasmid

#60772

Purpose

Contains PIq driving expression FruR-T, and PIq driving expression of TreR-T.

Depositor

Matthew Bennett

Article

Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

Inserts

FruR-T
TreR-T

Use

Synthetic Biology

Expression

Bacterial

Mutation

LacI/GalR repressor chimera. TAN DBD with FruR LB…

Available Since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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Flag-Src C185A H201A H492A E524A

Plasmid

#246427

Purpose

Expresses Src pH insensitive mutant in Mammalian cells

Depositor

Katharine White

Article

Van Dyck et al Sci Signal. 2025 Nov 11;18(912):eadt3018

Insert

Src (SRC Human)

Tags

Flag

Expression

Mammalian

Mutation

changed C188 to A, H204 to A, H495 to A, and E527…

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pUAS-spatzle-HA

Plasmid

#240235

Purpose

Expression of spatzle-HA under control of UAS promoter. Can be used to generate transgenic fly lines by white+ fly eye selection and phiC31 integration.

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv 2025.07.09.659702; doi: https://

Insert

spatzle (spz Fly)

Tags

HA-spGFP11

Expression

Insect

Promoter

UAS

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pTFG001-ACE2(WT)-HA

Plasmid

#191569

Purpose

ACE2 insert in a 2nd generation lentiviral transfer plasmid (pGIPZ) to generate ACE2(WT)-expressing cells. Codon-optimized for human expression. Insert contains C-terminal HA tag.

Depositor

Joshua Leonard

Article

Gunnels et al Small. 2022 May;18(19):e2200125. doi: 10

Insert

Angiotensin Converting Enzyme II (ACE2 Human)

Use

Lentiviral and RNAi

Tags

HA tag

Expression

Mammalian

Mutation

Codon optimized for expression in human cells

Promoter

CMV

Available Since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pMAL-MBP-FRB-KRas/N12

Plasmid

#214302

Purpose

Express split-KRas fragment fused with FRB and MBP

Depositor

Cliff Stains

Article

He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

Insert

Split-KRas fragment fused with FRB (KRAS Human)

Tags

Maltose-binding protein (MBP)

Expression

Bacterial

Promoter

Tac

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pET21b-KRas/13C-FKBP-MBP

Plasmid

#214303

Purpose

Express split-KRas fragment fused with FKBP and MBP

Depositor

Cliff Stains

Article

He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

Insert

Split-KRas fragment fused with FKBP (KRAS Human)

Tags

Maltose-binding protein (MBP)

Expression

Bacterial

Mutation

KRas-Q61L

Promoter

T7

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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Polymerase expression - pCMV-ePhi29(+exo) (LM2995)

Plasmid

#208966

Purpose

Unfused ePhi29 DNA polymerase (+exo), expressed from CMV or T7 promoters.

Depositor

Benjamin Kleinstiver

Article

Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

Insert

ePhi29(+exo)-BPNLS

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS

Expression

Mammalian

Mutation

ePhi29(+exo)(M8R/V51A/M97T/G197D/E221K/Q497P/K512…

Promoter

CMV and T7

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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Recruited polymerase expression - pCMV-T7-ePhi29-CC(N5) (LM2719)

Plasmid

#208973

Purpose

Recruited ePhi29 DNA polymerase (-exo), with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.

Depositor

Benjamin Kleinstiver

Article

Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

Insert

ePhi29-BPNLS-CC(N5)

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS

Expression

Mammalian

Mutation

ePhi29(-exo)(D169A;M8R/V51A/M97T/G197D/E221K/Q497…

Promoter

CMV and T7

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EFNB2-RBD-Fc (D62Q)

Plasmid

#200979

Purpose

Mammalian expression plasmid for soluble EFNB2 (D62Q specificity mutant) fused to IgG1 Fc

Depositor

Erik Procko

Article

Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

Insert

EFNB2 (EFNB2 Human)

Tags

IgG1 Fc

Expression

Mammalian

Mutation

Soluble Receptor-Binding Domain; D62Q mutation fo…

Promoter

CMV

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-EFNB2 (D62Q)

Plasmid

#200976

Purpose

Mammalian expression plasmid for myc-tagged EFNB2 (D62Q specificity mutant)

Depositor

Erik Procko

Article

Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

Insert

EFNB2 (EFNB2 Human)

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Mutation

D62Q (increases specificity towards henipaviral G…

Promoter

CMV

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-tetON-NKX2-1(delDBD)-EF1a-TagRFP-2A-tet3G

Plasmid

#204214

Purpose

Doxycycline inducible lentiviral vector for human NKX2-1 (delDBD) overexpression

Depositor

Emma Rawlins

Article

Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Deletion of DNA binding domain (delDBD)

Promoter

TetON

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-tetON-NKX2-1-R162del-EF1a-TagRFP-2A-tet3G

Plasmid

#204191

Purpose

Doxycycline inducible lentiviral vector for human NKX2-1 (R162del) overexpression

Depositor

Emma Rawlins

Article

Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Deletion of R162

Promoter

TetON

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-tetON-NKX2-1-I207M -EF1a-TagRFP-2A-tet3G

Plasmid

#204213

Purpose

Doxycycline inducible lentiviral vector for human NKX2-1 (I207M) overexpression

Depositor

Emma Rawlins

Article

Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Point mutation on I207M

Promoter

TetON

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-tetON-NKX2-1-Q175∗-EF1a-TagRFP-2A-tet3G

Plasmid

#204211

Purpose

Doxycycline inducible lentiviral vector for human NKX2-1 (Q175∗) overexpression

Depositor

Emma Rawlins

Article

Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Point mutation on Q175* (*termination)

Promoter

TetON

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-tetON-NKX2-1-R178∗-EF1a-TagRFP-2A-tet3G

Plasmid

#204210

Purpose

Doxycycline inducible lentiviral vector for human NKX2-1 (R178∗) overexpression

Depositor

Emma Rawlins

Article

Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Point mutation on R178* (*termination)

Promoter

TetON

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-tetON-NKX2-1-I207F -EF1a-TagRFP-2A-tet3G

Plasmid

#204212

Purpose

Doxycycline inducible lentiviral vector for human NKX2-1 (I207F) overexpression

Depositor

Emma Rawlins

Article

Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

Insert

NK2 homeobox 1 (NKX2-1 Human)

Use

Lentiviral

Tags

Point mutation on I207F

Expression

Mammalian

Promoter

TetON

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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mouse ZNG1

Plasmid

#197399

Purpose

expresses tagless mouse ZNG1 in the pHIS vector (inserted at NdeI-BamHI)

Depositor

David Giedroc

Article

Weiss et al Cell. 2022 Jun 9;185(12):2148-2163.e27.

Insert

ZNG1 (Cbwd1 Mouse)

Expression

Bacterial

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmThor-DmCup_417-1133_T

Plasmid

#147591

Purpose

Insect Expression of DmThor-DmCup_417-1133

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

DmThor-DmCup_417-1133 (Thor Fly)

Expression

Insect

Mutation

Thor contains two silent mutations A246C and T321…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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