We narrowed to 870 results for: Px330

1. pU6-(BsaI)_CBh-Cas9

   Plasmid

   #135010

   Purpose

   Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9

   Depositor

   Yosef Shaul

   Article

   Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio

   Insert

   humanized S. pyogenes Cas9
   

   Tags

   3xFLAG (N terminal on insert)

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. Human T (BRACHYURY) sgRNA

   Plasmid

   #59726

   Purpose

   Plasmid encoding sgRNA to generate T (BRACHYURY) knock-out mutant human cells

   Depositor

   Jacob Hanna

   Article

   Irie et al Cell. 2015 Jan 15;160(1-2):253-68. doi:

   Insert

   hT sgRNA (TBXT Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Dec. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pTij_sg_mmu_Med6_C_terminal

   Plasmid

   #197869

   Purpose

   CRISPR/Cas9/sgRNA plasmid for cutting Med6 C terminal and building MED6-mEmerald/Halo knock-in mESCs

   Depositor

   James Zhe Liu

   Article

   Dong et al bioRxiv 2022.10.26.513917

   Insert

   Med6 (Med6 Mouse)
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. qTAG-N-Solo-mStayGold-TUBA1B

   Plasmid

   #227327

   Purpose

   Donor template for mStayGold insertion into the N-terminus of the TUBA1B locus. For tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B (Addgene #207763)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   TUBA1B Homology Arms flanking a mStayGold Tag (TUBA1B Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Promoter

   Promoterless

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. qTAG-N-Solo-mScarlet-TUBA1B

   Plasmid

   #207764

   Purpose

   Donor template for mScarlet insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   TUBA1B Homology Arms flanking a mScarlet Tag (TUBA1B Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. qTAG-C-mScarlet-Puro-H2BC11

   Plasmid

   #207761

   Purpose

   Donor template for mScarlet-2A-Puro insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   H2BC11 Homology Arms flanking a mScarlet-Puro Cassette (H2BC11 Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Available Since

   Nov. 17, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. qTAG-C-moxGFP-Blast-H2BC11

   Plasmid

   #207757

   Purpose

   Donor template for moxGFP-2A-Blast insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   H2BC11 Homology Arms flanking a moxGFP-Blast Cassette (H2BC11 Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Available Since

   Nov. 16, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pMK312 (TOP2A CRISPR)

   Plasmid

   #140654

   Purpose

   TOP2A tagging CRISPR

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   TOP2A (TOP2A Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. SMC2 CRISPR

   Plasmid

   #140649

   Purpose

   SMC2 tagging CRISPR

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   SMC2 (SMC2 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. qTAG-C-mStayGold-Puro-PXN

    Plasmid

    #227317

    Purpose

    Donor template for mStayGold-2A-Puro insertion into the C-terminus of the PXN locus. For focal adhesion visualization. To be co-transfected with sgRNA plasmid px330-PITCh-PXN (Addgene #227315)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    PXN Homology Arms flanking a mStayGold-2A-Puro Cassette (PXN Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Promoter

    Promoterless

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. qTAG-C-mStayGold-Puro-H2BC11

    Plasmid

    #227332

    Purpose

    Donor template for mStayGold-2A-Puro insertion into the C-terminus of the H2BC11 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 (Addgene #207755)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    H2BC11 Homology Arms flanking a mStayGold-2A-Puro Cassette (H2BC11 Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Promoter

    Promoterless

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. Cas9-sgRNA-A+B

    Plasmid

    #149370

    Purpose

    Expresses SpCas9 and two sgRNA targeting the lenti-CDDR reporter at two distal sites

    Depositor

    Tarek Abbas

    Article

    Eki et al Nucleic Acids Res. 2020 Oct 17. pii: 592

    Inserts

    Cas9
    sgRNA-A
    sgRNA-B
    Puromycin resistance
    

    Use

    CRISPR

    Tags

    3xFLAG, Nucleoplasmin NLS, and SV40 NLS

    Expression

    Mammalian

    Promoter

    CBh, PGK, and U6

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. qTAG-C-moxGFP-Puro-H2BC11

    Plasmid

    #207758

    Purpose

    Donor template for moxGFP-2A-Puro insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    H2BC11 Homology Arms flanking a moxGFP-Puro Cassette (H2BC11 Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Available Since

    Nov. 17, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. qTAG-C-miRFP670nano3-Blast-H2BC11

    Plasmid

    #207762

    Purpose

    Donor template for miRFP670nano3-2A-Blast insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    H2BC11 Homology Arms flanking a miRFP670nano3-Blast Cassette (H2BC11 Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Available Since

    Nov. 29, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. qTAG-C-moxGFP-Zeo-H2BC11

    Plasmid

    #207759

    Purpose

    Donor template for moxGFP-2A-Zeo insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    H2BC11 Homology Arms flanking a moxGFP-Zeo Cassette (H2BC11 Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Available Since

    Nov. 17, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. qTAG-N-Solo-mStayGold-MYO1C

    Plasmid

    #227306

    Purpose

    Donor template for mStayGold insertion into the N-terminus of the MYO1C locus. For membrane visualization. To be co-transfected with sgRNA plasmid px330-PITCh-MYO1C (Addgene #227305)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    MYO1C Homology Arms flanking a mStayGold Tag (MYO1C Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Promoter

    Promoterless

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTUB1-Spy.dCas9-CAT-U6-sgRNA(decoy)

    Plasmid

    #171089

    Purpose

    CRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus pyogenes in Toxoplasma gondii. Suitable for generating stable cell lines.

    Depositor

    Sebastian Lourido

    Article

    Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1

    Inserts

    Decoy sgRNA
    dCas9
    Chloramphenicol acetyltransferase
    

    Use

    CRISPR; Toxoplasma gondii expression

    Tags

    3X FLAG and Nuclear Localization Signal

    Mutation

    D10A, H840A

    Promoter

    TUB1 (Toxoplasma gondii) and U6 (Toxoplasma gondi…

    Available Since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pgRGFP

    Plasmid

    #82695

    Purpose

    Expresses gRNA of interest under U6 promoter (cloning by BpiI) and GFP under PGK promoter.

    Depositor

    Alan Mullen

    Article

    Daneshvar et al Cell Rep. 2016 Oct 4;17(2):353-365. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    PGK, U6

    Available Since

    Nov. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pYL214

    Plasmid

    #171031

    Purpose

    CRISPR plasmids encodes Cas9 and a sgRNA targeting EZH2 exon 2 - intron 2 junction (sequence: GCAGACGAGCTGATGAAGTAA)

    Depositor

    Thomas Cech

    Article

    Long et al Nat Genet. 2020 Sep;52(9):931-938. doi:

    Insert

    EZH2 (EZH2 Human)
    

    Use

    CRISPR

    Available Since

    Aug. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. qTAG-C-mNeon-Blast-TUBB4B

    Plasmid

    #207786

    Purpose

    Donor template for mNeon-2A-Blast insertion into the C-terminus of the TUBB4B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBB4B Addgene #207785

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    TUBB4B Homology Arms flanking a mNeon-Blast Cassette (TUBB4B Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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