We narrowed to 4,636 results for: pcr

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pCri-16a

Plasmid

#61324

Purpose

Protein expression.

Depositor

F Xavier Gomis-Ruth

Article

Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

Insert

Green fluorescent protein

Tags

His-tag

Expression

Bacterial

Available Since

Jan. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pCri-14a

Plasmid

#61322

Purpose

Protein expression.

Depositor

F Xavier Gomis-Ruth

Article

Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

Insert

Green fluorescent protein

Tags

His-tag and LSL-tag

Expression

Bacterial

Available Since

Jan. 9, 2015

Availability

Academic Institutions and Nonprofits only
pCri-9a-Strep

Plasmid

#61341

Purpose

Protein expression.

Depositor

F Xavier Gomis-Ruth

Article

Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

Insert

Green fluorescent protein

Tags

Strep-tag

Expression

Bacterial

Available Since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only
pCri-8a-Strep

Plasmid

#61340

Purpose

Protein expression.

Depositor

F Xavier Gomis-Ruth

Article

Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

Insert

Green fluorescent protein

Tags

His-tag and Strep-tag

Expression

Bacterial

Available Since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only
pCR4-*mRFP

Plasmid

#47118

Purpose

Can be used to make an RNA probe for whole-mount in situ hybridization, for in-frame fusion proteins or as a subcloning step for mRFP. Note that the coding sequence for mRFP lacks the start codon.

Depositor

Stephen Ekker

Article

Clark et al Nat Methods. 2011 Jun;8(6):506-15. Epub

Insert

*mRFP

Expression

Bacterial

Mutation

No start codon

Promoter

Lac

Available Since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only

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pCR2.1-ZFpparg

Plasmid

#44151

Purpose

This is intended for use in riboprobe synthesis, not for cloning.

Depositor

John Rawls

Article

Flynn EJ et al J Lipid Res. 2009 Aug;50(8):1641-52. doi

Insert

Peroxisome proliferator-activated receptor gamma (pparg Zebrafish)

Available Since

Aug. 16, 2013

Availability

Academic Institutions and Nonprofits only
pCRII-SNORD115-11

Plasmid

#45890

Depositor

Stefan Stamm

Article

Falaleeva et al Clin Med Insights Case Rep. 2013 May 5;6

Insert

SNORD115 without flanking exons and intron (SNORD115-11 Human)

Use

Unspecified

Available Since

Aug. 5, 2013

Availability

Academic Institutions and Nonprofits only
nanos-PCRII

Plasmid

#26601

Depositor

Phillip Newmark

Article

Wang et al Proc Natl Acad Sci U S A. 2007 Apr 3. 10

Insert

nanos

Available Since

Oct. 28, 2010

Availability

Academic Institutions and Nonprofits only
PCRII-zSynC

Plasmid

#20123

Depositor

Aaron Gitler

Insert

zSynC (sncb Zebrafish)

Use

In-situ

Available Since

Jan. 20, 2009

Availability

Academic Institutions and Nonprofits only
pCRISPaint-T2A-Gal80-3xP3-RFP

Plasmid

#127562

Purpose

CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Gal80-SV40 and 3xP3-RFP visible eye marker.

Depositor

Norbert Perrimon

Article

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

Gal80

Use

CRISPR

Expression

Insect

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-T2A-Gal4DBD-3xP3-RFP

Plasmid

#127564

Purpose

CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Gal4DBD-SV40 and 3xP3-RFP visible eye marker.

Depositor

Norbert Perrimon

Article

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

Gal4DBD

Use

CRISPR

Expression

Insect

Available Since

July 13, 2019

Availability

Academic Institutions and Nonprofits only

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pCRISPEY-GAL-Nat

Plasmid

#232097

Purpose

Yeast CEN plasmid for galactose-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with NatMX selection

Depositor

Jeffrey Lewis

Article

Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pCRISPR-cBEST-v2-PermE

Plasmid

#209446

Purpose

improved pCRISPR-cBEST plasmid with optimized sgRNA cloning site

Depositor

Tilmann Weber

Article

Maleckis et al ACS Chem Biol. 2024 Feb 10. doi: 10.1021

Insert

Codon optimized APOBEC1-nCas9-UGI fusion protein

Use

CRISPR and Synthetic Biology

Promoter

PtipA; sgRNA expression from ermE* promoter

Available Since

March 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCRISPR Human CASP9 -2

Plasmid

#198420

Purpose

Expresses Human CASP9 Specific gRNA/Cas9 Complex and Reporter Protein

Depositor

Hannah Rabinowich

Article

Han et al Autophagy. 2021 Jun;17(6):1393-1409. doi

Insert

Human CASP9 Specific gRNA (CASP9 Human)

Use

CRISPR

Tags

Cas9/Orange Fluorescent Protein Reporter

Expression

Mammalian

Promoter

U6; CMV

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCRISPR Human CASP9 -1

Plasmid

#198419

Purpose

Expresses Human CASP9 Specific gRNA/Cas9 Complex and Reporter Protein

Depositor

Hannah Rabinowich

Article

Han et al Autophagy. 2021 Jun;17(6):1393-1409. doi

Insert

Human CASP9 Specific gRNA (CASP9 Human)

Use

CRISPR

Tags

Cas9/Orange Fluorescent Protein Reporter

Expression

Mammalian

Promoter

U6; CMV

Available Since

April 13, 2023

Availability

Academic Institutions and Nonprofits only

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AAV.pCry1(408).DIO.Luciferase

Plasmid

#192623

Purpose

Cre-dependent expression of firefly luciferase, driven by a minimal mouse Cry1 promoter

Depositor

Michael Hastings

Article

Brancaccio et al Science. 2019 Jan 11;363(6423):187-192.

Insert

luciferase

Use

AAV

Expression

Mammalian

Available Since

Nov. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCRB DRT7VTPLux*500

Plasmid

#107959

Purpose

Controller plasmid [ca. 558.5 relative RBS strength at PLas81*]

Depositor

Jim Haseloff

Article

Boehm et al Nat Commun. 2018 Feb 22;9(1):776. doi: 1

Insert

T7RNAP (modified)

Use

Synthetic Biology

Expression

Bacterial

Available Since

April 9, 2018

Availability

Academic Institutions and Nonprofits only

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pCR8/GW/TOPO-ELK1

Plasmid

#98616

Purpose

Gateway entry TOPO TA cloning vector for ELK1

Depositor

Richard Harvey

Article

Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

Insert

ELK1 (Elk1 Mouse)

Use

Topo ta cloning vector

Mutation

Q230R

Available Since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pCR8/GW/TOPO-ELK4

Plasmid

#98617

Purpose

Gateway entry TOPO TA cloning vector for ELK4

Depositor

Richard Harvey

Article

Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

Insert

ELK4 (Elk4 Mouse)

Use

Topo ta cloning vector

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-StrepTag II-PuroR

Plasmid

#80967

Purpose

CRISPaint gene tagging

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

StrepTag II

Use

Gene tagging

Promoter

none

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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