We narrowed to 5,623 results for: crispr cas9 grna plasmid

1. pUC119-gRNA

   Plasmid

   #52255

   Purpose

   Can use a PCR template to assemble new desired guide RNA. Contains an Arabidopsis U6 promoter to drive guide RNA (targeting AtPDS3 gene target site 1) expression with a TTTTTT as terminator.

   Depositor

   Jen Sheen

   Article

   Li et al Nat Biotechnol. 2013 Aug;31(8):688-91. d

   Insert

   guide RNA targeting AtPDS3 (PDS3 Mustard Weed)
   

   Use

   CRISPR; Plant expression

   Tags

   Expression

   Mutation

   Promoter

   Arabidopsis U6 polymerase III promoter

   Available since

   March 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSpCAS9n(BB)-2A-puro DNM1, A

   Plasmid

   #107795

   Purpose

   gRNA vector for knock-in gene editing at human DNM1 locus

   Depositor

   Sandra Schmid

   Article

   Srinivasan et al PLoS Biol. 2018 Apr 18;16(4):e2005377. d

   Insert

   gRNA targeting human DNM1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSpCAS9n(BB)-2A-puro DNM1, B

   Plasmid

   #107796

   Purpose

   gRNA vector for knock-in gene editing at human DNM1 locus

   Depositor

   Sandra Schmid

   Article

   Srinivasan et al PLoS Biol. 2018 Apr 18;16(4):e2005377. d

   Insert

   gRNA targeting human DNM1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSPCas9(BB)-2A-GFP_ABCA3GFP_targeting

   Plasmid

   #188541

   Purpose

   Plasmid encoding pCas9 and gRNA targeting the endogenous human ABCA3 locus stop codon

   Depositor

   Darrell Kotton

   Article

   Sun et al Am J Respir Cell Mol Biol. 2021 Oct;65(4

   Insert

   gRNA
   

   Use

   CRISPR

   Tags

   GFP

   Expression

   Mutation

   Promoter

   U6

   Available since

   Sept. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSMART-sgRNA (Sp)

   Plasmid

   #80427

   Purpose

   U6 promoter driven sgRNA expression plasmid. Annealed oligonucleotides encoding crRNA sequence are ligated into BbsI site.

   Depositor

   Melissa Little, Sara Howden

   Article

   Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. AAT_g1 gRNA

   Plasmid

   #86005

   Purpose

   plasmid vector encoding for U6-driven AAT_g1 gRNA

   Depositor

   Tobias Cantz

   Article

   Eggenschwiler et al Sci Rep. 2016 Dec 2;6:38198. doi: 10.103

   Insert

   pU6-AAT_g1 sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pU6

   Available since

   Feb. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. AAT_g2 gRNA

   Plasmid

   #86006

   Purpose

   plasmid vector encoding for U6-driven AAT_g2 gRNA (Z-allele specific)

   Depositor

   Tobias Cantz

   Article

   Eggenschwiler et al Sci Rep. 2016 Dec 2;6:38198. doi: 10.103

   Insert

   pU6-AAT_g2 sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSTBlue-U6-sgRNA(tdTomato)

   Plasmid

   #47871

   Purpose

   This plasmid contains expression cassette for sgRNA targeting tdTomato.

   Depositor

   Erik Sontheimer, James Thomson

   Article

   Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.

   Insert

   sgRNA-tdTomato
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCAS9counter

   Plasmid

   #107192

   Purpose

   Expresses CAS9 and sgRNA for targeted counterselection in S. aureus

   Depositor

   Steve Salipante

   Article

   Penewit et al MBio. 2018 Feb 20;9(1). pii: mBio.00067-

   Inserts

   Cas9
   sgRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   prab17

   Available since

   Feb. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. EXOSC10 gRNA (BRDN0001148841)

    Plasmid

    #77989

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human EXOSC10

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    EXOSC10 (EXOSC10 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge