We narrowed to 14,439 results for: NTS

1. pLVX-Puro-TDP-43-A382T

   Plasmid

   #133756

   Purpose

   Lentiviral expression of human TDP-43 A382T disease mutant in mammalian cells.

   Depositor

   Shawn Ferguson

   Article

   Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Lentiviral

   Mutation

   Changed alanine 382 to threonine

   Promoter

   CMV

   Available Since

   Dec. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMOD_B2112

   Plasmid

   #91064

   Purpose

   Module B, Promoter: PvUbi1, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers , Terminator: 35S

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers
   

   Use

   CRISPR

   Promoter

   PvUbi1

   Available Since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. 1051 pBabe puroL Akt

   Plasmid

   #9011

   Depositor

   William Sellers

   Article

   DiRenzo et al Cancer Res. 2002 Jan 1. 62(1):89-98.

   Insert

   Akt1 (AKT1 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Nov. 14, 2005

   Availability

   Academic Institutions and Nonprofits only

4. pMOD_B2203

   Plasmid

   #91066

   Purpose

   Module B, Promoter: CmYLCV, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers (only works with pMOD_C2200), Terminator: none - to be fused to gRNA array in MODULE C

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers
   

   Use

   CRISPR

   Promoter

   CmYLCV

   Available Since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. tdp43-EGFP construct2

   Plasmid

   #28195

   Depositor

   Zuoshang Xu

   Article

   Yang et al PLoS One. 2010 . 5(12):e15878.

   Insert

   Tar DNA binding protein (TARDBP Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   aa86-414 only

   Available Since

   Sept. 8, 2011

   Availability

   Academic Institutions and Nonprofits only

6. pAT9752-BEAR-mScarlet

   Plasmid

   #162991

   Purpose

   BEAR target plasmid with split mScarlet and disrupted 5' splice site

   Depositor

   Ervin Welker

   Article

   Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

   Insert

   mScarlet split with an intron between amino acids 110-111
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   disrupted 5' splice site

   Promoter

   CMV

   Available Since

   Sept. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLX-Z4 (GB3375)

   Plasmid

   #160648

   Purpose

   pLX series: RK2-based T-DNA binary vector for plant transformation (alpha1)

   Depositor

   Juan Antonio García, Fabio Pasin

   Article

   Pasin et al ACS Synth Biol. 2017 Oct 20;6(10):1962-1

   Type

   Empty backbone

   Use

   Synthetic Biology

   Available Since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPPEDs

   Plasmid

   #162469

   Purpose

   protoplast test vector of pPPED

   Depositor

   Adam Bogdanove

   Article

   Wang et al Front Genome Ed. 2021 Mar 4;3:617553. do

   Inserts

   Cas9H840A
   attR gateway destination cassette
   

   Use

   Prime editing destination vector for transient ex…

   Tags

   MLV reverse transcriptase

   Expression

   Plant

   Mutation

   Change histidine 840 to Alanine

   Available Since

   Dec. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYPQ-SpRY-Act3.0

   Plasmid

   #178957

   Purpose

   It consists of a catalytically active SpRY nuclease and MS2-SunTag-activators activation complex (ScFv-sfGFP-2xTAD), enabling simultaneous genome editing and gene activation.

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2022 May;8(5):513-525. doi:

   Insert

   SpRY-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAsCpf1(TATV)(BB) (pY221)

    Plasmid

    #89354

    Purpose

    Expresses humanized AsCpf1 TATV PAM variant and crRNA guide

    Depositor

    Feng Zhang

    Article

    Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

    Insert

    Cpf1 RVR variant
    

    Use

    CRISPR

    Tags

    NLS and NLS-3xHA

    Expression

    Mammalian

    Mutation

    S542R/K548V/N552R

    Promoter

    CBh

    Available Since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMOD_B2103b

    Plasmid

    #91062

    Purpose

    Module B, Promoter: CmYLCV, Gene: SapI ccdb cassette (doesn't include the promoter) for cloning multiple gRNA spacers with Csy4 spacers , Terminator: 35S

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    SapI ccdb cassette (no promoter) for cloning multiple gRNA spacers with Csy4 spacers
    

    Use

    CRISPR

    Promoter

    CmYLCV

    Available Since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. dxCas(3.6)-VPR

    Plasmid

    #108388

    Purpose

    Mammalian expression vector for xCas9 3.6 with VP64-p65-Rta (VPR) fused to its C-terminus

    Depositor

    David Liu

    Article

    Hu et al Nature. 2018 Feb 28. pii: nature26155. d

    Insert

    dxCas(3.6)-VPR
    

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    E108G, S217A, A262T, S409I, E480K, E543D, M694I, …

    Available Since

    March 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDNA3 Flag MKK3b(Glu)

    Plasmid

    #50449

    Purpose

    activates p38 in the signaling pathway

    Depositor

    Roger Davis

    Article

    Enslen et al EMBO J. 2000 Mar 15. 19(6):1301-11.

    Insert

    MKK3b (MAP2K3 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    changed Serine 218 to Glutamic Acid, changed Thre…

    Promoter

    CMV

    Available Since

    Jan. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

14. tdp43-EGFP construct5

    Plasmid

    #28198

    Depositor

    Zuoshang Xu

    Article

    Yang et al PLoS One. 2010 . 5(12):e15878.

    Insert

    Tar DNA binding protein (TARDBP Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    aa257-414 only

    Available Since

    Sept. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

15. Sniper-Cas9

    Plasmid

    #138559

    Purpose

    Expresses human codon-optimized Sniper-SpCas9 and blasticidin resistance: EFS promoter-Sniper-Cas9-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

    Insert

    Sniper-Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    F539S, M763I, K890N

    Promoter

    EFS

    Available Since

    June 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. AM77_pU6.Sa-gRNA.CLYBL

    Plasmid

    #199237

    Purpose

    Expression construct encoding a S. aureus guide RNA targeting the human CLYBL safe harbor locus

    Depositor

    Manuel Goncalves

    Article

    Wang et al Nucleic Acids Res. 2023 Mar 17:gkad165.

    Insert

    S. aureus gRNA spacer
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    RNA polymerase III promoter for human U6 snRNA

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. SPLINTR mCHERRY v1 backbone

    Plasmid

    #179778

    Purpose

    SPLINTR backbone vector

    Depositor

    Mark Dawson

    Article

    Fennell et al Nature. 2022 Jan;601(7891):125-131. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pQE80L-SH3

    Plasmid

    #160331

    Purpose

    This plasmid encodes an engineered oxygen-independent flavin binding fluorescent protein with N-terminal 6xHis tag. Its thermostability and quantum yield is improved compared to wild type.

    Depositor

    Sang Taek Jung

    Article

    Ko et al J Agric Food Chem. 2019 Oct 30;67(43):12

    Insert

    SH3
    

    Tags

    6xHis tag

    Expression

    Bacterial

    Promoter

    T5

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCW57-CMV-MAAP-SL08

    Plasmid

    #218247

    Purpose

    1) Ectopic expression of MAAP protein variant in mammalian cells. 2) Packaging MAAP variant sequence into lentivirus for stable expression in mammalian cells.

    Depositor

    David Schaffer

    Article

    Schieferecke et al Mol Ther. 2024 Feb 7;32(2):340-351. doi:

    Insert

    Membrane-Associated Accessory Protein Variant SL08 driven by CMV promoter
    

    Use

    Lentiviral

    Promoter

    CMV

    Available Since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG/hTAZ-FL

    Plasmid

    #139979

    Purpose

    Expresses full length human Tafazzin with codon modified for cloning. Transgene flanked by AAV2 ITR

    Depositor

    William Pu

    Article

    Wang et al Circ Res. 2020 Mar 9. doi: 10.1161/CIRCR

    Insert

    Full length human Tafazzin (TAFAZZIN Human)
    

    Use

    AAV

    Promoter

    CAG

    Available Since

    April 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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