We narrowed to 14,439 results for: NTS

1. pJ3 SHP2 D61A

   Plasmid

   #8320

   Depositor

   Ben Neel

   Article

   Bennett et al Mol Cell Biol 1996 Mar;16(3):1189-202.

   Insert

   SHP2 (PTPN11 Human)
   

   Expression

   Mammalian

   Mutation

   D61A (activated mutant)

   Available Since

   June 17, 2005

   Availability

   Academic Institutions and Nonprofits only

2. pX330.Pten.b

   Plasmid

   #66588

   Purpose

   sgRNA for Pten deletion

   Depositor

   Wen Xue

   Article

   Li et al Genome Biol. 2015 May 28;16(1):111.

   Insert

   Pten deletion (Pten Mouse)
   

   Expression

   Mammalian

   Available Since

   July 27, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pEB2_mScarletI_mVenusNB-MrkHR113A

   Plasmid

   #182292

   Purpose

   expresses c-di-GMP mutant biosensor in E. coli

   Depositor

   Howard Berg

   Article

   Vrabioiu et al Proc Natl Acad Sci U S A. 2022 Feb 8;119

   Insert

   mVenusNB-MrkHR113A
   

   Tags

   mVenusNB internal fusion to MrkH with a R113A poi…

   Expression

   Bacterial

   Mutation

   changed arginine 113 to alanine, MrkH numbering …

   Promoter

   proC

   Available Since

   March 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBabe puro H-Ras G12V T35S

   Plasmid

   #12274

   Depositor

   Bob Weinberg

   Article

   Rangarajan et al Cancer Cell. 2004 Aug . 6(2):171-83.

   Insert

   H-Ras G12V T35S (HRAS Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   Contains G12V activating mutation. The T35S mutat…

   Available Since

   July 20, 2006

   Availability

   Academic Institutions and Nonprofits only

5. PCRT7 tetR pLtetO MBP-MEK1 XX Amp

   Plasmid

   #53225

   Purpose

   human MEK1 with MBP and HIS tags, with Ser218 and Ser222 changed to amber codons, for use in phosphoprotein synthesis

   Depositor

   Jesse Rinehart

   Insert

   MEK1 (MAP2K1 Human)
   

   Use

   Phosphoprotein synthesis

   Tags

   HIS and MBP

   Expression

   Bacterial

   Mutation

   Codon optimized for E.coli, both codons for Ser21…

   Promoter

   pLtetO

   Available Since

   May 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pES4/4a

   Plasmid

   #69590

   Purpose

   TCR a chain cDNA in pES4 transgenic construct for creating OT-II mice

   Depositor

   Francis R. Carbone

   Article

   Barnden et al Immunol Cell Biol. 1998 Feb;76(1):34-40.

   Insert

   TCR alpha (Tcra Mouse)
   

   Expression

   Mammalian

   Promoter

   H-2Kb

   Available Since

   Sept. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

7. CABE-T2.19

   Plasmid

   #193265

   Purpose

   Expression of CABE-T2.19 base editor in mammalian cells, CMV promoter

   Depositor

   Nicole Gaudelli

   Article

   Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

   Insert

   CABE-T2.19
   

   Expression

   Mammalian

   Mutation

   D10A (Cas9), TadA mutations within

   Promoter

   CMV

   Available Since

   Feb. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFRT/FLAG/HA-DEST LIN28A

   Plasmid

   #43797

   Depositor

   Thomas Tuschl

   Article

   Hafner et al RNA. 2013 May;19(5):613-26. doi: 10.1261

   Insert

   LIN28A (LIN28A Human)
   

   Tags

   FLAG-HA

   Expression

   Mammalian

   Available Since

   April 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. Venus-Noxa-pEGFP-C1

   Plasmid

   #166738

   Purpose

   Express Venus fused to the N-terminus of the Bcl-2 family protein, Noxa

   Depositor

   David Andrews

   Article

   Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

   Insert

   Noxa (PMAIP1 Human)
   

   Tags

   Venus

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. p2T-CAG-KKHSaCas9-BlastR

    Plasmid

    #107189

    Purpose

    Confers constitutive expression of KKH SaCas9.

    Depositor

    Richard Sherwood

    Article

    Shen et al Nature. 2018 Nov;563(7733):646-651. doi:

    Insert

    KKH SaCas9 (NEWENTRY )
    

    Expression

    Bacterial

    Mutation

    E782K/N968K/R1015H

    Promoter

    Chicken ß-Actin

    Available Since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCD564

    Plasmid

    #153026

    Purpose

    dxCas9(3.7), MCP-SoxS(R93A/S101A)

    Depositor

    Jesse Zalatan

    Article

    Fontana et al Nat Commun. 2020 Apr 1;11(1):1618. doi:

    Insert

    dxCas9(3.7), MCP-SoxS(R93A/S101A)
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    SoxS has R93A and S101A mutations

    Available Since

    Aug. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRK5RS-HERCD533

    Plasmid

    #65224

    Purpose

    Expression of dominant negative EGFR in mammalian cells

    Depositor

    Axel Ullrich

    Article

    Redemann et al Mol Cell Biol. 1992 Feb;12(2):491-8.

    Insert

    HERCD533 (EGFR Human)
    

    Expression

    Mammalian

    Mutation

    deleted AA 654-1186

    Promoter

    CMV

    Available Since

    Dec. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

13. CoV2-N-R203K

    Plasmid

    #177951

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (R203K). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (R203K) (N )
    

    Expression

    Mammalian

    Mutation

    R203K

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA5-FRT/TO-N-mEGFP-PARP6

    Plasmid

    #178005

    Purpose

    Expresses N-terminally mEGFP-tagged PARP6

    Depositor

    Karla Feijs

    Article

    Weixler et al Life Sci Alliance. 2022 Nov 11;6(1):e202

    Insert

    PARP6 (PARP6 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA5-FRT/TO-N-mEGFP-PARP15

    Plasmid

    #178000

    Purpose

    Expresses N-terminally mEGFP-tagged PARP15

    Depositor

    Karla Feijs

    Article

    Weixler et al Life Sci Alliance. 2022 Nov 11;6(1):e202

    Insert

    PARP15 (PARP15 Human)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFRT/FLAG/HA-DEST LIN28B

    Plasmid

    #43798

    Depositor

    Thomas Tuschl

    Article

    Hafner et al RNA. 2013 May;19(5):613-26. doi: 10.1261

    Insert

    LIN28B (LIN28B Human)
    

    Tags

    FLAG-HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAGGS-Halotag-loxP-P2A-dTKneo-pA-loxP

    Plasmid

    #139485

    Purpose

    PCR template for Halo-Tag gene knock-in donor vector

    Depositor

    Kazuhiro Aoki

    Article

    Komatsubara et al J Biol Chem. 2019 Apr 12;294(15):6062-60

    Insert

    HaloTag-loxP-P2A-dTKneo-pA-loxP
    

    Tags

    HaloTag

    Expression

    Mammalian

    Promoter

    CAG promoter

    Available Since

    April 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYPQ292 (AaCas12b)-D570A-TV-MS2-VPR

    Plasmid

    #136382

    Purpose

    CRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation system, followed by T2A and MCP-VPR fusion protein

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Insert

    AaCas12b (D570A)-TV-T2A-MCP-VPR
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    D570A

    Available Since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCEP4-myc-HLA-B8

    Plasmid

    #135512

    Purpose

    Mammalian expression of myc-tagged HLA-B*08:01

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    HLA-B*08:01 (HLA-B Human)
    

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. SPLINTR GFP v1 backbone

    Plasmid

    #179777

    Purpose

    SPLINTR backbone vector

    Depositor

    Mark Dawson

    Article

    Fennell et al Nature. 2022 Jan;601(7891):125-131. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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