We narrowed to 146,921 results for: ins;

1. pOmnibac_ZZ_linker_K963_GFP_SNAP

   Plasmid

   #159727

   Purpose

   Expresses kinesin-1 (amino acids 1-963) in Sf9 cells with a C-terminal GFP and SNAP tag

   Depositor

   Ahmet Yildiz

   Article

   Ferro et al Science. 2022 Jan 21;375(6578):326-331.

   Insert

   KIF5B (full length, 1-963 amino acids), K963 (KIF5B Human)
   

   Use

   Tags

   GFP and SNAP tags and ZZ affinity tag

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Jan. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. TDP-REGv2 Cryptic PE-Max

   Plasmid

   #216163

   Purpose

   Expression of prime editor PEMax (Plasmid #174820) only in cells with TDP-43 loss of function. Note: It is not recommended to produce lentivirus containing TDP-REG sequences – see Depositor Comments

   Depositor

   Pietro Fratta

   Article

   Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi

   Insert

   PEMax for prime editing with internal cryptic exon
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. TH1204-pOCC120-FUS_opt(NheI)

   Plasmid

   #221892

   Purpose

   Shuttle vector for baculovirus production, using FlashBac bacmid

   Depositor

   Anthony Hyman

   Article

   Wang et al Cell. 2018 Jul 26;174(3):688-699.e16. do

   Insert

   FUS (FUS Human)
   

   Use

   Tags

   MBP-PreScission and mGFP-PreScission-6xHis

   Expression

   Insect

   Mutation

   wt

   Promoter

   PH promoter

   Available Since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR221-SLC45A2_STOP

   Plasmid

   #161420

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC45A2 (SLC45A2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 15, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pDONR221-SLC43A1_STOP

   Plasmid

   #161344

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC43A1 (SLC43A1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 15, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pDONR221-SLC10A2_STOP

   Plasmid

   #161085

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC10A2 (SLC10A2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 21, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pDONR221-SLC7A2_STOP

   Plasmid

   #161103

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC7A2 (SLC7A2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 21, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pDONR221-SLC17A9_STOP

   Plasmid

   #161139

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC17A9 (SLC17A9 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 21, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pDONR221-SLC22A3_STOP

   Plasmid

   #161190

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC22A3 (SLC22A3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 21, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-T4DNApol (JO638)

    Plasmid

    #208956

    Purpose

    A variant CE1 construct with T4 DNA polymerase (-exo, activity enhanced), expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-XTEN-nSpCas9-BPNLS-T4pol-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A), T4Pol(-exo;D189A/E191A/L412M)

    Promoter

    CMV and T7

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDONR221-SLC7A3_STOP

    Plasmid

    #161467

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC7A3 (SLC7A3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 15, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pDONR221-SLC27A4_STOP

    Plasmid

    #161449

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC27A4 (SLC27A4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 15, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pCMVtrunc sfCherry2ΔC4-Lifeact

    Plasmid

    #231557

    Purpose

    Mammalian expression of Lifeact fused to C-terminally truncated superfolder Cherry2, for actin filament organization measurements using fluorescence polarization microscopy

    Depositor

    Manos Mavrakis

    Article

    Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

    Insert

    Lifeact (ABP140 Budding Yeast)
    

    Use

    Tags

    sfCherry2

    Expression

    Mammalian

    Mutation

    Promoter

    CMVtrunc

    Available Since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDONR221-SLC24A2_STOP

    Plasmid

    #161132

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC24A2 (SLC24A2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 21, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pDONR221-SLC43A2_STOP

    Plasmid

    #161356

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC43A2 (SLC43A2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 15, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pDONR221-SLC9A6_STOP

    Plasmid

    #161341

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC9A6 (SLC9A6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 15, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. HUHe variant click editor (CE1) - pCMV-T7-DCV-nCas9-EcKlenow (JO608)

    Plasmid

    #208946

    Purpose

    A variant CE1 construct with DCV HUH endonuclease, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    DCV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available Since

    Nov. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1-N-Myc_535-888 USP7

    Plasmid

    #131248

    Purpose

    Mammalian expression of the USP7 UBL1-3 domains with an N-terminal Myc tag.

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    A mutant form of our pcDNA3.1-N-Myc_535-1102 USP7…

    Promoter

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pmCherry-imp α

    Plasmid

    #119719

    Purpose

    Expresses mCherry-tagged human importinα in mammalian cells

    Depositor

    Kenji Sugimoto

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2012;76(2):38

    Insert

    importinα (KPNA2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    contains amino acids 251-529

    Promoter

    CMV

    Available Since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-Phi29(+exo) (LM2938)

    Plasmid

    #208957

    Purpose

    A variant CE1 construct with Phi29 DNA polymerase (exo active), expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-XTEN-nSpCas9-BPNLS-Phi29(+exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A)

    Promoter

    CMV and T7

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge