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Plasmid#158663PurposeTet-OsTIR1(WT)-V5 donor for integration at the AAVS1 locusDepositorInsertTet-OsTIR1(WT) AAVS1
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
p59sheLL
Plasmid#40628DepositorInsertsHPV59 L1
HPV59 L2
UseTagsExpressionMammalianMutationCodon optimized for mammalian expressionPromoterCMV and Polio IRESAvailable sinceOct. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDipA-TEV-His12
Plasmid#137042Purposeexpression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi DipA with C-terminal TEV-protease-cleavable His12DepositorInsertAAC66790.1 (BB_0418 Borrelia burgdorferi B31)
UseTagsHis12 (TEV protease cleavable)ExpressionBacterialMutationwild type, full-length protein, contains signal p…PromoterT7Available sinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMTS_mScarlet_N1
Plasmid#85057PurposeIn vivo visualization of the mitochondria (can be used for colocalization studies)DepositorInsertMTS (COX8A Human)
UseTagsmScarletExpressionMammalianMutationaa1-29PromoterCMVAvailable sinceMarch 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_eCBmut
Plasmid#164605PurposeExpress the endocannabinoid non-responsive sensor GRAB_eCBmut in neuronsDepositorInsertEndocannabinoid non-responsive sensor GRAB_eCBmut
UseAAVTagsExpressionMutationS383T, F177APromoterhSynAvailable sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHSN6I01
Plasmid#50587PurposeCRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-KRAB, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistanceDepositorInsertsdCas9-KRAB
gRNA scaffold
UseCRISPR; Plant expressionTags3xFLAG, HA, and NLSExpressionMutationdCas9 that is defective in DNA cleavage; the maiz…Promoter2×35Sp and AtU6-26pAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
PB-PE
Plasmid#162796PurposeAll-in-one prime editor piggyBac transposonDepositorInsertSpCas9_ H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
UseCRISPR and Synthetic Biology; Piggybac transposonTagsSV40 NLSExpressionMammalianMutationPromoterCAGAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-endo-GsCT
Plasmid#205516PurposeEndosome-targeted C-terminal fragment of human Gαs protein (Gαs inhibitory peptide).DepositorInsertendo-GsCT (GNAS Human, Synthetic)
UseTags2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and mChe…ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
All-in-one lenti-BE4-NG
Plasmid#200448PurposeLentivirus vector for base editingDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-Flag-cMyc T58A
Plasmid#20075DepositorInsertcMyc (MYC Human)
UseRetroviralTagsFlagExpressionMammalianMutationT58APromoterAvailable sinceJan. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
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p39sheLL
Plasmid#40627DepositorInsertsHPV39 L1
HPV39 L2
UseTagsExpressionMammalianMutationCodon optimized for mammalian expressionPromoterCMV and Polio IRESAvailable sinceOct. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Puro-sgNUAK2
Plasmid#138693PurposeExpresses a human NUAK2-targeting sgRNA and Cas9DepositorInsertsgNUAK2 human (NUAK2 Human)
UseLentiviralTagsExpressionMutationPromoterhU6Available sinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR1a-tagRFP-KDEL
Plasmid#114177PurposeGateway entry clone containing tagRFP with a signal peptide and KDEL ER retention signal for expression of tagRFP in the ERDepositorInserttagRFP-KDEL
UseGateway entry cloneTagsKDEL retention signal and signal peptideExpressionMutationPromoterAvailable sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
EF1a_RUNX1_P2A_Hygro_Barcode
Plasmid#120475PurposeBarcoded lentiviral vector to express RUNX1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertRUNX1 (RUNX1 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDRIVE-CAG-hFX-HA
Plasmid#14994DepositorInserthuman frataxin cDNA (FXN Human)
UseTagsAU1 and HAExpressionMammalianMutationPromoterAvailable sinceAug. 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-GRAB_eCB2.0mut
Plasmid#164607PurposeExpress the endocannabinoid non-responsive sensor GRAB_eCB2.0 in Cre positive neuronsDepositorInsertEndocannabinoid non-responsive sensor GRAB_eCBmut
UseAAVTagsExpressionMutationS383T, F177APromoterhSynAvailable sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1 IntS8 AA 1-308
Plasmid#196905PurposeExpresses His-tagged Drosophila IntS8 amino acids 1-308DepositorInsertIntegrator subunit 8 (IntS8 Fly)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti4/TO-hEPO
Plasmid#50437Purposeexpress human erythropoietin under the control of tetracycline-regulatable CMV promoter using lentiviral delivery systemDepositorInsertEPO (EPO Human)
UseLentiviral; Tetracycline-regulatable (t-rex)TagsExpressionMammalianMutationPromoterCMV/TOAvailable sinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
MiniCoopR mitfa:NRAS
Plasmid#118847PurposeExpresses human NRAS Q61R mutant and zebrafish mitfa specifically in zebrafish melanocytesDepositorInsertNRAS Q61R (NRAS Human, Zebrafish)
UseZebrafish expression, tol3TagsExpressionMutationNRAS Q61RPromoterAvailable sinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only