We narrowed to 76,952 results for: Rest

1. pKP1683

   Plasmid

   #167622

   Purpose

   Express TonB C18G, V206C.

   Depositor

   Kathleen Postle

   Article

   Postle et al bioRxiv 2021.12.08.471779

   Insert

   tonB C18G, V206C
   

   Expression

   Bacterial

   Mutation

   tonB with C18G, V206C mutations

   Available Since

   July 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKP1362

   Plasmid

   #167615

   Purpose

   Express TonB C18G.

   Depositor

   Kathleen Postle

   Article

   Postle et al bioRxiv 2021.12.08.471779

   Insert

   tonB C18G
   

   Expression

   Bacterial

   Mutation

   tonB with C18G mutation

   Available Since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKP1684

   Plasmid

   #167630

   Purpose

   Express TonB C18G, R214C.

   Depositor

   Kathleen Postle

   Article

   Postle et al bioRxiv 2021.12.08.471779

   Insert

   tonB C18G, R214C
   

   Expression

   Bacterial

   Mutation

   tonB with C18G, R214C mutations

   Available Since

   June 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKP476

   Plasmid

   #167594

   Purpose

   Express TonB ∆(199-216)

   Depositor

   Kathleen Postle

   Article

   Postle et al bioRxiv 2021.12.08.471779

   Insert

   tonB ∆(199-216)
   

   Expression

   Bacterial

   Mutation

   tonB ∆(199-216)

   Available Since

   May 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKP372

   Plasmid

   #167593

   Purpose

   Express TonB frameshift of amphipathic helix

   Depositor

   Kathleen Postle

   Article

   Postle et al bioRxiv 2021.12.08.471779

   Insert

   tonB fs
   

   Expression

   Bacterial

   Mutation

   tonB with frameshift of amphipathic helix

   Available Since

   May 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pANY4-pL18-ccdB

   Plasmid

   #164898

   Purpose

   Two valuable constructs for either IPTG-induced or optimized temperature-induced expression can be simultaneously obtained in a single cloning process by utilizing plasmid pANY4-pL18-ccdB.

   Depositor

   Yingfeng An

   Insert

   ccdB cassette
   

   Tags

   His-tag

   Expression

   Bacterial

   Available Since

   April 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUPD2 nickase D10A Cas9 (GB1691)

   Plasmid

   #160588

   Purpose

   Nickase D10A Cas9 for Nt fusion.

   Depositor

   Diego Orzaez

   Insert

   nickase D10A Cas9
   

   Use

   CRISPR and Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. J-CDAN1A-5

   Plasmid

   #166522

   Purpose

   scFv of a human scaffold targeting Codanin-1. Antigen coverage aa 840-1202 of 1227

   Depositor

   Susanne Gräslund

   Insert

   single chain-Fv, scFv
   

   Use

   Recombinant antibody expression

   Tags

   3xFLAG, His6 and OmpA leader sequence

   Expression

   Bacterial

   Promoter

   LacZ

   Available Since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. Z-OPTN-10

   Plasmid

   #166562

   Purpose

   scFv of a human scaffold targeting Optineurin. Antigen coverage aa 1-510 of 577

   Depositor

   Susanne Gräslund

   Insert

   single chain-Fv, scFv
   

   Use

   Recombinant antibody expression

   Tags

   3xFLAG, His6 and OmpA leader sequence

   Expression

   Bacterial

   Promoter

   LacZ

   Available Since

   March 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA-HisFlag-rUSP19ER-CRmut

    Plasmid

    #155253

    Purpose

    for mammalian overexpression of the mutated ER isoform of rat USP19

    Depositor

    Simon Wing

    Insert

    USP19 (ER isoform) CR mutant (Usp19 Rat)
    

    Tags

    6XHis-3XFlag

    Expression

    Mammalian

    Mutation

    Cys(545) to Arg

    Promoter

    CMV

    Available Since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA-HisFlag-rUSP19ER deltaN3

    Plasmid

    #155243

    Purpose

    for mammalian overexpression of truncated (delta1-1631) ER isoform of rat USP19

    Depositor

    Simon Wing

    Insert

    USP19 (delta1-1631) (ER isoform) (Usp19 Rat)
    

    Tags

    6XHis-3XFlag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA-HisFlag-rUSP19ER deltaN2

    Plasmid

    #155242

    Purpose

    for mammalian overexpression of truncated (delta1-1088) ER isoform of rat USP19

    Depositor

    Simon Wing

    Insert

    USP19 (delta1-1088) (ER isoform) (Usp19 Rat)
    

    Tags

    6XHis-3XFlag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA-HisFlag-rUSP19ER deltaN1

    Plasmid

    #155241

    Purpose

    for mammalian overexpression of truncated (delta1-536) ER isoform of rat USP19

    Depositor

    Simon Wing

    Insert

    USP19 (delta1-536) (ER isoform) (Usp19 Rat)
    

    Tags

    6XHis-3XFlag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAS9id_TRP

    Plasmid

    #141254

    Purpose

    Galactose-inducible expression of Cas9 for addressing two targets; Contains two guide RNA expression cassettes with stuffer and Kpn restriction sites.

    Depositor

    Adam Bertl

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    GAL1 (Cas9), pSNR52 (gRNA)

    Available Since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCRII-TOPO CMV-cGFP-SV40 pA + mMALAT1_3'

    Plasmid

    #91803

    Purpose

    Expresses a cGFP mRNA that ends in the MALAT1 triple helix when the upstream SV40 poly(A) signal fails to be used

    Depositor

    Jeremy Wilusz

    Insert

    Coral Green Fluorescent Protein (cGFP)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSMPUW_8.3_M4_CAMKIIA-gateway-V5-SV40-Zeo

    Plasmid

    #128463

    Purpose

    lentiviral destination vector with CAMKIIA promoter, C-terminal V5 tag, SV40 promoter, and Zeo Resistance gene

    Depositor

    Lindy Barrett

    Insert

    CAMKIIA promoter, C-terminal V5 tag, SV40 promoter, and Zeocin Resistance gene
    

    Use

    Lentiviral

    Available Since

    Jan. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX335 Mouse 5' Srcap gRNA B

    Plasmid

    #127905

    Purpose

    Cas9 D10A Nickase Vector targeting the 5' end of the mouse Srcap gene

    Depositor

    Mark Groudine

    Insert

    Srcap gRNA
    

    Use

    CRISPR

    Available Since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX335 Mouse 5' Srcap gRNA A

    Plasmid

    #127904

    Purpose

    Cas9 D10A Nickase Vector targeting the 5' end of the mouse Srcap gene

    Depositor

    Mark Groudine

    Insert

    Srcap gRNA
    

    Use

    CRISPR

    Available Since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX330 Mouse 3' Ubf gRNA

    Plasmid

    #127902

    Purpose

    WT Cas9 Vector targeting the 3' end of the mouse Ubf gene

    Depositor

    Mark Groudine

    Insert

    gRNA for Mouse 3' Ubf
    

    Use

    CRISPR

    Available Since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pZDonor-U6-myogenin stop codon gRNA

    Plasmid

    #69549

    Purpose

    Expresses guide RNA targeting near the stop codon of myogenin in the mouse genome.

    Depositor

    Charles Gersbach

    Insert

    Myogenin C terminus-targeting guide RNA
    

    Use

    CRISPR

    Available Since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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21. CMV-Lin28a-as

    Plasmid

    #120528

    Purpose

    ssRNA expression for dsRNA production

    Depositor

    Petr Svoboda

    Insert

    Lin28 (Lin28a Mouse)
    

    Use

    RNAi

    Available Since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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22. CAG-EGFP-Lin28IR

    Plasmid

    #120517

    Purpose

    dsRNA expression

    Depositor

    Petr Svoboda

    Insert

    Lin28 (Lin28a Mouse)
    

    Use

    RNAi

    Available Since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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23. U6Lin28a-as

    Plasmid

    #120536

    Purpose

    ssRNA expression for dsRNA production

    Depositor

    Petr Svoboda

    Insert

    Lin28 (Lin28a Mouse)
    

    Use

    RNAi

    Available Since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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24. U6-Lin28a-s

    Plasmid

    #120535

    Purpose

    ssRNA expression for dsRNA production

    Depositor

    Petr Svoboda

    Insert

    Lin28 (Lin28a Mouse)
    

    Use

    RNAi

    Available Since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. CMV-Lin28a-s

    Plasmid

    #120527

    Purpose

    ssRNA expression for dsRNA production

    Depositor

    Petr Svoboda

    Insert

    Lin28 (Lin28a Mouse)
    

    Use

    RNAi

    Available Since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. CMV-Lin28aIR

    Plasmid

    #120524

    Purpose

    dsRNA expression

    Depositor

    Petr Svoboda

    Insert

    Lin28 (Lin28a Mouse)
    

    Use

    RNAi

    Available Since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pJR288

    Plasmid

    #78550

    Purpose

    For expressing small noncoding RNAs from U6 promoter. One step cloning of oiigonucleotide pairs containing CACC and AAAA overhangs. Ef1a driven mCherry visible marker.

    Depositor

    John Rossi

    Type

    Empty backbone

    Use

    RNAi

    Expression

    Mammalian

    Promoter

    U6, EF1a

    Available Since

    Jan. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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28. pmSod1-Q42L-N’His

    Plasmid

    #117708

    Purpose

    mutation on stability/solubility of SOD1

    Depositor

    Elizabeth Fisher

    Insert

    Sod1 (Sod1 Mouse)
    

    Tags

    His tag with Thrombin cleavage site

    Expression

    Bacterial

    Mutation

    Q42L

    Promoter

    T7

    Available Since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pmSod1-Q123A-N’His

    Plasmid

    #117709

    Purpose

    mutation on stability/solubility of SOD1

    Depositor

    Elizabeth Fisher

    Insert

    Sod1 (Sod1 Mouse)
    

    Tags

    His tag with Thrombin cleavage site

    Expression

    Bacterial

    Mutation

    Q123A

    Promoter

    T7

    Available Since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pmSod1-D83G-N’His

    Plasmid

    #117702

    Purpose

    mutation on stability/solubility of SOD1

    Depositor

    Elizabeth Fisher

    Insert

    Sod1 (Sod1 Mouse)
    

    Tags

    His tag with Thrombin cleavage site

    Expression

    Bacterial

    Mutation

    D83G

    Promoter

    T7

    Available Since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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