We narrowed to 18,937 results for: Tec

1. pDEST_FLAG-HUWE1_YH/GG

   Plasmid

   #187123

   Purpose

   mammalian cell expression of FLAG-HUWE1 Y355G/H356G

   Depositor

   Eric Fischer

   Article

   Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

   Insert

   HUWE1 (HUWE1 Human)
   

   Tags

   1xFLAG

   Expression

   Mammalian

   Mutation

   Y355G/H356G

   Available Since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCellFree_G03 GTF2E1

   Plasmid

   #67089

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   GTF2E1 (GTF2E1 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

3. 1352wUV2 F1bbs kan GCGTGGNNN

   Plasmid

   #18753

   Depositor

   Scot Wolfe

   Article

   Meng et al Nat Biotechnol. 2008 Jun . 26(6):695-701

   Insert

   3 tandem zinc fingers
   

   Expression

   Bacterial

   Mutation

   A Kan cassette (1090bp) is inserted between two b…

   Available Since

   July 11, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCellFree_G03 EBNA1BP2

   Plasmid

   #67072

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   EBNA1BP2 EBNA1BP2 EBNA1 binding protein 2 (EBNA1BP2 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pCfB3036(XI-1 MarkerFree)

   Plasmid

   #73274

   Purpose

   EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-1 (Chr XI: 67491..68573)

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Expression

   Yeast

   Available Since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-LmoCas8b2

   Plasmid

   #126488

   Purpose

   Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B Flag-Cas8b2 driven by hUbC promoter

   Depositor

   Charles Gersbach

   Article

   Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

   Insert

   LmoCas8b2
   

   Use

   CRISPR and Lentiviral

   Tags

   Flag, NLS

   Expression

   Mammalian

   Promoter

   hUbC

   Available Since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-LmoCas7

   Plasmid

   #126489

   Purpose

   Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B Flag-Cas7 driven by hUbC promoter

   Depositor

   Charles Gersbach

   Article

   Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

   Insert

   LmoCas7
   

   Use

   CRISPR and Lentiviral

   Tags

   Flag, NLS

   Expression

   Mammalian

   Promoter

   hUbC

   Available Since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-LmoCas5

   Plasmid

   #126490

   Purpose

   Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B Flag-Cas5 driven by hUbC promoter

   Depositor

   Charles Gersbach

   Article

   Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

   Insert

   LmoCas5
   

   Use

   CRISPR and Lentiviral

   Tags

   Flag, NLS

   Expression

   Mammalian

   Promoter

   hUbC

   Available Since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-LmoCas6

   Plasmid

   #126491

   Purpose

   Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B Flag-Cas6 driven by hUbC promoter

   Depositor

   Charles Gersbach

   Article

   Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

   Insert

   LmoCas6
   

   Use

   CRISPR and Lentiviral

   Tags

   Flag, NLS

   Expression

   Mammalian

   Promoter

   hUbC

   Available Since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV asyn S87E

    Plasmid

    #36057

    Depositor

    Hilal Lashuel

    Article

    Oueslati et al J Neurosci. 2012 Feb 1;32(5):1536-44.

    Insert

    alpha-synuclein S87E (SNCA Human)
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    S87E

    Promoter

    CMV

    Available Since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

11. [pSK475] pLJC5 HA-Depdc5(A)

    Plasmid

    #109344

    Purpose

    Lenti-viral expression of HA-tagged Depdc5 mutant A

    Depositor

    David Sabatini, Kuang Shen

    Article

    Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

    Insert

    Depdc5 (DEPDC5 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Mutation

    DIYGD(185-189)GSGSG

    Available Since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. [pSK476] pLJC5 HA-Depdc5(B)

    Plasmid

    #109345

    Purpose

    Lenti-viral expression of HA-tagged Depdc5 mutant B

    Depositor

    David Sabatini, Kuang Shen

    Article

    Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

    Insert

    Depdc5 (DEPDC5 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Mutation

    EQPLH(371-375)GSGSG

    Available Since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. [pSK478] pLJC5 HA-Depdc5(P)

    Plasmid

    #109347

    Purpose

    Lenti-viral expression of HA-tagged Depdc5 mutant P

    Depositor

    David Sabatini, Kuang Shen

    Article

    Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

    Insert

    Depdc5 (DEPDC5 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Mutation

    YDLLP(775-779)GSGSG

    Available Since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYPQ269D

    Plasmid

    #164717

    Purpose

    Gateway entry clone (attL1 & attR5) PmCDA1-CBE_V04 for C-T base editing

    Depositor

    Yiping Qi

    Article

    Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

    Insert

    zCas9(D10A)-PmCDA1-UGI-T2A-MS2-UGI)
    

    Use

    CRISPR; Gateway compatible pmcda1-cbe_v04 entry c…

    Expression

    Plant

    Available Since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRB113.2

    Plasmid

    #181948

    Purpose

    aTc-inducible expression of NarL with C-terminal mNeonGreen fusion. Also contains mCherry under NarL-controlled promoter PdcuS

    Depositor

    Jeffrey Tabor

    Article

    Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11

    Insert

    NarL-mNG; mCherry; tetR (AX04_RS23485, narL narL - E. coli, Synthetic)
    

    Tags

    mNeonGreen

    Expression

    Bacterial

    Promoter

    NarL-mNG-PLtetO-1; mCherry-PdcuS; tetR-J23106

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pspCas9-ABE-3'UTR-sgRNA-2xMS2

    Plasmid

    #132554

    Purpose

    Vector plasmid expressing ABEmax and sgRNA scaffold with MS2 replacing the Tetraloop and loop 2

    Depositor

    Baisong Lu

    Article

    Lyu et al CRISPR J. 2021 Feb;4(1):69-81. doi: 10.1

    Inserts

    ABEmax
    sgRNA, with Tetraloop and loop 2 replaced by MS2 aptamer
    

    Expression

    Mammalian

    Available Since

    Feb. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAM4896 (pCV0008)

    Plasmid

    #120081

    Purpose

    Broad host range plasmid. Bacteria plasmid vector to test various parts and devices. Expression of GFP and a nourseothricin resistance gene in various cyanobacteria.

    Depositor

    Susan Golden

    Article

    Taton et al Nucleic Acids Res. 2014 Jul 29. pii: gku

    Insert

    Plasmid assembled from 3 CYANO-VECTOR modular devices: CV-RSF1010Y25F, CV-nat1_A7120, CV-PconII-LTRBS-GFPmut2
    

    Use

    Other

    Mutation

    mobA Y25F

    Available Since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. Nanog-CreER targeting construct

    Plasmid

    #59720

    Purpose

    Cre-ER knockin targeting construct for mouse endogenous Nanog locus

    Depositor

    Jacob Hanna

    Article

    Maza et al Nat Biotechnol. 2015 Jun 22. doi: 10.103

    Insert

    creERT2
    

    Use

    Mouse Targeting

    Promoter

    mNanog

    Available Since

    Jan. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDARMO_CMVT_3xFLAG-HUWE1_H3962D

    Plasmid

    #187131

    Purpose

    mammalian cell expression of 3xFLAG-HUWE1 H3962D

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

    Insert

    HUWE1 (HUWE1 Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    H3962D

    Available Since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDsRed-HP1alpha

    Plasmid

    #179702

    Purpose

    visualization of centromere heterochromatin

    Depositor

    Kenji Sugimoto

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2014;78(4):55

    Insert

    heterochromatin protein alpha (CBX5 Human)
    

    Tags

    dsRed

    Expression

    Mammalian

    Available Since

    March 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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