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phage UbiC NLS HA stdMCP Halo

Plasmid

#104098

Purpose

Lentiviral vector expressing synonymous tandem MS2 coat protein fused to Halo. Used to label reporter mRNAs.

Depositor

Jeffrey Chao

Article

Horvathova et al Mol Cell. 2017 Nov 2;68(3):615-625.e9. d

Insert

stdMCP-Halo

Use

Lentiviral

Tags

C-terminal Halo-tag, N-terminal NLS, and Two syno…

Expression

Mammalian

Available Since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR1a-tagRFP-KDEL

Plasmid

#114177

Purpose

Gateway entry clone containing tagRFP with a signal peptide and KDEL ER retention signal for expression of tagRFP in the ER

Depositor

Nicolas Demaurex

Article

Nunes-Hasler et al Nat Commun. 2017 Nov 24;8(1):1852. doi:

Insert

tagRFP-KDEL

Use

Gateway entry clone

Tags

KDEL retention signal and signal peptide

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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HA-MAP1LC3A

Plasmid

#137756

Purpose

Expression of LC3A

Depositor

Carol Mercer

Article

Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

Insert

MAP1LC3A (MAP1LC3A Human)

Tags

HA and myc

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pHIV-IL13-PDGFRStalk-TM

Plasmid

#240243

Purpose

Lentiviral transfer plasmid encoding IL-13 with PDGFR stalk and TM domain

Depositor

Michael Birnbaum

Article

Dobson et al Nat Methods. 2022 Apr;19(4):449-460. doi

Insert

IL-13 (IL13 Human)

Use

Lentiviral

Tags

EGFP and HA

Expression

Mammalian

Promoter

EF1a

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA-mSin1(1.1)-HA

Plasmid

#73388

Purpose

Expresses mSin-HA

Depositor

Jie Chen, Taekjip Ha

Article

Jain et al Proc Natl Acad Sci U S A. 2014 Dec 16;11

Insert

mSin1 transcript variant protein isoform 1 (MAPKAP1 Human)

Tags

HA tag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR_P2R-P3_R2-Turbo-NES-mVenus-STOP-L3

Plasmid

#127353

Purpose

gateway entry vector for making C-terminal TurboID-mVenus fusion with non-nuclear proteins

Depositor

Dominique Bergmann

Article

Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

Insert

TurboID (BirA mutant)

Use

Gateway entry vector

Tags

GS linker, NES, V5, and mVenus

Mutation

Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

Promoter

no promoter

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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Flag-betaPIXa

Plasmid

#15234

Depositor

Rick Horwitz

Article

Mayhew et al J Proteome Res. 2006 Sep . 5(9):2417-23.

Insert

beta-PIXa (Arhgef7 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Sept. 13, 2007

Availability

Academic Institutions and Nonprofits only

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Flag-ECFP-betaPIXa

Plasmid

#15235

Depositor

Rick Horwitz

Article

Mayhew et al J Proteome Res. 2006 Sep . 5(9):2417-23.

Insert

beta-PIXa (Arhgef7 Mouse)

Tags

ECFP and Flag

Expression

Mammalian

Available Since

Sept. 13, 2007

Availability

Academic Institutions and Nonprofits only
VRK1_HUMAN_D0

Plasmid

#79684

Purpose

This plasmid encodes the kinase domain of VRK1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

VRK1 (VRK1 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pEGFPC1-ORAI1-EC-HA

Plasmid

#174335

Purpose

encodes hORAI1 with HA-tag in its extracellular loop 2 and an N-terminal GFP fusion (GFP-ORAI1-EC-HA).

Depositor

Sonal Srikanth

Article

Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

Insert

human Orai1 (ORAI1 Human)

Tags

GFP in the N-terminus and HA tag in the extracell…

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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