We narrowed to 14,439 results for: NTS

1. pGEX-6P-2_dCrk

   Plasmid

   #131146

   Purpose

   Expresses GST::dCrk (based on crk isoform RA).

   Depositor

   Mark Peifer

   Article

   Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

   Insert

   Crk (Crk Fly)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOPO685

   Plasmid

   #195300

   Purpose

   pBAD322-MycaIDCas-PaqCI, arapBAD promoter expressing type I-D Cascade and one spacer array of McCAST (Tn7575), the single spacer is replaced with PaqCI entry site.

   Depositor

   Joseph Peters

   Article

   Hsieh et al Nucleic Acids Res. 2023 Jan 25;51(2):765

   Insert

   McCAST Cascade and native array with PaqCI entry site
   

   Expression

   Bacterial

   Promoter

   Arabinose inducible arapBAD

   Available Since

   May 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. ZIKV_Trigger_32B

   Plasmid

   #75009

   Purpose

   Portion of Zika Virus genome (KU312312: 7166-7299) containing sensor 32B trigger sequence

   Depositor

   Alexander Green, James Collins

   Article

   Pardee et al Cell. 2016 May 6. pii: S0092-8674(16)305

   Insert

   ZIKV_Trigger_32B
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. SPLINTR BFP v1 backbone

   Plasmid

   #179779

   Purpose

   SPLINTR backbone vector

   Depositor

   Mark Dawson

   Article

   Fennell et al Nature. 2022 Jan;601(7891):125-131. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBSFI-myr-akt-T308A

   Plasmid

   #49189

   Purpose

   cDNA of Akt1 with myristyolation signal and point mutation from T to A at position 308 in cloning vector

   Depositor

   Peter Vogt

   Article

   Aoki et al Proc Natl Acad Sci U S A. 1998 Dec 8;95(

   Insert

   Akt1 (Akt1 Mouse)
   

   Use

   Adaptor plasmid

   Tags

   HA tag and myristolation signal

   Mutation

   T308A

   Promoter

   T7

   Available Since

   Dec. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pJG367

   Plasmid

   #91185

   Purpose

   BeYDV replicon T-DNA for gene targeting in tobacco leaves, H840A double nickase (nAtCas9_H840A+gNt_R2+gNt_F2+donor)

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   nAtCas9_H840A+gNt_R2+gNt_F2+donor
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJG382

   Plasmid

   #91189

   Purpose

   BeYDV replicon T-DNA for gene targeting in tobacco leaves, D10A double nickase (nAtCas9_D10A+gNt_R2+gNt_F2+donor)

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   nAtCas9_D10A+gNt_R2+gNt_F2+donor
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. VQR variant

   Plasmid

   #138560

   Purpose

   Expresses human codon-optimized SpCas9-VQR and blasticidin resistance: EFS promoter-VQR-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

   Insert

   VQR
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   D1135V, R1335Q, T1337R

   Promoter

   EFS

   Available Since

   June 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGP-CMV-jGCaMP8f F366H

   Plasmid

   #204141

   Purpose

   Fluorescent reporter for calcium imaging in mammalian cells - jGCaMP8f with F366H mutation

   Depositor

   Katalin Torok

   Article

   Tran et al Front Cell Neurosci. 2023 May 18;17:1155

   Insert

   jGCaMP8f F366H
   

   Tags

   N-terminal His tag

   Expression

   Mammalian

   Mutation

   Phenylalanine 366 changed to histidine

   Promoter

   CMV

   Available Since

   Dec. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. LentiU6-hMYOD1 gRNA_multi1-3-MS2-Puro

    Plasmid

    #192683

    Purpose

    Lentiviral expression of multi gNAs targeting hMYOD1 promoter to activate human MYOD1 transcription

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    Human MYOD1 activating gRNAs #1,2,3 (MYOD1 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. tdp43-EGFP construct11

    Plasmid

    #28204

    Depositor

    Zuoshang Xu

    Article

    Yang et al PLoS One. 2010 . 5(12):e15878.

    Insert

    Tar DNA binding protein (TARDBP Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    aa193-274 only

    Available Since

    Sept. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

12. pC1300_pUB10_pco-vCASphi_E9t_V2_CmYLCVp_35sT_ribozyme_AtPDS3_gRNA10

    Plasmid

    #197966

    Purpose

    T-DNA binary vector to express pco-vCasphi driven by UBQ10 gene promoter, and the AtPDS3 guide RNA 10 driven by CmYLCVp, flanked by ribozymes.

    Depositor

    Steven Jacobsen

    Article

    Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

    Inserts

    pco-vCasphi-2
    AtPDS3 gRNA10
    

    Use

    CRISPR

    Expression

    Plant

    Promoter

    CmYLCVp and pUBQ10

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRK5-MYC-ITFG2

    Plasmid

    #87046

    Purpose

    mammalian expression of ITFG2

    Depositor

    David Sabatini

    Article

    Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

    Insert

    ITFG2 (ITFG2 Human)
    

    Tags

    MYC

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. DCT.VV.Orf37

    Plasmid

    #12725

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jack Bennink, Jon Yewdell

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf37
    

    Expression

    Mammalian

    Available Since

    Dec. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

15. DCT.VV.Orf48

    Plasmid

    #12736

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jack Bennink, Jon Yewdell

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf48
    

    Expression

    Mammalian

    Available Since

    Dec. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

16. pAT9751-BEAR-mCherry-preedited

    Plasmid

    #162993

    Purpose

    BEAR control plasmid with split mCherry and intact 5' splice site

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    mCherry split with an intron between amino acids 119-120
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-CAG-eYFP-3x-miR708-5p-TS

    Plasmid

    #117381

    Purpose

    An AAV genome containing miRNA target sequence (TS) 708-5p to reduce expression of the fluorescent protein eYFP in neurons

    Depositor

    Viviana Gradinaru

    Article

    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

    Insert

    EYFP + 3 copies of miR708: CCCAGCTAGATTGTAAGCTCCTT
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDIRECT_21B

    Plasmid

    #91129

    Purpose

    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9_dead + AtU6:gRNA, Plant Selection: 2x35S:hpt II

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Engineering Reagent: 35S:AtCas9_dead + AtU6:gRNA
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    D10A, H840A

    Available Since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFAB3689

    Plasmid

    #47834

    Purpose

    BIOFAB RFP reporter plasmid for measuring promoter 3 + BCD1 efficiency.

    Depositor

    Drew Endy

    Article

    Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

    Insert

    promoter 3 and BCD1
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    see insert

    Available Since

    Dec. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEX-4T2 SHP2 E76A

    Plasmid

    #8324

    Depositor

    Ben Neel

    Article

    O'Reilly et al Mol Cell Biol 2000 Jan;20(1):299-311.

    Insert

    SHP2 (PTPN11 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    E76A (activated mutant)

    Available Since

    June 17, 2005

    Availability

    Academic Institutions and Nonprofits only