We narrowed to 59,364 results for: cloning

1. PB-CT3G-CERP2-MG-IRES2-mTagBFP2

   Plasmid

   #175509

   Purpose

   All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mTagBFP2 (CMVe-hEF1a-driven rtTA and puromycin resistance)

   Depositor

   George Church

   Article

   Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   TRE3G-mCMV

   Available Since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pQStrep2-PREI3

   Plasmid

   #31592

   Depositor

   Konrad Buessow

   Article

   Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

   Insert

   preimplantation protein 3 (MOB4 Human)
   

   Tags

   His and StrepII-tag

   Expression

   Bacterial

   Available Since

   April 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pENT-L1-Kozak-lacZ-stop-L2

   Plasmid

   #186359

   Purpose

   Entry clone with ORF encoding lacZ flanked by Gateway recombination sequences.

   Depositor

   Patrick Lemaire

   Article

   Roure et al PLoS One. 2007. 2(9):e916.

   Insert

   lacZ (lacZ E. coli, Synthetic)
   

   Use

   Expression of lacz reporter

   Available Since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. Donor_eGP2AP_RC

   Plasmid

   #133784

   Purpose

   Library reporter vector/integration vector. Contains library cloning sites MluI and SpeI upstream of a terminator and downstream of a BxB1 attB site. Library integration occurs through BxB1 site.

   Depositor

   Sriram Kosuri

   Article

   Davis et al Cell Syst. 2020 Jul 22;11(1):75-85.e7. d

   Type

   Empty backbone

   Tags

   eGFP-P2A-Puro

   Expression

   Mammalian

   Available Since

   June 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. CMV Flag PAI-RBP

   Plasmid

   #45452

   Depositor

   Thomas Gelehrter

   Article

   Heaton et al J Biol Chem. 2001 Feb 2;276(5):3341-7. E

   Insert

   PAI-RBP (SERBP1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   June 20, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pNP1 sfGFP toehold switch

   Plasmid

   #107355

   Purpose

   Expresses sfGFP (a GFP) in Ecoli off a T7 promoter when pNP1 sfGFP trigger is co-expressed

   Depositor

   James Collins

   Article

   Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

   Insert

   toehold 7 + sfGFP
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDD315 (mTurquoise2^SEC^2xHA)

   Plasmid

   #73343

   Purpose

   mTurquoise2^SEC^2xHA vector with ccdB sites for cloning homology arms

   Depositor

   Bob Goldstein

   Article

   Zhang et al J Cell Biol. 2023 Sep 4;222(9):e20230210

   Insert

   mTurquoise2-C1^SEC^3xFlag
   

   Use

   CRISPR and Cre/Lox

   Tags

   2xHA and C. elegans codon-optimized mTurquoise2

   Expression

   Worm

   Available Since

   March 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSLC-242

   Plasmid

   #73189

   Purpose

   Template plasmid which encodes chloramphenicol resistance gene for positive selection and a toxin gene (relE) under the control of rhamnose induceable promoter (PrhaB) for negative selection.

   Depositor

   Swaine Chen

   Article

   Khetrapal et al Nucleic Acids Res. 2015 Jul 27;43(13):e8

   Insert

   chloramphenicol (cat Although Ampicillin gene is present, it was inactivated while cloning.)
   

   Expression

   Bacterial

   Promoter

   chloramphenicol promoter

   Available Since

   July 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC19/mChe-C

   Plasmid

   #183160

   Purpose

   Plant transient expression vector containing mCherry tag. Unique nucleotide sequences Uc and Ud for cloning at C terminus of mCherry.

   Depositor

   Yao-Guang Liu, Qinlong Zhu

   Article

   Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

   Type

   Empty backbone

   Expression

   Plant

   Available Since

   April 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYL322d1/N-eGFP

    Plasmid

    #183151

    Purpose

    Plant transient expression vector containing eGFP tag. Unique nucleotide sequences Uc and Ud for cloning at N terminus of eGFP.

    Depositor

    Yao-Guang Liu, Qinlong Zhu

    Article

    Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUC19/eCFP-C

    Plasmid

    #183156

    Purpose

    Plant transient expression vector containing eCFP tag. Unique nucleotide sequences Uc and Ud for cloning at C terminus of eCFP

    Depositor

    Yao-Guang Liu, Qinlong Zhu

    Article

    Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. p5E-smyhc1

    Plasmid

    #126748

    Purpose

    Zebrafish slow myosin heavy chain promoter. Clone 667

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    smyhc1 (smyhc1 Zebrafish)
    

    Use

    Multisite gateway entry clone

    Available Since

    June 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA5/FRT/TO DNAJB1

    Plasmid

    #19468

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    DNAJB1 (DNAJB1 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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14. pQTEV-STMN1

    Plasmid

    #31326

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    stathmin 1/oncoprotein 18 (STMN1 Human)
    

    Tags

    His and TEV

    Expression

    Bacterial

    Available Since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. PresentER-MSIIFFLPL (GFP)

    Plasmid

    #102946

    Purpose

    PresentER minigene encoding H2-Kb ligand MSIIFFLPL (GFP)

    Depositor

    David Scheinberg

    Article

    Gejman et al Elife. 2018 Nov 30;7. pii: 41090. doi: 1

    Insert

    MSIIFFLPL (Serpinf1 Mouse, Synthetic)
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pQTEV-PTD004

    Plasmid

    #34798

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    GTP-binding protein PTD004 (OLA1 Human)
    

    Tags

    His and TEV

    Expression

    Bacterial

    Available Since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCIFR-3

    Plasmid

    #233295

    Purpose

    Integration of msfGFP (or your Module Of Interest) in a random fashion via Tn5 insertion. The antibiotic cassette used for selecting for the integration can be removed in a later stage with pFNC.

    Depositor

    Pablo Ivan Nikel

    Article

    Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1

    Insert

    msfGFP
    

    Promoter

    p14G

    Available Since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR221_SLC6A3

    Plasmid

    #132160

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Sijben et al Sci Rep. 2021 Jan 14;11(1):1312. doi: 10

    Insert

    SLC6A3 (SLC6A3 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 22, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pDT-sgRNA-XMAS-1x

    Plasmid

    #164413

    Purpose

    Dual targeted guide and cloning vector. Targets pEF-XMAS-1xStop. Guides targeting endogenous sites can be cloned into BbsI sites.

    Depositor

    Xiao Wang

    Article

    Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10

    Insert

    sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. bdSUMO-E5TAT-GFP-HiBit

    Plasmid

    #170993

    Purpose

    For bacterial expression of bdSUMO tagged muGFP with E5TAT peptide and NanoLuc complementation peptide variant #86

    Depositor

    Angus Johnston

    Article

    Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

    Insert

    14x-His-bdSUMO-E5TAT-muGFP-HiBiT
    

    Use

    Luciferase

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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