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Plasmid#196538PurposeExpression of GST-Eps8L2_367-496DepositorInsertEps8L2_367-496 (EPS8L2 Human)
UseTagsGSTExpressionBacterialMutationPromoterT7 promoter; lac UV5 promoterAvailable sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST15-Eps8L2_494-546
Plasmid#196539PurposeExpression of GST-Eps8L2_494-546DepositorInsertEps8L2_494-546 (EPS8L2 Human)
UseTagsGSTExpressionBacterialMutationPromoterT7 promoter; lac UV5 promoterAvailable sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti4/V5-DEST-LASSIE
Plasmid#192105PurposeLentiviral vector expressing long non-coding RNA LASSIE (linc00520)DepositorInsertLASSIE
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.2/V5-DEST-Noa1
Plasmid#191969PurposeOverexpression of Nitric Oxide Associated 1 protein (NOA1), tagged with V5.DepositorInsertNoa1
UseSynthetic BiologyTagsV5ExpressionMutationPromoterAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe3-RfA-HA
Plasmid#186374PurposeDestination vector for the expression of a fusion of an N-ter protein of interest with an HA tag into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe3-HA-RfA
Plasmid#186373PurposeDestination vector for the expression of a fusion of N-ter HA tag and a protein of interest into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe3-RfA
Plasmid#186370PurposeDestination vector for the expression of a protein of interest into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST_FLAG-HUWE1_delta169-189
Plasmid#187136Purposemammalian cell expression of FLAG-HUWE1 delta169-189DepositorInsertHUWE1 (HUWE1 Human)
UseTags1xFLAGExpressionMammalianMutationdelta169-189PromoterAvailable sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28_redesigned_Caspase-4_ANR261ENP_D315H_M318K_T352K_enzymatic domain
Plasmid#183387PurposeOverexpression of catalytic domain of inflammatory caspase in bacteriaDepositorInsertredesigned Caspase-4 (AA94-377) (CASP4 Human)
UseTagsHis-TEVExpressionBacterialMutationANR261ENP, D315H, M318K, T352KPromoterT7Available sinceJuly 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pdestMB14_grh-1p-grh-1-gfp
Plasmid#177761PurposeOverexpression of C. elegans grh-1DepositorInsertgrh-1 (grh-1 Nematode)
UseTagsExpressionWormMutationPromoterAvailable sinceApril 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-DEST42-dre4
Plasmid#170440PurposeExpress drosophila dre4 in E.coli expression system. Generated from pDONR-dre4 by Gateway system. Used for custom antibody purification.DepositorInsertdre4 (dre4 Fly)
UseTags6xHis and V5ExpressionBacterialMutationDeleted amino acids 1-20PromoterT7Available sinceJuly 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-DEST42 -SSRP1
Plasmid#170439PurposeExpress drosophila SSRP1 in E.coli expression system. Generated from pDONR-SSRP1 by Gateway system. Used for custom antibody purification.DepositorInsertssrp1 (Ssrp Fly)
UseTags6xHis and V5ExpressionBacterialMutationPromoterT7Available sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-cDAXX-0SB
Plasmid#169730PurposeBacterial expression of N-terminally 6His tagged cDAXX-0SBDepositorInsertcDAXX-0SB (DAXX Human)
UseTags6 HisExpressionBacterialMutationComprises residues 495-741 and has the major SB m…PromoterT7Available sinceJune 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDEST 527-PHL2
Plasmid#118670PurposeBacterial overexpressionDepositorInsertPHL2 (AT3G24120 Mustard Weed)
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDEST 527-XERICO
Plasmid#118671PurposeBacterial overexpressionDepositorInsertXERICO (XERICO Mustard Weed)
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDEST 544-PHL2
Plasmid#118672PurposeBacterial overexpressionDepositorInsertPHL2 (AT3G24120 Mustard Weed)
UseTagsHis6-NusAExpressionBacterialMutationPromoterT7Available sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDEST 544-XERICO
Plasmid#118673PurposeBacterial overexpressionDepositorInsertXERICO (XERICO Mustard Weed)
UseTagsHis6-NusAExpressionBacterialMutationPromoterT7Available sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDEST 565-PHL2
Plasmid#118674PurposeBacterial overexpressionDepositorInsertPHL2 (AT3G24120 Mustard Weed)
UseTagsHis6-GSTExpressionBacterialMutationPromoterT7Available sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDEST 565-XERICO
Plasmid#118675PurposeBacterial overexpressionDepositorInsertXERICO (XERICO Mustard Weed)
UseTagsHis6-GSTExpressionBacterialMutationPromoterT7Available sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDEST 566-PHL2
Plasmid#118676PurposeBacterial overexpressionDepositorInsertPHL2 (AT3G24120 Mustard Weed)
UseTagsHis6-MBPExpressionBacterialMutationPromoterT7Available sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDEST 566-XERICO
Plasmid#118677PurposeBacterial overexpressionDepositorInsertXERICO (XERICO Mustard Weed)
UseTagsHis6-MBPExpressionBacterialMutationPromoterT7Available sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Rab26 in pCSDEST2
Plasmid#140884PurposeExpression of zebrafish GFP-tagged Rab26 in mammalian cells.DepositorInsertRab26 (rab26 Zebrafish)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Rab40b in pCSDEST2
Plasmid#140883PurposeExpression of zebrafish GFP-tagged Rab40b in mammalian cells.DepositorInsertRab40b (rab40b Zebrafish)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Rab13 in pCSDEST2
Plasmid#140879PurposeExpression of zebrafish GFP-tagged Rab13 in mammalian cells.DepositorInsertRab13 (rab13 Zebrafish)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-CaaX(tH) in pCSDEST2
Plasmid#140890PurposeExpression of GFP-CaaX (sequence from Hras) in mammalian cells.DepositorInsertCaaX membrane targeting motif (H-ras)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC-DEST-R1R4
Plasmid#141054PurposeMutisite Gateway mediated vector constructionDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC-DEST-R5R4
Plasmid#141055PurposeMutisite Gateway mediated vector constructionDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-2XFL-Src-S4-UD
Plasmid#140305PurposeExpresses Flag-tagged human Src-S4-UD in mammalian cells for structure-function studiesDepositorInsertSrc deletion mutant (SRC Human)
UseTags2X FlagExpressionMammalianMutationcontains only the SH4 and UD regionsPromoterCMVAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-2XFL-Src-S4-UD-KD
Plasmid#140301PurposeExpresses Flag-tagged human Src-S4-UD-KD in mammalian cells for structure-function studiesDepositorInsertSrc deletion mutant (SRC Human)
UseTags2X FlagExpressionMammalianMutationlacks the SH3 and SH2 domainsPromoterCMVAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
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JK104: pMVP-DEST
Plasmid#121851PurposepMVP destination vector, empty expression plasmid backboneDepositorTypeEmpty backboneUsePmvp gateway destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEF-DEST51 PNRC1
Plasmid#123293PurposeExpression of PNRC1 in mammalian cellsDepositorInsertPNRC1 (PNRC1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
CMYA5tnC/pDEST26-N-FLAG
Plasmid#98130PurposeExpress CMYA5 (aa 3859 – 4069) with a N-terminal FLAG tag in pDEST26 mammalian expression vectorDepositorInsertCMYA5 (CMYA5 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceAug. 21, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
CMYA5tnT/pDEST26-N-FLAG
Plasmid#98131PurposeExpress CMYA5 (aa 3859 – 4069) Leu allele (4063) with a N-terminal FLAG tag in pDEST26 mammalian expression vectorDepositorInsertCMYA5 (CMYA5 Human)
UseTagsFLAGExpressionMammalianMutationrs10043986 (NM_153610.4:c.12188C>T)PromoterCMVAvailable sinceAug. 3, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEJS427-pCSDest2-AcrE2
Plasmid#85677PurposeMammalian expression of anti-CRISPR protein from Type I-EDepositorInsertType I-E anti-CRISPR
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-ARL4-V5-His6
Plasmid#67440PurposeMammalian expression of hArl4 with C-term V5 and His6 tagDepositorInsertARL4 (ARL4A Human)
UseTagsHis and V5ExpressionMammalianMutationbp 105 A to T; silent mutationPromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-ARL5A-V5-His6
Plasmid#67441PurposeMammalian expression of hArl5 with C-term V5 and His6 tagDepositorInsertARL5A (ARL5A Human)
UseTagsHis and V5ExpressionMammalianMutationbp 70 C to T; silent mutationPromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-ARL5B-V5-His6
Plasmid#67442PurposeMammalian expression of hArl5b with C-term V5 and His6 tagDepositorInsertARL5B (ARL5B Human)
UseTagsHis and V5ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-ARL4C-V5-His6
Plasmid#67444PurposeMammalian expression of hArl4c with C-term V5 and His6 tagDepositorInsertARL7 (ARL4C Human)
UseTagsHis and V5ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-ARL4D-V5-His6
Plasmid#67447PurposeMammalian expression of hArl4D with C-term V5 and His6 tagDepositorInsertARL4D (ARL4D Human)
UseTagsHis and V5ExpressionMammalianMutationbp 272 A to C; N91T mutation; bp 327 T to G; sil…PromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only