We narrowed to 5,623 results for: crispr cas9 grna plasmid

1. pS23.U6<ActB.mus_C-term-KI_gRNA

   Plasmid

   #207408

   Purpose

   gRNA from a U6 promoter targeting the mouse ActB near the 3' end of the ORF. Used for C-terminal knockin by DSB repair. [Lab plasmid ID: TU260]

   Depositor

   Alexandros Poulopoulos

   Article

   Richardson et al CRISPR J. 2023 Oct;6(5):447-461. doi: 10

   Insert

   ActB
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Nov. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. 1183_pAAV-U6-Ex14-gRNAd-CB-EmGFP

   Plasmid

   #89061

   Purpose

   AAV-gRNA targeting the murine Ldlr gene

   Depositor

   William Lagor

   Article

   Jarrett et al Sci Rep. 2017 Mar 16;7:44624. doi: 10.10

   Insert

   EmGFP
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken beta actin with partial CMV enhancer

   Available since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. 1184_pAAV-U6-ApoB-gRNA2-CB-EmGFP

   Plasmid

   #89062

   Purpose

   AAV-gRNA targeting the murine Apob gene

   Depositor

   William Lagor

   Article

   Jarrett et al Sci Rep. 2017 Mar 16;7:44624. doi: 10.10

   Insert

   EmGFP
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken beta actin with partial CMV enhancer

   Available since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX330-U6-E690_guide-CBh-hSpCas9

   Plasmid

   #188545

   Purpose

   Plasmid encoding pCas9 and gRNA for mutagenesis or gene-correction of human ABCA3 mutation at amino acid E690

   Depositor

   Darrell Kotton

   Article

   Sun et al J Clin Invest. 2024 Jan 16;134(2):e16427

   Insert

   gRNA
   

   Use

   CRISPR

   Tags

   T2A-EGFP

   Expression

   Mutation

   Promoter

   U6

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX330-U6-W308_guide-CBh-hSpCas9

   Plasmid

   #188546

   Purpose

   Plasmid encoding pCas9 and gRNA for mutagenesis or gene-correction of human ABCA3 mutation at amino acid W308

   Depositor

   Darrell Kotton

   Article

   Sun et al J Clin Invest. 2024 Jan 16;134(2):e16427

   Insert

   gRNA
   

   Use

   CRISPR

   Tags

   T2A-EGFP

   Expression

   Mutation

   Promoter

   U6

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX330-U6-L101_guide-CBh-hSpCas9

   Plasmid

   #188547

   Purpose

   Plasmid encoding pCas9 and gRNA for mutagenesis or gene-correction of human ABCA3 mutation at amino acid L101

   Depositor

   Darrell Kotton

   Article

   Sun et al J Clin Invest. 2024 Jan 16;134(2):e16427

   Insert

   gRNA
   

   Use

   CRISPR

   Tags

   T2A-EGFP

   Expression

   Mutation

   Promoter

   U6

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAS9-mCherry empty

   Plasmid

   #80975

   Purpose

   Cas9, mCherry, and sgRNA expression

   Depositor

   Veit Hornung

   Article

   Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAS9-BFP empty

   Plasmid

   #80976

   Purpose

   Cas9, TagBFP, and sgRNA expression

   Depositor

   Veit Hornung

   Article

   Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJEP10-AAV-U6/TO-gRNA(Empty)-CMV-TetR-P2A-eGFP-KASH-pA

   Plasmid

   #82706

   Purpose

   AAV backbone with a full length U6/TO promoter driving expression of a sgRNA. The gRNA can be inserted via Sapl sites. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.

   Depositor

   Jonathan Ploski

   Article

   de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do

   Insert

   U6/TO Empty gRNA Expression Cassette
   

   Use

   AAV and CRISPR

   Tags

   CMV promoter driving expression of TetR-P2A-GFP-K…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJEP12-AAV-H1/TO-L-sgRNA(Empty)-CMV-TetR-P2A-eGFP-KASH-pA

    Plasmid

    #82704

    Purpose

    AAV backbone with a full length H1/TO promoter driving expression of a sgRNA. The gRNA can be inserted via Sapl sites. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.

    Depositor

    Jonathan Ploski

    Article

    de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do

    Insert

    H1/TO-L Empty gRNA Expression Cassette
    

    Use

    AAV and CRISPR

    Tags

    CMV promoter driving expression of TetR-P2A-GFP-K…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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