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pXPR_003 sgINTS12 guide 2

Plasmid

#193607

Purpose

INTS12 knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgINTS12 guide 2 (INTS12 Human)

Use

Lentiviral

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_003 sgINTS12 guide 1

Plasmid

#193606

Purpose

INTS12 knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgINTS12 guide 1 (INTS12 Human)

Use

Lentiviral

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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TTR C10S E63C

Plasmid

#190009

Purpose

Expression of human TTR in E coli

Depositor

Peter Wright

Article

Sun et al J Biol Chem. 2022 Jun 18;298(8):102162.

Insert

TTR (TTR Human)

Expression

Bacterial

Mutation

C10S, E63C

Promoter

T7

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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BII-ChBtW-CTNNB1_S33Y

Plasmid

#133376

Purpose

Piggybac vector for constitutive expression

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

CTNNB1 (CTNNB1 Human)

Tags

Flag

Expression

Mammalian

Mutation

S33Y

Promoter

CMV enhancer and hEf1a (CpG free)

Available Since

Jan. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pTone-gata2aECE-nsGFP

Plasmid

#132975

Purpose

gata2a endothelial enhancer (x6) and basal promoter driving nuclear-localized sfGFP in pTol1 backbone

Depositor

Nathan Lawson

Article

Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

Inserts

nuclear localized sfGFP
gata2a endothelial enhancer (x6) with a carp b-actin basal promoter

Use

Unspecified; Transposon-mediated

Tags

6x myc and SV40 NLS

Available Since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pEMS2235

Plasmid

#111888

Purpose

High copy number plasmid containing Ple320 (POU4F1 MiniPromoter) insert.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

Ple320 (POU4F1 Human)

Use

Unspecified

Promoter

Ple320 (POU4F1 MiniPromoter)

Available Since

Aug. 10, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS2236

Plasmid

#111889

Purpose

High copy number plasmid containing Ple321 (TUBB3 MiniPromoter) insert.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

Ple321 (TUBB3 Human)

Use

Unspecified

Promoter

Ple321 (TUBB3 MiniPromoter)

Available Since

Aug. 10, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS2025

Plasmid

#49132

Purpose

AAV plasmid with NOV (Ple303) promoter driving expression of iCre.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-Ple303-iCre

Use

AAV

Expression

Mammalian

Promoter

NOV

Available Since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pEGFP C1 SADBs shRNA Resistant

Plasmid

#67155

Purpose

pEGFP containing N-terminally EGFP Tagged mouse SADBs resistant to an shRNA

Depositor

Maria Alvarado-Kristensson, Ana Carrera

Article

Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

Insert

Brsk1 (Brsk1 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

nucleotide 294 C-T, nucleotide 297 C-G

Promoter

CMV

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only
pTER SADB shRNA Human

Plasmid

#67151

Purpose

Doxycycline-regulated mammalian expression vector for expressing shRNA against human SADB

Depositor

Maria Alvarado-Kristensson, Ana Carrera

Article

Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

Insert

BRSK1 (BRSK1 Human)

Use

RNAi

Expression

Mammalian

Promoter

H1 Tet

Available Since

July 7, 2015

Availability

Academic Institutions and Nonprofits only
PA-mCherry-miniSOG-H2B-C-10

Plasmid

#57789

Purpose

Localization: Nucleus/Histones, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

H2B (H2BC11 Human)

Tags

PA-mCherry-miniSOG

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available Since

Jan. 29, 2015

Availability

Academic Institutions and Nonprofits only
mKO-Zyxin-6

Plasmid

#57853

Purpose

Localization: Focal Adhesions, Excitation: 548, Emission: 561

Depositor

Michael Davidson

Article

Shroff et al Proc Natl Acad Sci U S A. 2007 Dec 18;10

Insert

Zyxin (ZYX Human)

Tags

mKO

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
mTFP1-FYN-N-10

Plasmid

#55483

Purpose

Localization: Membrane Associated Kinase, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

FYN (FYN Human)

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only
SL542

Plasmid

#49951

Purpose

Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding site

Depositor

Keith Joung

Article

Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

Insert

Tet1CD RH-8 (RHOXF2 Human)

Use

TALEN

Promoter

EF1a

Available Since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only
SL544

Plasmid

#49970

Purpose

Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding site

Depositor

Keith Joung

Article

Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

Insert

Tet1CD RH-10 (RHOXF2 Human)

Use

TALEN

Promoter

EF1a

Available Since

Dec. 10, 2013

Availability

Academic Institutions and Nonprofits only
SL545

Plasmid

#49953

Purpose

Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding site

Depositor

Keith Joung

Article

Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

Insert

Tet1CD RH-11 (RHOXF2 Human)

Use

TALEN

Promoter

EF1a

Available Since

Dec. 10, 2013

Availability

Academic Institutions and Nonprofits only
SL543

Plasmid

#49952

Purpose

Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding site

Depositor

Keith Joung

Article

Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

Insert

Tet1CD RH-9 (RHOXF2 Human)

Use

TALEN

Promoter

EF1a

Available Since

Dec. 10, 2013

Availability

Academic Institutions and Nonprofits only
SL541

Plasmid

#49950

Purpose

Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding site

Depositor

Keith Joung

Article

Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

Insert

Tet1CD RH-7 (RHOXF2 Human)

Use

TALEN

Promoter

EF1a

Available Since

Dec. 10, 2013

Availability

Academic Institutions and Nonprofits only
SL540

Plasmid

#49949

Purpose

Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding site

Depositor

Keith Joung

Article

Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

Insert

Tet1CD RH-6 (RHOXF2 Human)

Use

TALEN

Promoter

EF1a

Available Since

Dec. 10, 2013

Availability

Academic Institutions and Nonprofits only
pIS58 TIP120A 3'UTR mut

Plasmid

#14502

Depositor

David Bartel

Article

Lim et al Nature. 2005 Feb 17. 433(7027):769-73.

Insert

TIP120A 3'UTR mutant (CAND1 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

CATTCC’s were changed to AAGTAC's

Available Since

March 7, 2007

Availability

Academic Institutions and Nonprofits only