We narrowed to 59,366 results for: cloning

1. pLC133

   Plasmid

   #198639

   Purpose

   Expression plasmid for dCas9 in E. coli used in Chip-seq experiments. dCas9 is tagged with a C-terminal 3x FLAG tag. A guide RNA can be cloned using BsaI restriction sites.

   Depositor

   David Bikard

   Article

   Rostain et al Nucleic Acids Res. 2023 Mar 17:gkad170.

   Insert

   dCas9
   

   Tags

   3x-FLAG

   Expression

   Bacterial

   Promoter

   pTet

   Available Since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pACYC-T7-Nla-repeat-BbsI

   Plasmid

   #180215

   Purpose

   For cloning Neisseria lactamica Type I-C crRNA for protein purification in E.Coli

   Depositor

   Yan Zhang

   Article

   Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. epi-ABEmax

   Plasmid

   #135974

   Purpose

   Expresses ABEmax, EBNA1 and blasticidin resistence gene; cloning backbone for sgRNA; Contains Epstein-Barr virus oriP replication origin

   Depositor

   Yongming Wang

   Article

   Qi et al Front Cell Dev Biol. 2020 Sep 25;8:59058

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   May 5, 2020

   Availability

   Academic Institutions and Nonprofits only

4. pCPP5060

   Plasmid

   #128716

   Purpose

   Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor clone

   Depositor

   Alan Collmer

   Article

   Lopez-Solanilla et al Mol Microbiol. 2004 Oct;54(2):353-65. do

   Insert

   shcN-hopN1
   

   Expression

   Bacterial

   Available Since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCPP6450

   Plasmid

   #128731

   Purpose

   Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

   Depositor

   Alan Collmer

   Article

   Wei et al Cell Host Microbe. 2015 Jun 10;17(6):752

   Insert

   HopAD1
   

   Expression

   Bacterial

   Available Since

   Jan. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pQTEV-SCP2

   Plasmid

   #31336

   Depositor

   Konrad Buessow

   Article

   Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

   Insert

   sterol carrier protein 2 (SCP2 Human)
   

   Tags

   His and TEV

   Expression

   Bacterial

   Available Since

   Nov. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFB-LIC-Bse

   Plasmid

   #26108

   Purpose

   SGC Baculovirus transfer vector with His tag in 22-aa N-terminal fusion peptide with TEV protease cleavage site. Includes sites for LIC cloning, and SacB gene for negative selection

   Depositor

   Opher Gileadi

   Article

   Savitsky et al J Struct Biol. 2010 Oct;172(1):3-13. doi

   Type

   Empty backbone

   Use

   Baculovirus expression

   Tags

   His6 - TEV

   Expression

   Insect

   Promoter

   polyhedrin

   Available Since

   Oct. 4, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCK015

   Plasmid

   #105347

   Purpose

   Bacterial translocation fo proteins into plant cells

   Depositor

   Johannes Stuttmann

   Article

   Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

   Insert

   secretion signal, ccdB cassette
   

   Use

   Bacterial secretion

   Mutation

   BsaI restriction sites removed

   Available Since

   Aug. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCRII-1.1-2.01 beta

   Plasmid

   #69591

   Purpose

   OT-II TCR beta chain cDNA in pCRII

   Depositor

   Francis R. Carbone

   Article

   Barnden et al Immunol Cell Biol. 1998 Feb;76(1):34-40.

   Insert

   TCR beta clone 1.1 (Tcrb Mouse)
   

   Expression

   Bacterial

   Available Since

   Oct. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPE3-pPuro

    Plasmid

    #215722

    Purpose

    Cloning vector for Uni-Vector prime editing system

    Depositor

    Kou-Juey Wu

    Article

    Huang et al Biotechnol J. 2024 Feb;19(2):e2300353. d

    Insert

    PE2
    

    Use

    Unspecified

    Promoter

    EFS promoter

    Available Since

    Dec. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCK013

    Plasmid

    #105345

    Purpose

    Bacterial translocation fo proteins into plant cells

    Depositor

    Johannes Stuttmann

    Article

    Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

    Insert

    secretion signal, ccdB cassette
    

    Use

    Bacterial secretion

    Mutation

    BsaI restriction sites removed

    Available Since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCK016

    Plasmid

    #105348

    Purpose

    Bacterial translocation for proteins into plant cells

    Depositor

    Johannes Stuttmann

    Article

    Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

    Insert

    secretion signal, ccdB cassette
    

    Use

    Bacterial secretion

    Mutation

    BsaI restriction sites removed

    Available Since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCK014

    Plasmid

    #105346

    Purpose

    Bacterial translocation fo proteins into plant cells

    Depositor

    Johannes Stuttmann

    Article

    Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

    Insert

    secretion signal, ccdB cassette
    

    Use

    Bacterial secretion

    Mutation

    BsaI restriction sites removed

    Available Since

    May 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. BB1_12_pGAP

    Plasmid

    #98498

    Purpose

    BB1 with insert: promoter PGAP

    Depositor

    Brigitte Gasser, Diethard Mattanovich, Michael Sauer

    Article

    Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

    Insert

    GAP promoter
    

    Expression

    Bacterial

    Mutation

    Internal BsaI and BpiI sites removed from insert

    Available Since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pME-nCas9n-P2A-TagBFP2-pA

    Plasmid

    #176026

    Purpose

    Multisite gateway vector for expression of nCas9n with blue reporter. Parton lab clone ENT

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    nCas9n-P2A-TagBFP2
    

    Use

    Gateway entry clone

    Available Since

    Oct. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCFJ1209

    Plasmid

    #44491

    Depositor

    Erik Jorgensen

    Article

    Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

    Insert

    Minimal Mos1 [Peft-3 tdTomato(NLS) tbb-2 UTR, NeoR]
    

    Use

    Gateway expression clone

    Available Since

    April 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBSc + MShal

    Plasmid

    #16166

    Depositor

    Larry Salkoff

    Article

    Pak et al Proc Natl Acad Sci U S A. 1991 May 15. 8

    Insert

    Mus musculus potassium voltage-gated channel (Kcnd1 Mouse)
    

    Use

    Xenopus oocyte expression

    Mutation

    N/A

    Available Since

    Jan. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

18. CaV2.1 (pCR3.1)

    Plasmid

    #26573

    Depositor

    Diane Lipscombe

    Article

    Richards et al Eur J Neurosci. 2007 Nov . 26(10):2950-6

    Insert

    Cacna1a (Cacna1a Mouse)
    

    Expression

    Mammalian

    Available Since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

19. pCRISPRai

    Plasmid

    #189938

    Purpose

    Expresses CRISPRai cassette under EF1a promoter

    Depositor

    Yu-Chen Hu

    Article

    Truong et al Nucleic Acids Res. 2019 Jul 26;47(13):e7

    Insert

    CRISPRai cassette
    

    Expression

    Mammalian

    Promoter

    CMV enhancer-rat EF1 alpha

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA5/FRT/TO V5 DNAJA4

    Plasmid

    #19521

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    DnaJ (Hsp40) homolog, subfamily A, member 4 (DNAJA4 Human)
    

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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