We narrowed to 5,623 results for: crispr cas9 grna plasmid

1. pX330-BsaIx2-DD-Cas9

   Plasmid

   #75380

   Purpose

   Expresses DD-SpCas9 in mammalian cells and has a cloning site for an sgRNA. The FKBP12 L106P destabilization domain allows for inducible stabilization of Cas9 through the addition of Shield-1

   Depositor

   Michele Calos

   Article

   Geisinger et al Nucleic Acids Res. 2016 Jan 13. pii: gkv

   Insert

   DD-SpCas9
   

   Use

   CRISPR

   Tags

   FKBP12 L106P DD

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pICH86966::AtU6p::sgRNA:NbCysP6

   Plasmid

   #223217

   Purpose

   Expresses an sgRNA targeting the NbCysP6 gene in Nicotiana benthamiana with an Arabidopsis U6 promoter

   Depositor

   Tsepo Tsekoa

   Article

   Singh et al Front Plant Sci. 2022 Aug 18;13:953654.

   Insert

   NbCysP6 sgRNA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   None

   Expression

   Plant

   Mutation

   Promoter

   Arabidopsis U6 promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pdCas9-Tet1-CD

   Plasmid

   #83340

   Purpose

   To introduce dCas9-fused Tet1-CD and sgRNA2.0 system

   Depositor

   Ronggui Hu

   Article

   Xu et al Cell Discov. 2016 May 3;2:16009. doi: 10

   Insert

   dCas9-Tet1-CD and sgRNA scaffold with 2xMS2 binding sites
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   U6, CBH

   Available since

   Oct. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSpCas9(BB)-2A-Puro-SMCR8

   Plasmid

   #83440

   Purpose

   Expresses Cas9 and a gRNA targeting SMCR8

   Depositor

   Shawn Ferguson

   Article

   Amick et al Mol Biol Cell. 2016 Aug 24. pii: mbc.E16

   Insert

   gRNA targeting human SMCR8 (SMCR8 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. lenti sgRNA(MS2)_puro optimized backbone

   Plasmid

   #73797

   Purpose

   optimized lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

   Depositor

   Feng Zhang

   Article

   Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and EF1A

   Available since

   March 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEJS1096 Dual-sgRNA.Design 1

   Plasmid

   #159538

   Purpose

   Delivery of dual sgRNA cassettes and Nme2Cas9 in a single AAV vector

   Depositor

   Erik Sontheimer

   Article

   Ibraheim et al Nat Commun. 2021 Nov 1;12(1):6267. doi:

   Insert

   Nme2Cas9 nuclease with two guide RNA cassettes with promoters
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   U1a

   Available since

   Dec. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CMV-Dpi2Cas9-puro

   Plasmid

   #232113

   Purpose

   Expresses Dpi2Cas9 and puromycin resistance genes, and cloning backbone for sgRNA

   Depositor

   Yongming Wang

   Type

   Empty backbone

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-CMV-Slu1Cas9-puro

   Plasmid

   #232111

   Purpose

   Expresses Slu1Cas9 and puromycin resistance genes, and cloning backbone for sgRNA

   Depositor

   Yongming Wang

   Type

   Empty backbone

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-CMV-Slu2Cas9-puro

   Plasmid

   #232112

   Purpose

   Expresses Slu2Cas9 and puromycin resistance genes, and cloning backbone for sgRNA

   Depositor

   Yongming Wang

   Type

   Empty backbone

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-CMV-SeqCas9-puro

    Plasmid

    #232110

    Purpose

    Expresses SeqCas9 and puromycin resistance genes, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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