We narrowed to 59,366 results for: cloning

1. pYL322d1/eGFP-C

   Plasmid

   #183152

   Purpose

   Plant transient expression vector containing eGFP tag. Unique nucleotide sequences Uc and Ud for cloning at C terminus of eGFP.

   Depositor

   Yao-Guang Liu, Qinlong Zhu

   Article

   Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

   Type

   Empty backbone

   Expression

   Plant

   Available Since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5/FRT/TO HSPA2

   Plasmid

   #19458

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

   Insert

   HSPA2 (HSPA2 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 6, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. HBT_pcoCASphi_version2

   Plasmid

   #197932

   Purpose

   Cloning vector with Arabidopsis codon optimized Casphi-2 protein, with SV40 NLS at the C-terminal

   Depositor

   Steven Jacobsen

   Article

   Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

   Insert

   pcoCasphi-2
   

   Use

   CRISPR

   Tags

   2xNLS-2xFLAG

   Expression

   Plant

   Promoter

   35SPPDK promoter

   Available Since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCPP5108

   Plasmid

   #128726

   Purpose

   Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

   Depositor

   Alan Collmer

   Article

   Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

   Insert

   hopAA1-2
   

   Expression

   Bacterial

   Available Since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCPP5107

   Plasmid

   #128725

   Purpose

   Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

   Depositor

   Alan Collmer

   Article

   Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

   Insert

   hopAA1-1
   

   Expression

   Bacterial

   Available Since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYPQ-NF-L3-NF-L1-dMb2Cas12a

   Plasmid

   #177686

   Purpose

   Gateway entry clone (attL1 & attR5) for a single chain FokI-dMb2Cas12a genome editing

   Depositor

   Yiping Qi

   Article

   Cheng et al Biotechnol J. 2022 Apr 4:e2100571. doi:

   Insert

   2xFokI-dMb2Cas12a
   

   Use

   CRISPR; Gateway compatible 2xfoki-dmb2cas12a entr…

   Tags

   NLS

   Expression

   Plant

   Available Since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMA_Int

   Plasmid

   #110096

   Purpose

   E. coli replicative vector which contains the mycophage integrase gene required for the attP/attB integration. Is co-transformed in trans together with pFLAG_attP, but cannot replicate in mycobacteria, and is thus lost shortly after electroporation.

   Depositor

   Markus Seeger

   Article

   Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

   Insert

   mycophage integrase
   

   Use

   Required for transformation of attb integrating v…

   Available Since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA5/FRT/TO GFP HSPA2

   Plasmid

   #19485

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

   Insert

   heat shock 70kDa protein 2 (HSPA2 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Oct. 6, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA5/FRT/TO V5 DNAJB8

   Plasmid

   #19531

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

   Insert

   DnaJ (Hsp40) homolog, subfamily B, member 8 (DNAJB8 Human)
   

   Tags

   V5

   Expression

   Mammalian

   Available Since

   Oct. 6, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. pQTEV-YWHAE

    Plasmid

    #31562

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon (YWHAE Human)
    

    Tags

    His and TEV

    Expression

    Bacterial

    Available Since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pENTR-AtTAS1c-B/c

    Plasmid

    #51774

    Purpose

    Gateway-compatible entry vector for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor

    Depositor

    James Carrington

    Article

    Carbonell et al Plant Physiol. 2014 Mar 19.

    Insert

    AtTAS1c-B/c
    

    Use

    Gateway-compatible entry clone

    Mutation

    A. thaliana TAS1c precursor sequence including a …

    Available Since

    April 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pQTEV-PSMD10

    Plasmid

    #31332

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    PSMD10, proteasome (prosome, macropain) 26S subunit, non-ATPase, 10 (gankyrin) (PSMD10 Human)
    

    Tags

    His and TEV

    Expression

    Bacterial

    Available Since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR221 CARD8

    Plasmid

    #169962

    Purpose

    pDONR221 for Gateway shuttling. Contains the CARD8 T60 isoform (no stop codon)

    Depositor

    Daniel Bachovchin

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Gateway cloning vector

    Available Since

    July 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. PB-CT3G-ERP2-MG-IRES2-mNeonGreen

    Plasmid

    #175506

    Purpose

    All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mNeonGreen (hEF1a-driven rtTA and puromycin resistance)

    Depositor

    George Church

    Article

    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    TRE3G-mCMV

    Available Since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA5/FRT/TO GFP DNAJB4

    Plasmid

    #19499

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    DNAJB4 (DNAJB4 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. BB3rN_14*

    Plasmid

    #98550

    Purpose

    empty BB3 for insertion and overexpression of 1 gene in P. pastoris

    Depositor

    Brigitte Gasser, Diethard Mattanovich, Michael Sauer

    Article

    Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

    Type

    Empty backbone

    Expression

    Bacterial and Yeast

    Available Since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA5/FRT/TO V5 DNAJB7

    Plasmid

    #19530

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    DnaJ (Hsp40) homolog, subfamily B, member 7 (DNAJB7 Human)
    

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLV U6-sgRNA UbC-GFP

    Plasmid

    #106948

    Purpose

    sgRNA cloning cassette using BsmBI/Esp3I enzymes with GFP marker

    Depositor

    Albert Edge

    Article

    Lenz et al Front Cell Dev Biol. 2019 Feb 19;7:14. d

    Insert

    sgRNA BsmBI/Esp3I cloning site
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pYPQ166-iSpyMac

    Plasmid

    #149553

    Purpose

    Gateway entry clone for CRISPR-iSpyMacCas9 genome editing at NAAR PAMs

    Depositor

    Yiping Qi

    Article

    Sretenovic et al Plant Commun. 2020 Jul 22;2(2):100101. d

    Insert

    iSpyMacCas9
    

    Use

    CRISPR; Gateway compatible zispymaccas9 entry clo…

    Tags

    3X FLAG, NLS and NLS

    Expression

    Plant

    Mutation

    R221K, N394K, Protospacer interacting domain form…

    Available Since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA5/FRT/TO HSPH3

    Plasmid

    #19455

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    heat shock 70kDa protein 4-like (HSPA4L Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 7, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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