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pSS2 - pBait-1xMS2-7GC[empty]-TtrpA

Plasmid

#222406

Purpose

arabinose-induced synthesis of an RNA containing an MS2 hairpin, 7GC clamp and trpA terminator. Used as a three-hybrid control. For cloning purposes, see plasmid #222404.

Depositor

Katherine Berry

Article

Nguyen et al Sci Rep. 2025 Jan 30;15(1):3820. doi: 10

Type

Empty backbone

Expression

Bacterial

Promoter

pBAD

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pOSV00157

Plasmid

#222937

Purpose

Kill switch plasmid for DNA sensor construction using Bacillus subtilis

Depositor

Ophelia Venturelli

Article

Cheng et al Nat Commun. 2023 Apr 10;14(1):2001. doi:

Insert

TxpA-RatA toxin-antitoxin

Expression

Bacterial

Promoter

Phyperspank

Available Since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pBbA2C_Ptet::lasI

Plasmid

#189576

Purpose

Las synthase (3OC12-HSL)

Depositor

Daniel Ducat

Article

Kokarakis et al ACS Synth Biol. 2023 Jan 20;12(1):265-27

Insert

Acyl-homoserine-lactone synthase

Use

Synthetic Biology

Expression

Bacterial

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pLei-sfGFP-D134*

Plasmid

#191110

Purpose

An E. coli sfGFP reporter with one TAG at position 134

Depositor

Han Xiao

Article

Wu et al Protein Sci. 2022 Oct;31(10):e4443. doi:

Insert

superfolder green fluorescence protein

Tags

His tag

Expression

Bacterial

Mutation

changing D134 to TAG

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pLei-sfGFP-V2*D134*

Plasmid

#191111

Purpose

An E. coli sfGFP reporter with two TAG at positions 2 & 134

Depositor

Han Xiao

Article

Wu et al Protein Sci. 2022 Oct;31(10):e4443. doi:

Insert

superfolder green fluorescence protein

Tags

His tag

Expression

Bacterial

Mutation

changing V2 & D134 to TAG

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pSLC25A13-APEX2

Plasmid

#228198

Purpose

plasmid encoding human SLC25A13 with an APEX protein tag

Depositor

Stephen Benkovic

Article

Sha et al J Biol Chem. 2024 Aug 2;300(9):107620. d

Insert

SLC25A13 (SLC25A13 Human)

Use

Synthetic Biology

Tags

APEX2

Promoter

M13

Available Since

Nov. 20, 2024

Availability

Academic Institutions and Nonprofits only

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gRNA_CloningVector_tagBFP

Plasmid

#163747

Purpose

Empty gRNA cloning vector, contains a tagBFP fluorescent marker for FACS sorting after transfection.

Depositor

Jennifer Mitchell

Article

Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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gRNA_CloningVector_miRFP670

Plasmid

#163748

Purpose

Empty gRNA cloning vector, contains a miRFP670 fluorescent marker for FACS sorting after transfection.

Depositor

Jennifer Mitchell

Article

Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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T. aq sigmaA

Plasmid

#215350

Purpose

Express T. aq sigmaA transcription initiation factor in E. coli cells

Depositor

Julius Lucks

Article

Hertz et al Nucleic Acids Res. 2024 May 8;52(8):4466

Insert

rpoD

Tags

6x His

Expression

Bacterial

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pET3a-Xl-H3∆Nterm[R42]

Plasmid

#221201

Purpose

Xenopus laevis histone H3 with N-terminal tail deleted.

Depositor

Gregory Bowman

Article

Nodelman et al Nat Struct Mol Biol. 2022 Feb;29(2):121-

Insert

Xl-H3∆Nterm[R42]

Expression

Bacterial

Available Since

July 11, 2024

Availability

Academic Institutions and Nonprofits only

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