We narrowed to 5,623 results for: crispr cas9 grna plasmid

1. pX552-hSyn-DIO-GCaMP8f_Grin1TTT gRNA

   Plasmid

   #215282

   Purpose

   Expresses Grin1 Control gRNA in a Cre dependent GCaMP8f vector

   Depositor

   Bryan Copits

   Article

   Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

   Insert

   Grin1 control gRNA
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pICH86966::AtU6p::sgRNA:NbVPE1a/b

   Plasmid

   #223218

   Purpose

   Expresses an sgRNA targeting the NbVPE1a/b genes in Nicotiana benthamiana with an Arabidopsis U6 promoter

   Depositor

   Tsepo Tsekoa

   Article

   Singh et al Front Plant Sci. 2022 Aug 18;13:953654.

   Insert

   NbVPE1a/b
   

   Use

   CRISPR and Synthetic Biology

   Tags

   None

   Expression

   Plant

   Mutation

   Promoter

   Arabidopsis U6 promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSpCas9(BB)-2A-Puro (PX459) V2.0

   Plasmid

   #62988

   Purpose

   Cas9 from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Has Service

   Cloning Grade DNA

   Insert

   hSpCas9-2A-Puro V2.0
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   March 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSpCas9n(BB)-2A-Puro (PX462) V2.0

   Plasmid

   #62987

   Purpose

   Cas9n (D10A nickase mutant) from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Insert

   hSpCas9n-2A-Puro
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Mutation

   Cas9 D10A nickase mutant

   Promoter

   Available since

   March 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAC154-dual-dCas9VP160-sgExpression

   Plasmid

   #48240

   Purpose

   Dual expression construct expressing both dCas9VP160 and sgRNA from separate promoters

   Depositor

   Rudolf Jaenisch

   Article

   Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   HA-Tag, HA-tag, and VP160

   Expression

   Mammalian

   Mutation

   D10A H840A

   Promoter

   Available since

   Sept. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAC152-dual-dCas9VP64-sgExpression

   Plasmid

   #48238

   Purpose

   Dual expression construct expressing both dCas9VP64 and sgRNA from separate promoters

   Depositor

   Rudolf Jaenisch

   Article

   Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   HA Tag, HA-Tag, and VP64

   Expression

   Mammalian

   Mutation

   D10A;H840A

   Promoter

   Available since

   Sept. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAC153-dual-dCas9VP96-sgExpression

   Plasmid

   #48239

   Purpose

   Dual expression construct expressing both dCas9VP96 and sgRNA from separate promoters

   Depositor

   Rudolf Jaenisch

   Article

   Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   HA-Tag, HA-tag, and VP96

   Expression

   Mammalian

   Mutation

   D10A H840A

   Promoter

   Available since

   Sept. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. mCBFKOgRNA5

   Plasmid

   #84366

   Purpose

   Cas9-gRNA plasmid for mouse Cbfa2t2 Knockout

   Depositor

   Danny Reinberg

   Article

   Tu et al Nature. 2016 Jun 8;534(7607):387-90. doi

   Insert

   Cbfa2t2-gRNA5
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

9. mCBFKOgRNA2

   Plasmid

   #84365

   Purpose

   Cas9-gRNA plasmid for mouse Cbfa2t2 Knockout

   Depositor

   Danny Reinberg

   Article

   Tu et al Nature. 2016 Jun 8;534(7607):387-90. doi

   Insert

   Cbfa2t2-gRNA2
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

10. hIRF-1 gRNA #409 (Sa)

    Plasmid

    #98134

    Purpose

    Expresses a guide RNA (gRNA) to target human IRF-1 promoter for the Sa-Cas9 system

    Depositor

    Hodaka Fujii

    Article

    Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do

    Insert

    gRNA_hIRF1 promoter #409
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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