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GEARBOCS-Vamp2-Tag-HA

Plasmid

#218188

Purpose

To tag endogenous mouse VAMP2 with HA

Depositor

Cagla Eroglu

Article

Bindu et al eLife. 2024, Reviewed Preprint. 13:RP103

Insert

sgRNA (Vamp2 Mouse)

Use

AAV and CRISPR

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGEARBOCS-v0

Plasmid

#196495

Purpose

CRISPR tool to target astrocytes

Depositor

Cagla Eroglu

Article

Bindu et al eLife. 2024, Reviewed Preprint. 13:RP103

Type

Empty backbone

Use

AAV and CRISPR

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pgfaABC1D-mCherry-CAAX

Plasmid

#196488

Purpose

To over express mCherry-CAAX under gfaABC1D promoter in astrocytes

Depositor

Cagla Eroglu

Article

Bindu et al eLife. 2024, Reviewed Preprint. 13:RP103

Insert

mCherry-CAAX

Use

AAV

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGEARBOCS-v0-Gfap-KO

Plasmid

#196489

Purpose

To KO Gfap in mouse astrocytes

Depositor

Cagla Eroglu

Article

Bindu et al eLife. 2024, Reviewed Preprint. 13:RP103

Insert

gRNA

Use

AAV and CRISPR

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGEARBOCS-v0- Gfap-TagIN-mCherry

Plasmid

#196490

Purpose

To tag endogenous mouse GFAP with mCherry

Depositor

Cagla Eroglu

Article

Bindu et al eLife. 2024, Reviewed Preprint. 13:RP103

Inserts

mCherry
gRNA

Use

AAV and CRISPR

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGEARBOCS-v0-Gfap-GeneTRAP

Plasmid

#196492

Purpose

To KO and genetrap mouse GFAP simultaneously

Depositor

Cagla Eroglu

Article

Bindu et al eLife. 2024, Reviewed Preprint. 13:RP103

Inserts

mCherry-CAAX
gRNA

Use

AAV and CRISPR

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGEARBOCS-v0-Vamp2-GeneTRAP

Plasmid

#196493

Purpose

To KO and genetrap mouse VAMP2 simultaneously

Depositor

Cagla Eroglu

Article

Bindu et al eLife. 2024, Reviewed Preprint. 13:RP103

Inserts

mCherry-CAAX
gRNA

Use

AAV and CRISPR

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGEARBOCS-v0-Vamp2-TagIN-HA

Plasmid

#196494

Purpose

To tag endogenous mouse VAMP2 with HA

Depositor

Cagla Eroglu

Article

Bindu et al eLife. 2024, Reviewed Preprint. 13:RP103

Inserts

HA
gRNA

Use

AAV and CRISPR

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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PX552-mScn8a-3xHA-Syn-smFP-flag

Plasmid

#182563

Purpose

For mouse Nav1.6 knockin with 3xHA at the C-terminal

Depositor

Zhe J. Liu

Article

Liu et al J Neurosci. 2022 Jun 6;42(28):5482-98. d

Inserts

mouse Scn8a gRNA and 3xHA donor DNA
smFP-flag

Use

AAV and CRISPR

Promoter

EF1 and U6

Available Since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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PX552-mScn8a-HaloTag-V5-EF1-sfGFP

Plasmid

#182565

Purpose

For mouse Nav1.6 knockin with HaloTag-V5 at the C-terminal

Depositor

Zhe J. Liu

Article

Liu et al J Neurosci. 2022 Jun 6;42(28):5482-98. d

Inserts

mouse Scn8a gRNA and HaloTag-V5 donor DNA
sfGFP

Use

AAV and CRISPR

Promoter

EF1 and U6

Available Since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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PX552-mScn8a-3xV5-EF1-smFP-flag

Plasmid

#182562

Purpose

For mouse Nav1.6 knockin with 3xV5 at the C-terminal

Depositor

Zhe J. Liu

Article

Liu et al J Neurosci. 2022 Jun 6;42(28):5482-98. d

Inserts

mouse Scn8a gRNA and 3xV5 donor DNA
smFP-flag

Use

AAV and CRISPR

Promoter

EF1 and U6

Available Since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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Rap-MESA-20-Tev

Plasmid

#84555

Purpose

MESA protease chain with rapamycin FKBP ectodomain, 20 extracellular linkers, and Tev protease

Depositor

Joshua Leonard

Article

Hartfield et al ACS Synth Biol. 2017 Nov 17;6(11):2042-2

Insert

Rap-MESA-FKBP-20-Tev

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pVH003

Plasmid

#80397

Purpose

Contains pBAD-CusR (response regulator) and pCusR-antiscaffold-YFP-AAV. This plasmid was used for microscopy tracking experiments

Depositor

Richard Murray

Article

Hsiao et al ACS Synth Biol. 2015 Feb 20;4(2):150-61.

Inserts

Anti-scaffold - Venus YFP
CusR (cusR )
AraC (araC )

Use

Synthetic Biology

Tags

Fused by GS linker to LZx domain and Venus has N-…

Expression

Bacterial

Available Since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

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GfaABC1D-Lck-mCherry-ibARK(D110A)

Plasmid

#241242

Purpose

Negative control viral expression vecotor for plasma membrane-tethered ibARK in astrocytes

Depositor

Jun Nagai

Article

Dewa et al Nature. 2025 Oct 15. doi: 10.1038/s41586

Insert

Lck-mCherry-ibARK(D110A)

Use

AAV

Mutation

D110A

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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sgCRY2

Plasmid

#197457

Purpose

Expresses sgRNA(CRY2 gene) in mammalian cells

Depositor

Han Kyoung Choe

Article

Kim et al Sci Rep. 2021 Jan 28;11(1):2575. doi: 10

Insert

hU6-sgCRY2-1-hU6-sgCRY2-2 (Cry2 Mouse)

Use

AAV

Tags

mCherry (K14E, N210D, E249G)

Promoter

U6, hSyn

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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Sa-SchCas9

Plasmid

#192137

Purpose

Expresses Sa-SchCas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Wang et al Adv Sci (Weinh). 2022 Feb;9(4):e2104789.

Insert

Sa-SchCas9

Use

AAV

Expression

Mammalian

Available Since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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pyc128m

Plasmid

#158778

Purpose

soluble GCaMP expression

Depositor

Jennifer Garrison, Zachary Knight

Article

Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

Insert

GCaMP6m

Use

AAV

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pSLED.NPL

Plasmid

#193014

Purpose

Neuron-specific reporter (CBh promoter, Pls3 exon, bichromatic reporter)

Depositor

Jonathan Ling

Article

Ling et al Nat Commun. 2022 Oct 1;13(1):5773. doi:

Insert

Bichromatic reporter (EGFP and dsRed-Express2)

Use

AAV

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pZac2.1-GfaABC1D -IgK-NCAN ELS-HA

Plasmid

#219811

Purpose

Secreted HA-tagged NCAN-ELS domain

Depositor

Cagla Eroglu

Article

Irala et al Neuron. 2024 Mar 27:S0896-6273(24)00161-

Insert

IgK (Igk Mouse)

Use

AAV

Expression

Mammalian

Available Since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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PZac2.1 hSyn1-eGFP-SAPAP3

Plasmid

#176853

Purpose

Expresses SAPAP3 protein fused with eGFP at the N-terminus in neurons

Depositor

Baljit Khakh

Article

Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

Insert

eGFP-SAPAP3

Use

AAV

Tags

GFP

Expression

Bacterial

Promoter

hSyn1

Available Since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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