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pLPT2

Plasmid

#209974

Purpose

Contains Level 0 Part: Terminator (TpepN) for the construction of Level 1 plasmids

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

Terminator (T_pepN)

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLPR1

Plasmid

#209979

Purpose

Contains Level 0 Part: resistance cassette (ermBL) for the construction of Level 1 plasmids

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

resistance cassette (ermBL)

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLPP1

Plasmid

#209963

Purpose

Contains Level 0 Part: mock / negative control Promoter (P-nc) for the construction of Level 1 plasmids

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

Promoter (mock)

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLPP2

Plasmid

#209964

Purpose

Contains Level 0 Part: constitutive Promoter (P48) for the construction of Level 1 plasmids

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

Promoter (P48)

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLPC31

Plasmid

#209996

Purpose

Contains Level 0 Part: 3' Connector (3C2SN) for the construction of Level 1 plasmids

Depositor

Georg Fritz, Thorsten Mascher

Article

Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

Insert

3' Connector (3C2SN)

Expression

Bacterial

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pLPC32

Plasmid

#209997

Purpose

Contains Level 0 Part: 3' Connector (3C5CSN) for the construction of Level 1 plasmids

Depositor

Georg Fritz, Thorsten Mascher

Article

Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

Insert

3' Connector (3C5CSN)

Expression

Bacterial

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pLPC52

Plasmid

#209991

Purpose

Contains Level 0 Part: 5' Connector (5C2SN) for the construction of Level 1 plasmids

Depositor

Georg Fritz, Thorsten Mascher

Article

Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

Insert

5' Connector (5C2SN)

Expression

Bacterial

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pLPC51

Plasmid

#209990

Purpose

Contains Level 0 Part: 5' Connector (5C1CSN) for the construction of Level 1 plasmids

Depositor

Georg Fritz, Thorsten Mascher

Article

Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

Insert

5' Connector (5C1CSN)

Expression

Bacterial

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pRU292

Plasmid

#167687

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

PcUBQ4-2::Cas9i

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-HA-GlpG

Plasmid

#155135

Purpose

Expresses N-terminally GFP- and HA-tagged E. coli GlpG from pGEX 4P-1

Depositor

Sinisa Urban

Article

Cho et al Nat Struct Mol Biol. 2019 Oct;26(10):910

Insert

GlpG (glpG Escherichia coli)

Tags

GST and HA

Expression

Bacterial

Promoter

tac

Available Since

Sept. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pYI001

Plasmid

#170833

Purpose

(GUSPlus::tOCS in pFP100)*

Depositor

Christian Fankhauser

Article

Ince et al Nat Commun. 2022 Oct 10;13(1):5659. doi:

Insert

GUSPlus::tOCS

Expression

Plant

Available Since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pC2xTC1-alphaTubulin

Plasmid

#36326

Depositor

Amy Palmer

Article

Van Engelenburg et al Chembiochem. 2010 Mar 1;11(4):489-93.

Insert

alpha tubulin 1b with tetracysteine peptides for ReAsH labeling (TUBA1B Synthetic, Human)

Tags

2xTC1

Expression

Mammalian

Promoter

CMV

Available Since

May 24, 2012

Availability

Academic Institutions and Nonprofits only

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pCMB-NUCr

Plasmid

#61169

Purpose

Fluorescent reporter for nucleus NLS-mCherry-GUS expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

NLS-mCherry-GUS

Tags

mCherry

Expression

Plant

Promoter

MtBCP1

Available Since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT/TO Plk4 KD-mCherry

Plasmid

#80270

Purpose

mammalian expression plasmid for kinase dead Plk4

Depositor

Andrew Holland

Article

Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

Insert

Plk4 (PLK4 Human)

Tags

mCherry

Expression

Mammalian

Mutation

D154A kinase dead

Promoter

CMV

Available Since

Aug. 10, 2016

Availability

Academic Institutions and Nonprofits only

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UBQ10:sXVE:(MCS)-3xHA-S11

Plasmid

#108180

Purpose

Binary vectors used for the inducible expression of tripartite split-sfGFP components (with a 3xHA tag) in plants

Depositor

Tzu-Yin Liu

Article

Liu et al Plant J. 2018 Feb 16. doi: 10.1111/tpj.1

Type

Empty backbone

Use

Synthetic Biology

Expression

Plant

Promoter

UBQ10:sXVE:

Available Since

April 13, 2018

Availability

Academic Institutions and Nonprofits only

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UBQ10:sXVE:S10-(MCS)-3xHA

Plasmid

#108178

Purpose

Binary vectors used for the inducible expression of tripartite split-sfGFP components (with a 3xHA tag) in plants

Depositor

Tzu-Yin Liu

Article

Liu et al Plant J. 2018 Feb 16. doi: 10.1111/tpj.1

Type

Empty backbone

Use

Synthetic Biology

Expression

Plant

Promoter

UBQ10:sXVE:

Available Since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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MSCV Puro-UBC:GFP

Plasmid

#68482

Purpose

Retrovirus expressing GFP driven by UBC promoter

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Inserts

Puromycin Resistance
GFP

Use

Retroviral

Expression

Mammalian

Promoter

LTR and UBC

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pFP.R364

Plasmid

#138234

Purpose

Expresses the split mCherry reporter interrupted by the Cth-Ter artificially mini-intein (in cis)

Depositor

Baojun Wang

Article

Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

Inserts

araC (araC )
mCherryN(NGK).Cth-Ter*.(SEL)mCherryC.H6

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Promoter

P(araBAD) and iPC

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pFP.R274

Plasmid

#138223

Purpose

Expresses the split mCherry reporter interrupted by the TvoVMA intein (in cis)

Depositor

Baojun Wang

Article

Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

Inserts

araC (araC )
mCherryN(GSK).TvoVMA.(TVI)mCherryC.H6

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Promoter

P(araBAD) and iPC

Available Since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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pGSTag Flag Jnk3a1

Plasmid

#15745

Depositor

Roger Davis

Article

Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

Insert

Jnk3a1 (MAPK10 Human)

Expression

Bacterial

Available Since

March 6, 2008

Availability

Academic Institutions and Nonprofits only