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  5. human SLC12A5-V5-HA-2A-Luciferase repair template

    Plasmid
    #158576
    Purpose
    For targeted insertion of V5-HA-2A-Luciferase reporter before the stop codon of the human SLC12A5 gene that encodes neuronal chloride transporter KCC2 protein.
    Depositor
    Rudolf Jaenisch
    Publication
    Tang et al Sci Transl Med. 2019 Jul 31;11(503). pii
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human SLC12A5 5' homology arm-V5-HA-2A-Luciferase-3' homology arm (SLC12A5 Human)
    Tags
    V5 and HA on the endogenous KCC2 protein product
    Expression
    Mammalian
    Available since
    Sept. 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    158576-plasmid-map-sequence-id-311917

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  17. pTSlb-Q758C

    Plasmid
    #60729
    Purpose
    Expresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD. C-terminal fragment has the point mutations Q758C
    Depositor
    Matthew Bennett
    Publication
    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
    Inserts
    Residues 1-179 of split T7 RNAP
    Residues 180-880 of split T7 RNAP
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    Residues 1-180 of split T7 RNAP and Residues 180-…
    Available since
    Nov. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    60729-plasmid-map-sequence-id-99392

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Showing: 7561 - 7580 of 8240 results