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pLEW100v5-PUR-VSG-228

Plasmid

#245106

Purpose

Inserts VSG-228 coding sequence in T. brucei rDNA spacer

Depositor

Monica Mugnier

Article

Smith et al Nature (2026)

Insert

VSG-228

Use

Other

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pLEW100v5-177-PUR-VSG-228

Plasmid

#245107

Purpose

Inserts VSG-228 coding sequence in T. brucei minichromosomes using the 177bp repeats

Depositor

Monica Mugnier

Article

Smith et al Nature (2026)

Insert

VSG-228

Use

Other

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pLEW100v5-PUR-VSG-228.noUTRS

Plasmid

#245109

Purpose

Inserts VSG-228 coding sequence without UTRs in T. brucei rDNA spacer

Depositor

Monica Mugnier

Article

Smith et al Nature (2026)

Insert

VSG-228

Use

Other

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pLEW100v5-PUR-VSG-228.500bp

Plasmid

#245110

Purpose

Inserts 500 bp of VSG-228 coding sequence in T. brucei rDNA spacer centered at AnTat1.1 position 694

Depositor

Monica Mugnier

Article

Smith et al Nature (2026)

Insert

VSG-228:500

Use

Other

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pLEW100v5-PUR-VSG-228.400bp

Plasmid

#245111

Purpose

Inserts 400 bp of VSG-228 coding sequence in T. brucei rDNA spacer centered at AnTat1.1 position 694

Depositor

Monica Mugnier

Article

Smith et al Nature (2026)

Insert

VSG-228:400

Use

Other

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pLEW100v5-PUR-VSG-228.300bp

Plasmid

#245112

Purpose

Inserts 300 bp of VSG-228 coding sequence in T. brucei rDNA spacer centered at AnTat1.1 position 694

Depositor

Monica Mugnier

Article

Smith et al Nature (2026)

Insert

VSG-228:300

Use

Other

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pLEW100v5-PUR-VSG-228.200bp

Plasmid

#245113

Purpose

Inserts 200 bp of VSG-228 coding sequence in T. brucei rDNA spacer centered at AnTat1.1 position 694

Depositor

Monica Mugnier

Article

Smith et al Nature (2026)

Insert

VSG-228:200

Use

Other

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pLEW100v5-PUR-VSG-228.100bp

Plasmid

#245114

Purpose

Inserts 100 bp of VSG-228 coding sequence in T. brucei rDNA spacer centered at AnTat1.1 position 694

Depositor

Monica Mugnier

Article

Smith et al Nature (2026)

Insert

VSG-228:100

Use

Other

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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tdTomato/pTREX-b

Plasmid

#68709

Purpose

Expresses tdTomato in pTREX-b vector which confers resistance to blasticidin. This vector is used for cloning a specific sgRNA by BamHI, to transfect Cas9-expressing Trypanosoma cruzi epimastigotes.

Depositor

Roberto Docampo

Article

Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.

Insert

tdTomato

Use

Expression of tdtomato red fluorescence protein i…

Available Since

Oct. 8, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pLEW100v5-PUR-VSG-228.100bp_offset

Plasmid

#245115

Purpose

Inserts 100 bp of VSG-228 coding sequence in T. brucei rDNA spacer overlapping AnTat1.1 position 694, centered around common VSG-228 insertion

Depositor

Monica Mugnier

Article

Smith et al Nature (2026)

Insert

VSG-228:100_offset

Use

Other

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge