We narrowed to 2,688 results for: GUS

1. pRK5-FLAG-KPTN

   Plasmid

   #87041

   Purpose

   mammalian expression of KPTN

   Depositor

   David Sabatini

   Article

   Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

   Insert

   KPTN (KPTN Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pRK5-FLAG-C12orf66

   Plasmid

   #87047

   Purpose

   mammalian expression of C12orf66

   Depositor

   David Sabatini

   Article

   Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

   Insert

   C12orf66 (C12orf66 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pRK5-FLAG-ITFG2

   Plasmid

   #87044

   Purpose

   mammalian expression of ITFG2

   Depositor

   David Sabatini

   Article

   Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

   Insert

   ITFG2 (ITFG2 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. FR_HNF4A2

   Plasmid

   #31100

   Depositor

   Gerhart Ryffel

   Article

   Thomas et al Nucleic Acids Res. 2004 . 32(19):e150.

   Insert

   HNF4A (HNF4A Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   splice variant 2

   Available Since

   Oct. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLenti-EF1a-Ngn3ER-PuroR-mCherry

   Plasmid

   #197385

   Purpose

   To establish Ngn3ER cell line for endocrine differentiation (4-hydroxytamoxifen activation, Puromycin resistant)

   Depositor

   Qiao Zhou

   Article

   Huang et al Nat Cell Biol. 2023 Apr 27. doi: 10.1038

   Insert

   Ngn3ER-2A-PuroR-2A-mCherry
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1A

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDarmo.CMVT_v1

   Plasmid

   #133072

   Purpose

   Entry vector for cloning genes under an enhanced CMVT promoter for expression in mammalian cells. Version 1.0.

   Depositor

   David Sabatini, Kacper Rogala

   Article

   Rogala et al Science. 2019 Oct 25;366(6464):468-475.

   Type

   Empty backbone

   Use

   Entry vector

   Tags

   3C-Flag-TwinStrep and TwinStrep-Flag-3C

   Expression

   Mammalian

   Promoter

   CMVT (Enhanced CMV)

   Available Since

   May 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLenti-TetO-PM

   Plasmid

   #197387

   Purpose

   To establish all-in-one TetO-inducible cell line for Pdx1-Mafa expression (Doxycycline induced; rtTA included; Blasticidin resistant)

   Depositor

   Qiao Zhou

   Article

   Huang et al Nat Cell Biol. 2023 Apr 27. doi: 10.1038

   Insert

   PDX1-T2A-MAFA
   

   Use

   Lentiviral; Teto

   Expression

   Mammalian

   Promoter

   TRE

   Available Since

   Feb. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. FLAG-Foxo1-WT 6KR

   Plasmid

   #17560

   Depositor

   Domenico Accili

   Article

   Kitamura et al Cell Metab. 2005 Sep . 2(3):153-63.

   Insert

   Foxo1 6KR (Foxo1 Mouse)
   

   Tags

   Flag and Myc

   Expression

   Mammalian

   Mutation

   K242, K245, K259, K262, K271 and K291 were replac…

   Promoter

   CMV

   Available Since

   Oct. 20, 2008

   Availability

   Academic Institutions and Nonprofits only

9. pX458-ECFP

   Plasmid

   #112220

   Purpose

   Cloning backbone for sgRNA, co-expresses human optimized S. pyogenes Cas9 and ECFP

   Depositor

   Yinming Liang

   Article

   Luo et al J Biotechnol. 2018 Jun 7;281:11-20. doi:

   Insert

   ECFP
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   July 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. FLAG-Foxo1ADA(pCMV5)

    Plasmid

    #12149

    Depositor

    Domenico Accili

    Article

    Kitamura et al Cell Metab. 2005 Sep . 2(3):153-63.

    Insert

    Foxo1 ADA (Foxo1 Mouse)
    

    Tags

    Flag and Myc

    Expression

    Mammalian

    Mutation

    Threonine 24 to Alanine (A) and Serine 253 to Asp…

    Promoter

    CMV

    Available Since

    June 13, 2007

    Availability

    Academic Institutions and Nonprofits only

11. GFP-itis pBE-t

    Plasmid

    #195342

    Purpose

    Base editor plasmid expressing ABE8e-NG-Cas9n under Theophylline Riboswitch and constituitively expressed gRNA targeting EGFP A110V

    Depositor

    Alexis Komor

    Article

    Vasquez et al CRISPR J. 2023 Apr 20. doi: 10.1089/cris

    Inserts

    ecTadA(8e)-SpCas9-NG
    gRNA ctctacgcgggtcttgtagt
    

    Use

    CRISPR

    Mutation

    SpCas9n-NG: D10A, L1111R, D1135V, G1218R, E1219F,…

    Promoter

    Lac and ProC

    Available Since

    Jan. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. GFP-itis pBE-nt

    Plasmid

    #195343

    Purpose

    Base editor plasmid expressing ABE8e-NG-Cas9n under Theophylline Riboswitch and constituitively expressed nonsense non-targeting gRNA

    Depositor

    Alexis Komor

    Article

    Vasquez et al CRISPR J. 2023 Apr 20. doi: 10.1089/cris

    Inserts

    ecTadA(8e)-SpCas9-NG
    gRNA gtgcacgacgccgtatgcga
    

    Use

    CRISPR

    Mutation

    SpCas9n-NG: D10A, L1111R, D1135V, G1218R, E1219F,…

    Promoter

    Lac and ProC

    Available Since

    Jan. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRK5-HA-C12orf66

    Plasmid

    #87048

    Purpose

    mammalian expression of C12orf66

    Depositor

    David Sabatini

    Article

    Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

    Insert

    C12orf66 (C12orf66 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pKLV-EF1amCherry-W

    Plasmid

    #159295

    Purpose

    Lentiviral vector expressing mCherry driven by human EF1a promoter

    Depositor

    Kosuke Yusa

    Article

    Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

    Insert

    mCherry
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    human EF1a promoter

    Available Since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pQE32-pr-mEosFP (mEosFP-V69T)

    Plasmid

    #99213

    Purpose

    Bacterial expression of the primed conversion capable protein pr-mEosFP (mEosFP-V69T).

    Depositor

    Periklis Pantazis

    Article

    Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

    Insert

    pr-mEosFP
    

    Expression

    Bacterial

    Mutation

    monomeric variant with mutation V69T for primed c…

    Promoter

    T5

    Available Since

    June 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRK5-HA-KPTN

    Plasmid

    #87042

    Purpose

    mammalian expression of KPTN

    Depositor

    David Sabatini

    Article

    Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

    Insert

    KPTN (KPTN Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. FLAG-Foxo1-WT 6KQ

    Plasmid

    #17562

    Depositor

    Domenico Accili

    Article

    Kitamura et al Cell Metab. 2005 Sep . 2(3):153-63.

    Insert

    Foxo1 6KQ (Foxo1 Mouse)
    

    Tags

    Flag and Myc

    Expression

    Mammalian

    Mutation

    K242, K245, K259, K262, K271 and K291 were replac…

    Promoter

    CMV

    Available Since

    Oct. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

18. pHH0103_NR1H2_Hormone-recep

    Plasmid

    #109827

    Purpose

    Protein expression and purification of NR1H2_Hormone-recep

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    NR1H2_Hormone-recep (NR1H2 Human)
    

    Expression

    Bacterial

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pdCASclos

    Plasmid

    #73640

    Purpose

    Transcriptional repression for gene Spo0A (CAC-2011) in Clostridium acetobutylicum ATCC 824

    Depositor

    Sheng Yang

    Article

    Li et al Biotechnol J. 2016 May 23. doi: 10.1002/

    Inserts

    dCas9
    sgRNA to spo0A
    

    Use

    E.coli-clostridium shuttle vector

    Mutation

    D10A, H840A

    Promoter

    Pj23119 and ptb

    Available Since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRK5-HA-ITFG2

    Plasmid

    #87045

    Purpose

    mammalian expression of ITFG2

    Depositor

    David Sabatini

    Article

    Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

    Insert

    ITFG2 (ITFG2 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge