We narrowed to 1,126 results for: gibson assembly

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pL0_AF-spacer

Plasmid

#202130

Purpose

Level 0 plasmid containing a small non-coding spacer sequence with AF overhangs used to build level 1 constructs.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

Spacer

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 25, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_AC-acc2

Plasmid

#202128

Purpose

Level 0 plasmid containing the promoter from gene 55209 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

acc2 promoter

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 25, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_AC-glna

Plasmid

#202127

Purpose

Level 0 plasmid containing the promoter from gene 22357 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

glna promoter

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 24, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_AC-dph1

Plasmid

#202126

Purpose

Level 0 plasmid containing the promoter from gene 54330 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

dph1 promoter

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 24, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_AC-NR

Plasmid

#202123

Purpose

Level 0 plasmid containing the promoter from gene 54983 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

NR promoter

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 24, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_AC-fcpB

Plasmid

#202121

Purpose

Level 0 plasmid containing the promoter from gene 25172 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

fcpB promoter

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 24, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_AC-vapol

Plasmid

#202118

Purpose

Level 0 plasmid containing the promoter from gene 47434 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

vapol promoter

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 24, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_AC-lysA

Plasmid

#202117

Purpose

Level 0 plasmid containing the promoter from gene 21592 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

lysA promoter

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 24, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_AC-HO

Plasmid

#202116

Purpose

Level 0 plasmid containing the promoter from gene 5902 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

HO promoter

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 24, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_AC-NRT

Plasmid

#202115

Purpose

Level 0 plasmid containing the promoter from gene 54101 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

NRT promoter

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 24, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_AC-sit4

Plasmid

#202114

Purpose

Level 0 plasmid containing the promoter from gene 48707 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

sit4 promoter

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 24, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_AC-act1

Plasmid

#202113

Purpose

Level 0 plasmid containing the promoter from gene 29812 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

act1 promoter

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 24, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_AC-CaMK2

Plasmid

#202112

Purpose

Level 0 plasmid containing the promoter from gene 39236 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

CaMK2 promoter

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 24, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_AC-fbaC5

Plasmid

#202111

Purpose

Level 0 plasmid containing the promoter from gene 41423 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

fbaC5 promoter

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 24, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_AB-OEE3

Plasmid

#202103

Purpose

Level 0 plasmid containing the promoter from gene 54499 from Phaeodactylum tricornutum with AB overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

OEE3 promoter

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_AB-P49

Plasmid

#202102

Purpose

Level 0 plasmid containing the promoter from gene 49202 from Phaeodactylum tricornutum with AB overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

P49 promoter

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_AB-NR

Plasmid

#202101

Purpose

Level 0 plasmid containing the promoter from gene 54983 from Phaeodactylum tricornutum with AB overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

NR promoter

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pL0_AB-H4

Plasmid

#202100

Purpose

Level 0 plasmid containing the promoter from gene 34971 from Phaeodactylum tricornutum with AB overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

H4 promoter

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pBN095

Plasmid

#173765

Purpose

express E. coli E69 (O9a:K30) Wzc with periplasmic motif 3 truncation

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc with periplasmic motif 3 truncation

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

delete motif 3

Promoter

araBAD

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBN215

Plasmid

#167962

Purpose

express E. coli E69 (O9a:K30) Wzc with Y715E, Y717E, Y718E

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc with Y715E, Y717E, Y718E

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

Y715E, Y717E, Y718E

Promoter

araBAD

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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