We narrowed to 10,574 results for: Kars

1. MSP469

   Plasmid

   #65771

   Purpose

   Human expression vector for SpCas9 VQR variant: CMV-T7-humanSpCas9(D1135V/R1335Q/T1337R)-NLS-3xFLAG (VQR variant)

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135V/R1335Q/T1337R)-NLS-3XFlag
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Mammalian

   Mutation

   D1135V, R1335Q, and T1337R mutations in Cas9

   Promoter

   CMV & T7

   Available since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTet-O-OVOL1-T2A-PuroR

   Plasmid

   #162347

   Purpose

   Lentiviral expression of OVOL1 under the control of the TetON promoter

   Depositor

   Charles Gersbach

   Article

   Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

   Insert

   OVOL1 (OVOL1 Human)
   

   Use

   Lentiviral

   Tags

   T2A-PuroR

   Expression

   Mammalian

   Mutation

   Promoter

   Tet-ON

   Available since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. CaTCH BC1

   Plasmid

   #154195

   Purpose

   Lentiviral CaTCH barcoding plasmid harboring a single barcode cassette (BC1)

   Depositor

   Anna Obenauf

   Article

   Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

   Insert

   CaTCH BC1
   

   Use

   Lentiviral; Catch barcode labeling

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFE-Rrub-FII-prk

   Plasmid

   #162695

   Purpose

   Expresses R. rubrum Form II rubisco and S. elongatus Prk

   Depositor

   David Savage

   Article

   Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

   Inserts

   Rubrum Form II rubisco
   S. elongatus PRK
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PLtet0-1 promoter and under same promoter

   Available since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. CaTCH_empty-backbone

   Plasmid

   #157746

   Purpose

   Lentiviral CaTCH barcoding plasmid harboring an emtpy cloning site to insert a CaTCH barcoding library or individual barcode sequences

   Depositor

   Anna Obenauf

   Article

   Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

   Type

   Empty backbone

   Use

   Lentiviral; Catch barcode cloning

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. MSP680

   Plasmid

   #65772

   Purpose

   Human expression vector for SpCas9 EQR variant: CMV-T7-humanSpCas9(D1135E/R1335Q/T1337R)-NLS-3xFLAG (EQR variant)

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135E/R1335Q/T1337R)-NLS-3XFlag
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Mammalian

   Mutation

   D1135E, R1335Q, and T1337R mutations in Cas9

   Promoter

   CMV & T7

   Available since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCSF107mT-GATEWAY-3'-Myc tag

   Plasmid

   #67617

   Purpose

   A pCS2 based vector to generate a 3'-Myc tag using GATEWAY cloning technology

   Depositor

   Todd Stukenberg

   Article

   Grant et al Dev Biol. 2015 Dec 15;408(2):345-57. doi

   Type

   Empty backbone

   Use

   In vitro transcription or translation off of an s…

   Tags

   C-terminal Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and SP6

   Available since

   Aug. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. MSP2292

   Plasmid

   #70707

   Purpose

   Bacterial expression plasmid for KKH SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG-T7-Sa-sgRNA(84) #2

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

   Inserts

   mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
   SaCas9 sgRNA (+84)
   

   Use

   CRISPR

   Tags

   NLS-3xFLAG

   Expression

   Bacterial

   Mutation

   E782K, N968K and R1015H in SaCas9

   Promoter

   T7

   Available since

   Dec. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. BPK2101

   Plasmid

   #65770

   Purpose

   Bacterial expression plasmid for S. aureus Cas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSaCas9-NLS-3xFLAG-T7-BsaIcassette-Sa-sgRNA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   mammalian codon-optimized SaCas9, and SaCas9 gRNA
   

   Use

   CRISPR

   Tags

   NLS-3xFlag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 (x2)

   Available since

   June 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. MEF2D-2A-neo

    Plasmid

    #190622

    Purpose

    Lentiviral expression plasmid of human MEF2D (long isoform), neomycin selection

    Depositor

    Junwei Shi

    Article

    Pingul et al iScience. 2022 Sep 15;25(10):105139. doi

    Insert

    MEF2D (MEF2D Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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