We narrowed to 23,735 results for: fus
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Plasmid#207407PurposeDonor template to knock in mCherry at the C-terminus of mouse ActB (β-Actin) using DSB repair by homology-mediated end joining (HMEJ). [Lab plasmid ID: TU148]DepositorAvailable SinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only
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pSN-A112C-1A32
Plasmid#191241PurposeExpresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-1A32 and a His-tag, for fluorescent studies.DepositorInsertnuc (nuclease A), HLA-A (HLAA) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
Tagsnuclease A from S. aureus fused to the poly-Leu T…ExpressionBacterialMutationChanged Alanine 112 to Cysteine in SN, and predic…PromoterT7 promoterAvailable SinceFeb. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSN-A112C-1A31
Plasmid#191242PurposeExpresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-1A31 and a His-tag, for fluorescent studies.DepositorInsertnuc (nuclease A), HLA-A (HLAA) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
Tagsnuclease A from S. aureus fused to the poly-Leu T…ExpressionBacterialMutationChanged Alanine 112 to Cysteine in SN, and predic…PromoterT7 promoterAvailable SinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSN-A112C-CP8B1
Plasmid#191240PurposeExpresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-CP8B1 and a His-tag, for fluorescent studies.DepositorInsertnuc (nuclease A), CYP8B1 (CYP12) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
Tagsnuclease A from S. aureus fused to the poly-Leu T…ExpressionBacterialMutationChanged Alanine 112 to Cysteine in SN, and predic…PromoterT7 promoterAvailable SinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1A-WWE-HA-nLuc-FLAG-T2A-WWE-myc-cLuc-FLAG-IRES-Puro
Plasmid#187611PurposeSplit luciferase construct with nLuc fused to C-terminus of WWE domain, linked by T2A to cLuc fused to C-terminus of WWE domain, linked by IRES to puromycin resistance cassetteDepositorInsertsWWE domain with C-terminal nLuc
WWE domain with C-terminal cLuc
UseLentiviralTagsFLAG, HA, Split luciferase (cLuc), Split lucifera…ExpressionMammalianPromoterEF1AAvailable SinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1A-WWE(R163A)-HA-nLuc-FLAG-T2A-WWE-myc-cLuc-FLAG-IRES-Puro
Plasmid#187612PurposeSplit luciferase construct with nLuc fused to C-terminus of WWE domain with Arg163Ala mutation, linked by T2A to cLuc fused to C-terminus of WWE domain, linked by IRES to puromycin resistance cassetteDepositorInsertsWWE domain (R163A) with C-terminal nLuc
WWE domain with C-terminal cLuc
UseLentiviralTagsFLAG, HA, Split luciferase (cLuc), Split lucifera…ExpressionMammalianMutationArg163AlaPromoterEF1AAvailable SinceSept. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCVD293
Plasmid#159765PurposeExpression vector for mutated Fc-fused ACE2(740)DepositorAvailable SinceMay 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
61427-sgHtt_10
Plasmid#157861PurposeExpresses a sgRNA targeting mouse Htt promoter for gene activation using the SAM systemDepositorAvailable SinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
61427-sgHtt_6
Plasmid#157860PurposeExpresses a sgRNA targeting mouse Htt promoter for gene activation using the SAM systemDepositorAvailable SinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2TKN-bp1
Plasmid#133428PurposeHuman BP1 fragment of SREBF1 in vector for bacterial expression of GST fusion protein.DepositorAvailable SinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
mOrange-imp α
Plasmid#119720PurposeExpresses mOrange-tagged human importinα in mammalian cellsDepositorInsertimportinα (KPNA2 Human)
TagsmOrangeExpressionMammalianMutationcontains amino acids 251-529PromoterCMVAvailable SinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZIP-hCMV-ZsGreen-P2A-Puro-IKZF1 sh1
Plasmid#123324PurposeLentiviral vector for expression of shRNA targeting IKZF1 from a miR30 embedded casette in the 3'UTR of ZsGreen-P2A-Puro insertDepositorInsertshRNA targeting IKZF1 embedded in miR30 backbone (IKZF1 Human)
UseLentiviralExpressionMammalianPromoterCMVAvailable SinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZIP-hCMV-ZsGreen-P2A-Puro-IKZF1 sh2
Plasmid#123325PurposeLentiviral vector for expression of shRNA targeting IKZF1 from a miR30 embedded casette in the 3'UTR of ZsGreen-P2A-Puro insertDepositorInsertshRNA targeting IKZF1 embedded in miR30 backbone (IKZF1 Human)
UseLentiviralExpressionMammalianPromoterCMVAvailable SinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSN29
Plasmid#100114Purposeexpresses a chimeric cMYC-repressor fusionDepositorUseLentiviralExpressionMammalianAvailable SinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSN17
Plasmid#100104Purposeexpresses a chimeric OCT4-repressor fusionDepositorUseLentiviralExpressionMammalianAvailable SinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFBD-Btk(PH)Akt1(KD)
Plasmid#86591PurposeExpresses chimeric fusion of PH domain of Btk and kinase domain of Akt1DepositorAvailable SinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
LAMTOR3 gRNA (BRDN0001147669)
Plasmid#77136Purpose3rd generation lentiviral gRNA plasmid targeting human LAMTOR3DepositorAvailable SinceJuly 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
CROT gRNA (BRDN0001145682)
Plasmid#77709Purpose3rd generation lentiviral gRNA plasmid targeting human CROTDepositorAvailable SinceJuly 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
KALRN gRNA (BRDN0001145005)
Plasmid#77639Purpose3rd generation lentiviral gRNA plasmid targeting human KALRNDepositorAvailable SinceJuly 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
CAMK2B gRNA (BRDN0001148102)
Plasmid#77742Purpose3rd generation lentiviral gRNA plasmid targeting human CAMK2BDepositorAvailable SinceJuly 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
AURKA gRNA (BRDN0001145351)
Plasmid#77728Purpose3rd generation lentiviral gRNA plasmid targeting human AURKADepositorAvailable SinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
CDK5R1 gRNA (BRDN0001145710)
Plasmid#77851Purpose3rd generation lentiviral gRNA plasmid targeting human CDK5R1DepositorAvailable SinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
C17orf75 gRNA (BRDN0001145175)
Plasmid#78077Purpose3rd generation lentiviral gRNA plasmid targeting human C17orf75DepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
C17orf75 gRNA (BRDN0001146368)
Plasmid#78078Purpose3rd generation lentiviral gRNA plasmid targeting human C17orf75DepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
C17orf75 gRNA (BRDN0001487133)
Plasmid#78079Purpose3rd generation lentiviral gRNA plasmid targeting human C17orf75DepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
LRGUK gRNA (BRDN0001145468)
Plasmid#78084Purpose3rd generation lentiviral gRNA plasmid targeting human LRGUKDepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
LRGUK gRNA (BRDN0001147114)
Plasmid#78085Purpose3rd generation lentiviral gRNA plasmid targeting human LRGUKDepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
LRGUK gRNA (BRDN0001487051)
Plasmid#78086Purpose3rd generation lentiviral gRNA plasmid targeting human LRGUKDepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
AURKC gRNA (BRDN0001146500)
Plasmid#78054Purpose3rd generation lentiviral gRNA plasmid targeting human AURKCDepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
AURKC gRNA (BRDN0001487140)
Plasmid#78055Purpose3rd generation lentiviral gRNA plasmid targeting human AURKCDepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only