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pMaCTag-Z25

Plasmid

#120068

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with YFP-LID

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

YFP-LID

Use

Pcr template

Tags

YFP-LID

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P32

Plasmid

#120043

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with S-Tag

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

S-Tag

Use

Pcr template

Tags

S-Tag

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCS_FLPER(T)

Plasmid

#31131

Depositor

Gerhart Ryffel

Article

Werdien et al Nucleic Acids Res. 2001 Jun 1. 29(11):E5

Insert

FLP-ER ligand binding domain (ESR1 Human, Budding Yeast)

Expression

Mammalian

Mutation

full length FLP linked to ligand binding domain o…

Available Since

Oct. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA5-hygro-eGFP-HEC1-9D

Plasmid

#114029

Purpose

Inducible expression of eGFP-tagged HEC1-9D in FLPin Trex cell lines

Depositor

Geert Kops

Article

Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

Insert

eGFP-HEC1 - 9D (NDC80) (NDC80 Human)

Expression

Mammalian

Mutation

Hec1 - 9D

Available Since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pFP.R375

Plasmid

#138245

Purpose

Expresses the split mCherry reporter interrupted by the NrdA-2 intein (in cis)

Depositor

Baojun Wang

Article

Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

Inserts

araC (araC )
mCherryN(SNL).NrdA-2*.(CNE)mCherryC.H6

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Promoter

P(araBAD) and iPC

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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CAV1-D67G-HA

Plasmid

#27706

Depositor

Ari Helenius

Article

Hayer et al Traffic. 2010 Mar . 11(3):361-82.

Insert

caveolin-1, mutant D67G (CAV1 Canis lupus familiaris)

Tags

HA

Expression

Mammalian

Mutation

mutant D67G

Available Since

July 28, 2011

Availability

Academic Institutions and Nonprofits only

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pCDNA3 T7 BimL

Plasmid

#24242

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

Bim L (BCL2L11 Human)

Tags

T7

Expression

Mammalian

Available Since

June 1, 2010

Availability

Academic Institutions and Nonprofits only
PIGAZFN-L2

Plasmid

#37798

Depositor

Linzhao Cheng

Article

Zou et al Cell Stem Cell. 2009 Jul 2. 5(1):97-110.

Insert

PIGA zinc finger L2

Expression

Mammalian

Promoter

CMV

Available Since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

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C455-E01: attB4-stuffer-attB5

Plasmid

#162983

Purpose

Gateway attB4-attB5 entry clone containing a non-transcribed/translated region for use in generating negative control vectors

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

stuffer fragment for vector controls

Use

Synthetic Biology

Available Since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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C515-E01: attB5r-stuffer-attB1r

Plasmid

#162968

Purpose

Gateway attB5r-attB1r entry clone containing a non-transcribed/translated region for use in generating negative control vectors

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

stuffer fragment for vector controls

Use

Synthetic Biology

Available Since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_Lyn11-STf

Plasmid

#182008

Purpose

CMV overexpression of SNAP-tag fast at the inner leaflet of the plasma membrane of mammalian cell lines

Depositor

Kai Johnsson

Article

Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

Insert

Lyn11-STf

Expression

Mammalian

Mutation

Fast mutation on SNAP-tag E30R

Promoter

CMV

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z03

Plasmid

#120046

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with moxCerulean3

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

moxCerulean3

Use

Pcr template

Tags

moxCerulean3

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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TAL2406

Plasmid

#36809

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

JAK2-TALEN_Left (Jak2 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
pSicoR-mCh-Chd1i1

Plasmid

#21904

Depositor

Miguel Ramalho-Santos

Article

Gaspar-Maia et al Nature. 2009 Aug 13;460(7257):863-8. doi

Insert

Chd1-i1

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Sept. 30, 2009

Availability

Academic Institutions and Nonprofits only

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pMA5.1 (mouse integrin alpha 5 probe)

Plasmid

#14097

Depositor

Richard Hynes

Article

Yang et al Development. 1993 Dec . 119(4):1093-105.

Insert

Mouse Alpha 5 Integrin (Itga5 Mouse)

Expression

Bacterial

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pEG BacMam N term myc eGFP 3C

Plasmid

#160682

Purpose

Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

Depositor

Eric Gouaux

Article

Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

Type

Empty backbone

Tags

myc eGFP 3C

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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TAL2237

Plasmid

#36688

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

CHD4-TALEN_Right (Chd4 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
pCDNA3.1-Muc2D3

Plasmid

#155216

Purpose

Expresses human Muc2 residues 858-1259

Depositor

Deborah Fass

Article

Javitt et al Cell. 2020 Oct 29;183(3):717-729.e16. do

Insert

Muc2 D3 (MUC2 Human)

Tags

6xHistadine

Expression

Mammalian

Available Since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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KG#470

Plasmid

#110930

Purpose

Expresses proteins pan-neuronally in C. elegans (entire nervous system)

Depositor

Kenneth Miller

Article

Schade et al Genetics. 2005 Feb;169(2):631-49. doi: 1

Inserts

rab-3 promoter
unc-54 3' control region

Expression

Bacterial

Available Since

June 11, 2018

Availability

Academic Institutions and Nonprofits only

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pET15b+PCNA1 WT

Plasmid

#66139

Purpose

Expresses the wild type of Sulfolobus solfataricus PCNA1

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

Insert

DNA polymerase sliding clamp 1

Tags

His6 tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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