We narrowed to 17,152 results for: URE

1. pJK538

   Plasmid

   #72445

   Purpose

   Produces Acetobacter aceti 1023 small hypothetical protein with N-terminal His6 tag (H6AaOrfX); N5-carboxyaminoimidazole ribonucleotide mutase (AaPurE1)

   Depositor

   T. J. Kappock

   Inserts

   hypothetical protein
   N5-carboxyaminoimidazole ribonucleotide mutase
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7 and n/a

   Available since

   Feb. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJK505

   Plasmid

   #71594

   Purpose

   Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe mutant (AaPurE1fww)

   Depositor

   T. J. Kappock

   Insert

   N5-carboxyaminoimidazole ribonucleotide mutase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   changes tryptophan-34 to phenylalanine, additiona…

   Promoter

   T7

   Available since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJK506

   Plasmid

   #71596

   Purpose

   Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp165>Phe mutant (AaPurE1wwf)

   Depositor

   T. J. Kappock

   Insert

   N5-carboxyaminoimidazole ribonucleotide mutase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   changes tryptophan-165 to phenylalanine, addition…

   Promoter

   T7

   Available since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSaCas9_GFP

   Plasmid

   #64709

   Purpose

   Co-expression of human codon-optimized Staphylococcus aureus Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells and other mammalian cells

   Depositor

   Kiran Musunuru

   Insert

   SaCas9-2A-GFP
   

   Use

   CRISPR

   Tags

   2A-GFP and 3xFLAG-SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. poRibo-T2

   Plasmid

   #69347

   Purpose

   Ribo-T with orthogonal anti-Shine-Dalgarno sequence, and pL G to T mutation

   Depositor

   Michael Jewett

   Article

   Orelle et al Nature. 2015 Jul 29. doi: 10.1038/nature

   Insert

   oRibo-T
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. SQ171fg/pRibo-T

   Plasmid

   #69343

   Purpose

   SQ171 fast growing strain supported by pRibo-T plasmid

   Depositor

   Michael Jewett

   Article

   Orelle et al Nature. 2015 Jul 29. doi: 10.1038/nature

   Insert

   Ribo-T
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pT7oGFP

   Plasmid

   #69346

   Purpose

   Orthogonal GFP message under T7 promoter

   Depositor

   Michael Jewett

   Article

   Orelle et al Nature. 2015 Jul 29. doi: 10.1038/nature

   Insert

   Super folder GFP
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSR1002

   Plasmid

   #208735

   Purpose

   Empty backbone containing the S. aureus LAC-p01 replicon

   Depositor

   Lauren Andrews

   Article

   Rondthaler et al ACS Synth Biol. 2023 Dec 8. doi: 10.1021

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJK626

   Plasmid

   #72461

   Purpose

   Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Cys/Tyr154>Phe mutant (AaPurE1-S57C,Y154F)

   Depositor

   T. J. Kappock

   Insert

   N5-carboxyaminoimidazole ribonucleotide mutase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Mutation changes serine-57 to cysteine, tyrosine-…

   Promoter

   T7

   Available since

   March 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJK292

    Plasmid

    #73371

    Purpose

    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, His59>Asn/His89>Asn mutant (AaPurE1-H59N,H89N)

    Depositor

    T. J. Kappock

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changed histidine-59 to asparagine and histidine-…

    Promoter

    T7

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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