Addgene

We narrowed to 2,362 results for: GCG

Showing: 761 - 780 of 2362 results

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pWT029c

Plasmid

#96858

Purpose

guanine-agRNA (GFP activation)

Depositor

David Liu

Article

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

guanine-agRNA (GFP activation)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pDD426

Plasmid

#202079

Purpose

Frame +1/-0 selector for NHEJ knock-in

Depositor

Daniel Dickinson

Article

Shi et al Development. 2023 May 15;150(10):dev2013

Insert

pEF1a-mCherry-Cas9 + Frame +1/-0 sgRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

EF1a

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pDD425

Plasmid

#202078

Purpose

Frame +0 selector for NHEJ knock-in

Depositor

Daniel Dickinson

Article

Shi et al Development. 2023 May 15;150(10):dev2013

Insert

pEF1a-mCherry-Cas9 + Frame +0 sgRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

EF1a

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pDD427

Plasmid

#202080

Purpose

Frame +2/-1 selector for NHEJ knock-in

Depositor

Daniel Dickinson

Article

Shi et al Development. 2023 May 15;150(10):dev2013

Insert

pEF1a-mCherry-Cas9 + Frame +2/-1 sgRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

EF1a

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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miniCMV-(mNeonGreen)4-tDeg

Plasmid

#129402

Purpose

(mNeonGreen)4-tDeg fluorogenic protein

Depositor

Samie Jaffrey

Article

Wu et al Nat Methods. 2019 Sep;16(9):862-865. doi

Insert

(mNeonGreen)-tDeg

Use

Tags

Expression

Mammalian

Mutation

Promoter

miniCMV (GGTAGGCGTGTACGGTGGGAGGCCTATATAAGCAGAGCT)

Available since

Aug. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pTAKN2_J23119-sgRNA

Plasmid

#108552

Purpose

Bacterial sgRNA expression vector

Depositor

Akihiko Kondo

Article

Banno et al Nat Microbiol. 2018 Feb 5. pii: 10.1038/

Insert

2x Streptococcus pyogenes sgRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

J23119

Available since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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ZO-1 shRNA 3253

Plasmid

#37206

Depositor

Alan Fanning, James Anderson

Article

Van Itallie et al Mol Biol Cell. 2009 Sep;20(17):3930-40.

Insert

dog ZO-1 shRNA (TJP1 canine)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 27, 2012

Availability

Academic Institutions and Nonprofits only
GEARBOCS-SPARCL1 KO

Plasmid

#218182

Purpose

To KO Hevin in astrocytes

Depositor

Cagla Eroglu

Article

Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

Insert

sgRNA (Sparcl1 Mouse)

Use

AAV and CRISPR

Tags

Expression

Mutation

Promoter

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pTK73.ChrV_oxTi365.gRNA

Plasmid

#194052

Purpose

sgRNA targeting ChrV_oxTi365 locus of the C. elegans genome

Depositor

Kota Mizumoto

Article

Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

Insert

ChrV_oxTi365 sgRNA

Use

Tags

Expression

Worm

Mutation

Promoter

U6

Available since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR BFP-guide1

Plasmid

#118157

Purpose

CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA1 against the BFP CDS

Depositor

Jorge Ferrer

Insert

KRAB-dCas9-P2A-BlastR

Use

CRISPR and Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

EF1a core and U6

Available since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pBT009.J1.J306

Plasmid

#177325

Purpose

scRNA targeting J3 promoter for activation

Depositor

James Carothers

Article

Tickman et al Cell Syst. 2021 Nov 17. pii: S2405-4712(

Insert

J306

Use

Tags

Expression

Mutation

Promoter

Available since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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MS2-pegRNA

Plasmid

#181811

Purpose

ms2 incorporated pegRNA-1.1

Depositor

Erik Sontheimer

Article

Liu et al Nat Biotechnol. 2022 Apr 4. pii: 10.1038

Insert

MS2-pegRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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GESTALT_pX330-v1

Plasmid

#103061

Purpose

plasmid px330 with guide targeting GESTALT v1 through V5 constructs

Depositor

Jay Shendure

Article

McKenna et al Science. 2016 May 26. pii: aaf7907.

Insert

integration of Cas9 with guide targeting GESTALT barcodes V1 to V5

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pBbS5c-AcrB/AcrD/AcrFa, ABO_0964

Plasmid

#86206

Purpose

Expresses efflux pump in E. coli to provide resistance to biofuel (low copy)

Depositor

Mary Dunlop

Article

Turner et al ACS Synth Biol. 2015 Oct 16;4(10):1056-6

Insert

Efflux pump

Use

Tags

Expression

Bacterial

Mutation

Promoter

ATCGTTTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATAA…

Available since

March 2, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti-Crispr-V2-HMID.v1

Plasmid

#103062

Purpose

LentiCrisprV2 with guide targeting V1-V5 GESTALT barcodes

Depositor

Jay Shendure

Article

McKenna et al Science. 2016 May 26. pii: aaf7907.

Insert

Cas9 plus expressed GESTALT V1-V5 guide

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pMM100

Plasmid

#127225

Purpose

AtU6::sgRNA targeting PDS

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

sgRNA

Use

Tags

Expression

Plant

Mutation

codon optimized CDSs

Promoter

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pSLQ1371_sgNanog

Plasmid

#63710

Purpose

Express sgNanog in mammalian cells of mice.

Depositor

Stanley Qi

Article

Yu et al Cell Stem Cell. 2015 Feb 5;16(2):142-7.

Insert

sgNanog, CMV promoter, puro, mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 27, 2015

Availability

Academic Institutions and Nonprofits only

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LRT2B-GO

Plasmid

#136908

Purpose

Lentivirus expressing GO sgRNA

Depositor

Lukas Dow

Article

Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

Insert

sgGO

Use

Lentiviral

Tags

Expression

Mutation

WT

Promoter

hU6

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pX330.LoxP

Plasmid

#66586

Purpose

sgRNA for LSL

Depositor

Wen Xue

Article

Li et al Genome Biol. 2015 May 28;16(1):111.

Insert

LoxP sgRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only
pX330.iGFP2

Plasmid

#66582

Purpose

sgRNA for iGFP

Depositor

Wen Xue

Article

Li et al Genome Biol. 2015 May 28;16(1):111.

Insert

iGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 27, 2015

Availability

Academic Institutions and Nonprofits only