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pU6-sgRNA EF1Alpha-puro-T2A-BFP.Control

Plasmid

#128749

Purpose

Expresses control gRNA

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

Control sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mU6

Available since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pLY101-RetroEFS-HER2-28BBz-PRODH2-WPRE

Plasmid

#192202

Purpose

Retro-HER2CAR-PRODH2

Depositor

Sidi Chen

Article

Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

Insert

Retro-HER2CAR-PRODH2 (ERBB2 Synthetic)

Use

Retroviral; Mammalian expression

Tags

Expression

Mutation

NA

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Intron-Tagging-mScarlet-Frame1-Parental-Minicircle

Plasmid

#216125

Purpose

Parental minicircle containing mScarlet-I flanked by a splice acceptor and splice donor. Used with other intron tagging plasmids for placing the mScarlet tag as a synthetic exon into frame 1 introns of target genes.

Depositor

Stefan Kubicek

Article

Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

Insert

sgRNA-SA-(GGGGS)x4-mScarlet-I-(GGGGS)x4-SD

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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CycK-GFP (1-270AA) in Cilantro (Codon optimized)

Plasmid

#169930

Purpose

Overexpression of 1-270AA CycK-GFP fusion

Depositor

Benjamin Ebert

Article

Slabicki et al Nature. 2020 Sep;585(7824):293-297. doi:

Insert

CCNK (CCNK Human)

Use

Lentiviral

Tags

GFP

Expression

Mutation

Promoter

Available since

July 28, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Ras-LOCKR-S R89L (negative control)

Plasmid

#216497

Purpose

FRET-based reporter for Ras activity, untargeted, negative control

Depositor

David Baker

Article

Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

Insert

Ras-LOCKR-S R89L negative control

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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shHIF2α(#2) (HIF2α-#3-pSUPER-retro-GFP/Neo)

Plasmid

#22132

Depositor

William Kaelin

Article

Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

Insert

synthethic oligonucleotide

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

synthethic oligonucleotide encoding shRNA targeti…

Promoter

Available since

Feb. 1, 2010

Availability

Academic Institutions and Nonprofits only

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pRetroX-Tight-puro-3xFLAG-RUNX3 R122C

Plasmid

#203439

Purpose

Mammalian expression of RUNX4

Depositor

Yoshiaki Ito

Article

Oei et al Commun Biol. 2023 Jul 3;6(1):689. doi: 1

Insert

RUNX3 (RUNX3 Human)

Use

Tags

3 x FLAG

Expression

Mammalian

Mutation

R122C

Promoter

Available since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pET-28a-GFP-Patronin CC 868–1457

Plasmid

#59053

Purpose

for E. coli expression of Patronin CC truncation

Depositor

Ron Vale

Article

Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

Insert

Patronin (Patronin Fly)

Use

Tags

His, Strep, TEV, and sfGFP

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 2, 2014

Availability

Academic Institutions and Nonprofits only
pSUPER-retro-puro-tetO-CTR1-shRNA

Plasmid

#53180

Purpose

Expresses shRNA against human CTR1 from puromycin resistance retroviral vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

CTR1 (SLC31A1 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 19, 2014

Availability

Academic Institutions and Nonprofits only
pEYFPC1-ECFP/GgVcl 1-419 control 1 FRET

Plasmid

#46277

Depositor

Susan Craig

Article

Chen et al J Cell Biol. 2005 May 9;169(3):459-70.

Insert

ECFP + Vcl 1-419 (VCL Chicken)

Use

Tags

EYFP

Expression

Mammalian

Mutation

aa 1-419

Promoter

CMV

Available since

Aug. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pJEP212-Lenti-1.3CaMKII-P-Intron-MCS-WPRE-pA

Plasmid

#62931

Purpose

pLenti vector backbone containing a 1.3CaMKII promoter followed by an intron followed by multiple cloning site(MCS) (reverse orientation).

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

1.3αCaMKII

Available since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pLN410 (FuGW-S(E2F1)p-ECFP sponge control)

Plasmid

#105182

Purpose

Module 2 control - synthetic promoter E2F1 drives ECFP only (no miR1 binding sites)

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

S(E2F1)p-ECFP (Cerulean) sponge control

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 17, 2018

Availability

Academic Institutions and Nonprofits only

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nCas9-UGI control for Target-AID (pRZ1047)

Plasmid

#131299

Purpose

CAG promoter expression plasmid for NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-NLS-UGI-P2A-EGFP (Target-AID without pmCDA1).

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

Insert

NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-NLS-UGI-P2A-EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pSUPER-retro-puro-tetO-Mek1-shRNA

Plasmid

#53179

Purpose

Expresses shRNA against mouse Mek1 from puromycin resistance retroviral vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

MEK1 (Map2k1 Mouse)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 19, 2014

Availability

Academic Institutions and Nonprofits only
pET-21a-GFP-Patronin CC 1288–1457

Plasmid

#59055

Purpose

for E. coli expression of Patronin CC truncation

Depositor

Ron Vale

Article

Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

Insert

Patronin (Patronin Fly)

Use

Tags

GFP

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn INTRON HaloTag-STIM1 WPRE

Plasmid

#236233

Purpose

AAV expression of mouse STIM1 internally tagged with HaloTag

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

HaloTag-Stim1 (Stim1 Mouse)

Use

AAV

Tags

HaloTag

Expression

Mutation

Promoter

human Synapsin 1

Available since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn INTRON HaloTag-Sec61b WPRE

Plasmid

#236229

Purpose

AAV expression of a marker for the endoplasmic reticulum, Sec61b, fused to HaloTag

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

HaloTag-Sec61b (SEC61B Human, Synthetic)

Use

AAV

Tags

HaloTag

Expression

Mutation

Promoter

human Synapsin 1

Available since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn INTRON mCherry-Jph3 WPRE

Plasmid

#236237

Purpose

AAV expression of human Junctophilin3 N-terminally tagged with mCherry

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

Junctophilin 3 (JPH3 Human)

Use

AAV

Tags

mCherry

Expression

Mutation

Promoter

human Synapsin 1

Available since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn INTRON mTagBFP2-CAAX2 WPRE

Plasmid

#236231

Purpose

AAV expression of a fluorescent marker, mTagBFP2, fused to the plasma membrane targeting sequence CAAX2

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

mTagBFP2 fused to the plasma membrane targeting sequence CAAX2

Use

AAV

Tags

mTagBFP2

Expression

Mutation

Promoter

human Synapsin 1

Available since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn INTRON mScarlet-STIM2 WPRE

Plasmid

#236236

Purpose

AAV expression of human STIM2 internally tagged with mScarlet-I

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

mScarletI-STIM2 (STIM2 Human)

Use

AAV

Tags

mScarletI in position 29

Expression

Mutation

Signal peptide from STIM1 and residues 15-746 of …

Promoter

human Synapsin 1

Available since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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