We narrowed to 159,258 results for: addgene

1. XLone-Puro SPI1 P2A mCherry

   Plasmid

   #140029

   Purpose

   Tunable and temporal expression control of SPI1 and mCherry

   Depositor

   Xiaoping Bao

   Insert

   SPI1 (SPI1 Human)
   

   Use

   Synthetic Biology

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   May 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. 4QQD

   Plasmid

   #61947

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   UHRF1:SDC130-H02:C39674 (UHRF1 Human)
   

   Use

   Tags

   6xHis and MHHHHHHSSGRENLYFQG

   Expression

   Bacterial

   Mutation

   See comments

   Promoter

   T7

   Available since

   March 2, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

3. pLV V5-lamin B1

   Plasmid

   #175108

   Purpose

   Lentiviral expression of V5-tagged mouse Lmnb1

   Depositor

   Larry Gerace

   Insert

   Lmnb1 (Lmnb1 Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTF277 (pFA6a-TEV-6xGly-2xStrep-KanMX)

   Plasmid

   #44094

   Depositor

   Tim Formosa

   Type

   Empty backbone

   Use

   Pcr-based yeast c-terminal tagging

   Tags

   2xStrep tag, 6xGly linker, ADH1 transcription ter…

   Expression

   Mutation

   Promoter

   Available since

   April 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-CMV-FLEX-SaCas9-U6-sgVamp2

   Plasmid

   #159920

   Purpose

   Mutagenesis of Vamp2

   Depositor

   Larry Zweifel

   Insert

   Vamp2 (Vamp2 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. MSCV-mMDM2-IRES-GFP

   Plasmid

   #66954

   Purpose

   retroviral construct for expression of mouse Mdm2 cloned into the EcoRI site

   Depositor

   Martine Roussel

   Insert

   Mdm2 (Mdm2 Mouse)
   

   Use

   Retroviral

   Tags

   IRES-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   MSCV

   Available since

   July 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTF279 (pFA6a-TEV-6xGly-2xStrep-HphMX)

   Plasmid

   #44082

   Depositor

   Tim Formosa

   Type

   Empty backbone

   Use

   Pcr-based yeast c-terminal tagging

   Tags

   2xStrep tag, 6xGly linker, ADH1 transcription ter…

   Expression

   Mutation

   Promoter

   Available since

   April 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLBS.EF/mScarlet(i)-hCdt100Nt

   Plasmid

   #129334

   Purpose

   Cell cycle reporter, lentivirus

   Depositor

   Yasushi Okada

   Article

   Yaginuma et al bioRxiv 2021.10.08.463131

   Insert

   mScarlet(i) (Addgene 85044)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV CMV FST-FLAG

   Plasmid

   #175167

   Purpose

   Lentiviral expression of human FST-FLAG

   Depositor

   Larry Gerace

   Insert

   FST (FST Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. TPH2

    Plasmid

    #73243

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    TPH2 (TPH2 Human)
    

    Use

    Tags

    His-6-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. 8VIS

    Plasmid

    #220881

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    TMPRSS11D (TMPRSS11D Human)
    

    Use

    Baculovirus expresssion

    Tags

    EFVEHHHHHHHH

    Expression

    Mutation

    Promoter

    Polyhedrin

    Available since

    Sept. 30, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. TBC1D7

    Plasmid

    #32047

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    TBC1D7 (TBC1D7 Human)
    

    Use

    Tags

    His (with TEV cleavage)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 3, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

13. 5VBD (USP09X)

    Plasmid

    #101260

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    USP09X (USP9X Human)
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 2, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pZC13 (pFA6a-TEV-6xGly-V5-HphMX)

    Plasmid

    #44085

    Depositor

    Tim Formosa

    Type

    Empty backbone

    Use

    Pcr-based yeast c-terminal tagging

    Tags

    6xGly linker, ADH1 transcription terminator, HphM…

    Expression

    Mutation

    Promoter

    Available since

    April 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBabe H-Ras

    Plasmid

    #18744

    Depositor

    Scott Lowe

    Insert

    H-Ras (HRAS Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    G12V

    Promoter

    Available since

    July 18, 2008

    Availability

    Academic Institutions and Nonprofits only

16. pLV Agpat2-V5

    Plasmid

    #175159

    Purpose

    Lentiviral expression of mouse Agpat2-V5

    Depositor

    Larry Gerace

    Insert

    Agpat2 (Agpat2 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. PANK1

    Plasmid

    #32871

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PANK1 (PANK1 Human)
    

    Use

    Tags

    His tag (with Thrombin cleavage)

    Expression

    Bacterial

    Mutation

    amino acids 231-597 only

    Promoter

    Available since

    Jan. 6, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

18. UHRF1

    Plasmid

    #28163

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    UHRF1 (UHRF1 Human)
    

    Use

    Tags

    His tag with TEV cleavage

    Expression

    Bacterial

    Mutation

    tandem tudor domain

    Promoter

    Available since

    Feb. 28, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

19. pUltra-hot-H2B-mTagBFP2

    Plasmid

    #161792

    Purpose

    3rd generation lentiviral multicistronic vector for mammalian expression of mCherry, H2B-mTagBFP2, and one gene of interest

    Depositor

    Jorge Luis Arias-Arias

    Insert

    H2B-mTagBFP2
    

    Use

    Lentiviral; Multicistronic

    Tags

    mCherry and mTagBFP2

    Expression

    Mammalian

    Mutation

    I174A in the mTagBFP2 protein according to PLoS O…

    Promoter

    UbC

    Available since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLV Cnep1r1-V5

    Plasmid

    #175133

    Purpose

    Lentiviral expression of mouse Cnep1r1-V5

    Depositor

    Larry Gerace

    Insert

    Cnep1r1 (Cnep1r1 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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