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RAD21 C-tag CRISPR

Plasmid

#140540

Purpose

RAD21 tagging CRISPR

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

RAD21 (RAD21 Human)

Use

CRISPR

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV/mIba1.GFP.WPRE.miR-9.T.miR-129-2-3p.T.SV40pA

Plasmid

#190163

Purpose

Microglia-targeted transgene expression.

Depositor

Hirokazu Hirai

Article

Okada et al Commun Biol. 2022 Nov 11;5(1):1224. doi:

Inserts

mIba1 promoter, 1.7-kb, microglia-specific promoter
miR-9-Tx4, 4 repeated miRNA target sites for miR-9
miR-129-2-3p-Tx4, 4 repeated miRNA target sites for miR-129-2-3p

Use

AAV

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-GRAB_rDA1m

Plasmid

#140556

Purpose

Expresses the red fluorescent medium-affinity DA sensor GRAB_rDA1m in neurons

Depositor

Yulong Li

Article

Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.

Has Service

AAV9

Insert

Red fluorescent DA sensor GRAB_rDA1m (medium affinity)

Use

AAV

Promoter

hSyn

Available Since

July 29, 2020

Availability

Academic Institutions and Nonprofits only

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pHR-TetOn-mCherry-OAZ1_FS-YFP

Plasmid

#232356

Purpose

Mammalian expression of dox-inducible polyamine sensor (polyamine levels = YFP/Cherry)

Depositor

Ankur Jain

Article

Sharma et al bioRxiv [Preprint]. 2024 Nov 17:2024.08.

Insert

OAZ1 derived polyamine sensing module (OAZ1 Human)

Use

Lentiviral

Tags

eYFP and mCherry

Expression

Mammalian

Promoter

TRE3G

Available Since

March 7, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti CMV TRE3G Puro FLAG-DD-ER-mCherry-TRF1-FokI WT

Plasmid

#207832

Purpose

telomere specific nuclease to create double-strand breaks at telomeres

Depositor

Roger Greenberg

Article

Dilley et al Nature. 2016 Nov 3;539(7627):54-58. doi:

Insert

TRF1 (TERF1 Human)

Use

Lentiviral

Tags

Flag, DD, ER, mCherry and FokI

Expression

Mammalian

Promoter

TRE3G

Available Since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti CMV TRE3G Puro FLAG-DD-ER-mCherry-TRF1-FokI D450A

Plasmid

#207953

Purpose

nuclease inactive control for TRF1FokI WT

Depositor

Roger Greenberg

Article

Dilley et al Nature. 2016 Nov 3;539(7627):54-58. doi:

Insert

TRF1 (TERF1 Human)

Use

Lentiviral

Tags

Flag, DD, ER, mCherry and FokI (D450A)

Expression

Mammalian

Promoter

TRE3G

Available Since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-EF1A-LivePAR-Hygro

Plasmid

#176063

Purpose

EGFP fused to the C-terminus of a WWE domain & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

LivePAR

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1A

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3/F-tractin=mStayGold

Plasmid

#212019

Purpose

For filamentous actin labeling. Alternatively, the F-tractin gene can be replaced with a target molecule gene for C-terminal tagging with mStayGold through a linker [(GGGGS)3] (denoted as ‘=’).

Depositor

Atsushi Miyawaki

Article

Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

F-tractin

Tags

mStayGold

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-TetO-hNGN2-Puro

Plasmid

#79049

Purpose

Expresses human NEUROGENIN2 (hNGN2) and puromycin resistance gene under control of TetON promoter. This lentiviral vector is used to generate NGN2-iNs (induced neurons) from hiPSCs and hiPSC-NPCs.

Depositor

Kristen Brennand

Article

Ho et al Methods. 2015 Nov 25. pii: S1046-2023(15

Insert

hNGN2-P2A-PuroR (NEUROG2 Human)

Use

Lentiviral

Promoter

TetO

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-rtTA

Plasmid

#175274

Purpose

AAV vector mediating neuron-specific expression of rtTA

Depositor

Wei Xu

Article

Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

Has Service

AAV1

Insert

Reverse tetracycline-controlled transactivator (rtTA)

Use

AAV

Promoter

Synapsin

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pLSB-KAN

Plasmid

#166700

Purpose

Combined sgRNA/Cas9 pLSB vector with kanMX6 (G418) marker. Golden Gate-ready for cloning custom sgRNA sequences.

Depositor

Robin Allshire

Article

Torres-Garcia et al Wellcome Open Res. 2020 Nov 24;5:274. do

Inserts

tRNA promoter : sgRNA cassette
adh15 promoter : Cas9 codon-optimised for S. pombe

Expression

Yeast

Promoter

adh15 promoter and tRNA Ser promoter

Available Since

April 28, 2021

Availability

Academic Institutions and Nonprofits only

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pDisCoTune

Plasmid

#173713

Purpose

Promotes expression of disulfide-rich protein in E. coli T7 expression system. The plasmid encodes expression of the folding factors Erv1p and hPDI controlled by the Ptac promoter.

Depositor

Morten Norholm

Article

Bertelsen et al Microb Biotechnol. 2021 Nov;14(6):2566-2

Inserts

ERV1 (ERV1 Budding Yeast)
PDI

Expression

Bacterial

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-LC3

Plasmid

#21073

Purpose

Mammalian expression of rat LC3 fused to EGFP

Depositor

Tamotsu Yoshimori

Article

Kabeya et al EMBO J. 2000 Nov 1. 19(21):5720-8.

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Tags

EGFP

Expression

Mammalian

Mutation

First ATG is not included.

Promoter

CMV

Available Since

May 11, 2009

Availability

Academic Institutions and Nonprofits only

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pET-Hia5-OPT1

Plasmid

#231968

Purpose

Expresses DNA adenine N6-methyltransferase Hia5 in Escherichia coli (E.coli) T7 Express lysY/Iq

Depositor

Schahram Akbarian

Article

Peter et al Cell Rep Methods. 2024 Nov 25:100911. do

Insert

DNA adenine N6-methyltransferase Hia5

Tags

Histidine tag

Expression

Bacterial

Available Since

Sept. 4, 2025

Availability

Academic Institutions and Nonprofits only

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HaloTag-LaminA

Plasmid

#231894

Purpose

Expression of HaloTag-tagged LaminA to visualize the nuclear lamina

Depositor

Lukas Kapitein

Article

Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

Insert

LaminA (LMNA Human)

Tags

HaloTag

Expression

Mammalian

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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PG13-luc (wt p53 binding sites)

Plasmid

#16442

Purpose

Luciferase reporter containing 13 copies of the p53-binding consensus sequence

Depositor

Bert Vogelstein

Article

el-Deiry et al Cell. 1993 Nov 19. 75(4):817-25.

Insert

p53-binding site (TP53 )

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

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(attB) tdMCP-tagged BRaf with MS2-circRNA

Plasmid

#226163

Purpose

Used to integrate 3xFlag-tdMCP-BRaf and MS2-circRNA barcodes into RNA-Protein Landing Pad cell lines

Depositor

Dustin Maly

Article

Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

Inserts

3xFlag-tdMCP-BRaf (BRAF Human)
IRES-mCherry-P2A-PuroR
MS2-circRNA barcode

Use

Bxb1 attb, no promoter

Tags

3xFlag-tdMCP

Expression

Mammalian

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1/Halo-CAAX

Plasmid

#249173

Purpose

Mammalian expression vector of CAAX motif (plasma membrane marker) fused with Halo-tag

Depositor

Pakorn Kanchanawong

Article

Nishimura et al J Cell Sci. 2025 Nov 24:jcs.264110. doi:

Insert

CAAX (HRAS Human)

Tags

Halo

Expression

Mammalian

Mutation

Only 10 amino acids ending with CVLS (CAAX motif)

Promoter

CMV

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.2/V5-DEST RBM14-V5

Plasmid

#69823

Purpose

Expresses RBM14-V5 in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

RNA-binding protein 14 (RBM14 Human)

Use

Retroviral

Tags

v5

Expression

Mammalian

Promoter

cmv

Available Since

April 25, 2016

Availability

Academic Institutions and Nonprofits only

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pUltraI-Tet3.0[TAA]

Plasmid

#164580

Purpose

Machinery plasmid containing a single copy of the Mb Tet3.0RS and cognate tRNA for incorporation of Tet3.0 at TAA codons

Depositor

Ryan Mehl

Article

Bednar et al ACS Chem Biol. 2021 Nov 19;16(11):2612-2

Inserts

Mb Tet3.0RS (R2-3)
Mm tRNA[TAA]

Expression

Bacterial

Mutation

L170G, N311G, C313A

Promoter

ProK and TacI

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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