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pAc5.1B-lambdaN-HA-DmTral-S13AI15A_C

Plasmid

#146081

Purpose

Insect Expression of DmTral-S13AI15A

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmTral-S13AI15A (tral Fly)

Expression

Insect

Mutation

three silent mutations A198C, A387G, A1437G and 4…

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmTral_394-543-V5His6_D

Plasmid

#146134

Purpose

Insect Expression of DmTral_394-543

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmTral_394-543 (tral Fly)

Expression

Insect

Mutation

two silent mutations (nucleotides A198C, A387G) a…

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmTral-LSm-R21EE23KR42EF44A-V5His6_C

Plasmid

#146071

Purpose

Insect Expression of DmTral-LSmR21EE23KR42EF44A

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmTral-LSmR21EE23KR42EF44A (tral Fly)

Expression

Insect

Mutation

R21E, E23K, R42E, F44A. Additionally, one silent …

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmTral-R42EF44A_C

Plasmid

#146061

Purpose

Insect Expression of DmTral-R42EF44A

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmTral-R42EF44A (tral Fly)

Expression

Insect

Mutation

R42E, F44A. Additionally, two silent mutations A1…

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmTral-LSm-R21EE23KS13AI15A-V5His6_C

Plasmid

#146049

Purpose

Insect Expression of DmTral-LSmR21EE23KS13AI15A

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmTral-LSmR21EE23KS13AI15A (tral Fly)

Expression

Insect

Mutation

S13A, I15A, R21E, E23K, one silent mutation A198C…

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmTral-FDF-ADA_C

Plasmid

#146039

Purpose

Insect Expression of DmTral-FDF-ADA

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

Insert

DmTral-FDF-ADA (tral Fly)

Expression

Insect

Mutation

FDF to ADA (407-409) mutation. Additionally, thre…

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pU6-chiRNA_dCrkgRNA1

Plasmid

#131139

Purpose

Encodes gRNA targeting Drosophila crk locus. Cuts 110bp upstream of the transcription start site. gRNA cloned into BbsI site of pU6-BbsI-chiRNA (Gratz et al. Genetics. 2013).

Depositor

Mark Peifer

Article

Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

Insert

dCrk gRNA1 (Crk Fly)

Use

CRISPR

Expression

Insect

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pU6-chiRNA_dCrkgRNA2

Plasmid

#131140

Purpose

Encodes gRNA targeting Drosophila crk locus. Cuts 99bp beyond the 3' UTR. gRNA cloned into BbsI site of pU6-BbsI-chiRNA (Gratz et al. Genetics. 2013).

Depositor

Mark Peifer

Article

Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

Insert

dCrk gRNA1 (Crk Fly)

Use

CRISPR

Expression

Insect

Available Since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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mei-S332-GFP 5.6kb genomic DNA in Casper4 S123-125-3A

Plasmid

#112987

Purpose

In P element vector for transposon insertion of mei-S332-GFP 5.6kb genomic DNA (S123-125-3A) into Drosophila genome

Depositor

Terry Orr-Weaver

Article

Nogueira et al G3 (Bethesda). 2014 Jul 31;4(10):1849-58

Insert

mei-S332 (mei-S332 Fly)

Use

P insertion vector

Tags

GFP

Mutation

S123-125-3A

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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mei-S332-GFP 5.6kb genomic DNA in Casper4 S123-125-3D

Plasmid

#112985

Purpose

In P element vector for transposon insertion of mei-S332-GFP 5.6kb genomic DNA (S123-125-3D) into Drosophila genome

Depositor

Terry Orr-Weaver

Article

Nogueira et al G3 (Bethesda). 2014 Jul 31;4(10):1849-58

Insert

mei-S332 (mei-S332 Fly)

Use

P insertion vector

Tags

GFP

Mutation

S123-125-3D

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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