We narrowed to 5,623 results for: crispr cas9 grna plasmid

1. eSpCas9(1.1)_No_FLAG_ATP1A1-G7

   Plasmid

   #173203

   Purpose

   Expresses the ATP1A1 G7 sgRNA in combination with FLAGless eSpCas9(1.1) to target ATP1A1 intron 17. pX330-like plasmid.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1 G7 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter

   Available since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pgRNAtet-phaZ

   Plasmid

   #169874

   Purpose

   Single guide RNA plasmid for Cas9 targeting phaZ in P. putida

   Depositor

   Brian Pfleger

   Article

   Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

   Insert

   sgRNA towards phaZ in Pseudomonas putida
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pgRNAkan-glpR

   Plasmid

   #169873

   Purpose

   Single guide RNA plasmid for Cas9 targeting glpR in P. putida

   Depositor

   Brian Pfleger

   Article

   Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

   Insert

   sgRNA towards glpR in Pseudomonas putida
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pU6-sgRosa26-1_CBh-Cas9-T2A-BFP-P2A-Ad4E1B

   Plasmid

   #64219

   Purpose

   Expression vector for a sgRNA against the mouse Rosa26 locus and Cas9 linked via T2A to BFP linked to the Ad4 E1B55K gene via P2A

   Depositor

   Ralf Kuehn

   Article

   Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

   Inserts

   Cas9
   sgRNA targeting ROSA26-1
   

   Use

   CRISPR

   Tags

   3xFLAG, NLS, and T2A-BFP-P2A-E1B

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and U6

   Available since

   May 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. B-HypaR-SpCas9

   Plasmid

   #126764

   Purpose

   Expression plasmid for human codon-opt. increased fidelity Blackjack-Hypa2SpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than Hypa2SpCas9.

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-Hypa2SpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   R661A; N692A; M694A; Q695A; H698A; amino acids 10…

   Promoter

   CBh

   Available since

   Sept. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSimpleII-U6-tracr-U6-crRNA(tdTomato)-NLS-NmCas9-HA-NLS(s)

   Plasmid

   #47869

   Purpose

   All-in-one plasmid targeting tdTomato

   Depositor

   Erik Sontheimer, James Thomson

   Article

   Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.

   Inserts

   NmCas9
   U6pr-tracrRNA
   tdTomato crRNA
   

   Use

   CRISPR

   Tags

   HA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCRISPRyl

   Plasmid

   #70007

   Purpose

   CRISPR/Cas9 vector for Yarrowia lipolytica, with AvrII site for sgRNA insertion

   Depositor

   Ian Wheeldon

   Article

   Schwartz et al ACS Synth Biol. 2015 Dec 29.

   Inserts

   Codon optimized Cas9 from S. pyogenes
   sgRNA expression cassette
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   SCR1'-tRNA and UAS1B8-TEF(136)

   Available since

   Feb. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. HypaR-SpCas9

   Plasmid

   #126757

   Purpose

   Expression plasmid for human codon-optimized increased fidelity Hypa2SpCas9 (without U6-sgRNA coding sequence, with silent mutations)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   Hypa2SpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   R661A, N692A, M694A, Q695A, H698A

   Promoter

   CBh

   Available since

   Sept. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pIW447-CRISPR XYL2

   Plasmid

   #98906

   Purpose

   K. marxianus CRISPR Plasmid for XYL2 editing

   Depositor

   Ian Wheeldon

   Article

   Lobs et al Biotechnol Biofuels. 2017 Jun 24;10:164.

   Inserts

   Codon optimized Cas9
   sgRNA expression cassette
   

   Use

   CRISPR and Synthetic Biology

   Tags

   SV40

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. Nme2Cas9_AAV

    Plasmid

    #119924

    Purpose

    All-in-one AAV plasmid expressing Nme2Cas9 with sgRNA cassette

    Depositor

    Erik Sontheimer

    Article

    Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

    Insert

    Nme2Cas9
    

    Use

    AAV

    Tags

    NLS-3xHA-NLS and NLS-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    U1a

    Available since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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