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pWT016e

Plasmid

#96851

Purpose

dHHR-bsgRNA-GFP (10 nt blocker)

Depositor

David Liu

Article

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

dHHR-bsgRNA-GFP (10 nt blocker)

Expression

Mammalian

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pWT016a

Plasmid

#96852

Purpose

HHR-bsgRNA-GFP (13 nt blocker)

Depositor

David Liu

Article

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

HHR-bsgRNA-GFP (13 nt blocker)

Expression

Mammalian

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pWT016b

Plasmid

#96853

Purpose

dHHR-bsgRNA-GFP (13 nt blocker)

Depositor

David Liu

Article

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

dHHR-bsgRNA-GFP (13 nt blocker)

Expression

Mammalian

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pMyo-3::oxCerulean-oxVenus::Lgg-1

Plasmid

#68044

Purpose

Lgg-1 tagged with oxCerulean-oxVenus (dFP, or double fluorescent protein), expressed in body wall muscle, for monitoring autophagy

Depositor

Dana Miller

Article

Chapin et al Aging (Albany NY). 2015 Jun 30.

Insert

pMyo-3::oxCerulean-oxVenus::Lgg-1

Expression

Worm

Available Since

Aug. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pCVD630

Plasmid

#66163

Purpose

cloning of ace gene

Depositor

James Kaper

Article

Trucksis et al Proc Natl Acad Sci U S A. 1993 Jun 1;90(

Insert

ace

Use

Cloning vector

Promoter

Unknown

Available Since

June 18, 2015

Availability

Academic Institutions and Nonprofits only
pBad_crtEBI_25

Plasmid

#63805

Purpose

expresses crtEBI genes for neurosporene production

Depositor

Howard Salis

Article

Farasat et al Mol Syst Biol. 2014 Jun 21;10:731. doi:

Insert

crtEBI operon

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

PBAD

Available Since

May 15, 2015

Availability

Academic Institutions and Nonprofits only

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pBad_crtEBI_14

Plasmid

#63804

Purpose

expresses crtEBI genes for neurosporene production

Depositor

Howard Salis

Article

Farasat et al Mol Syst Biol. 2014 Jun 21;10:731. doi:

Insert

crtEBI operon

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

PBAD

Available Since

May 7, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pBad_crtEBI_64

Plasmid

#63809

Purpose

expresses crtEBI genes for neurosporene production

Depositor

Howard Salis

Article

Farasat et al Mol Syst Biol. 2014 Jun 21;10:731. doi:

Insert

crtEBI operon

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

PBAD

Available Since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
LacI_PlacO1_crtEBI_13

Plasmid

#63812

Purpose

expresses crtEBI genes for neurosporene production

Depositor

Howard Salis

Article

Farasat et al Mol Syst Biol. 2014 Jun 21;10:731. doi:

Insert

crtEBI operon

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

Placo1

Available Since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pBad_crtEBI_70

Plasmid

#63810

Purpose

expresses crtEBI genes for neurosporene production

Depositor

Howard Salis

Article

Farasat et al Mol Syst Biol. 2014 Jun 21;10:731. doi:

Insert

crtEBI operon

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

PBAD

Available Since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pAD03 S. bayanus telomerase RNA KBS

Plasmid

#61892

Purpose

Plasmid for run-off in vitro transcription of Saccharomyces bayanus KBS RNA

Depositor

Thomas Cech

Article

Dalby et al RNA. 2013 Jun;19(6):841-51. doi: 10.1261

Insert

Telomerase RNA hairpin

Promoter

T7

Available Since

March 13, 2015

Availability

Academic Institutions and Nonprofits only
pAD05 S. castellii telomerase RNA KBS

Plasmid

#61894

Purpose

Plasmid for run-off in vitro transcription of Saccharomyces castellii KBS RNA

Depositor

Thomas Cech

Article

Dalby et al RNA. 2013 Jun;19(6):841-51. doi: 10.1261

Insert

Telomerase RNA hairpin

Promoter

T7

Available Since

March 13, 2015

Availability

Academic Institutions and Nonprofits only
pAD02 S. paradoxus telomerase RNA KBS

Plasmid

#61891

Purpose

Plasmid for run-off in vitro transcription of Saccharomyces paradoxus KBS RNA

Depositor

Thomas Cech

Article

Dalby et al RNA. 2013 Jun;19(6):841-51. doi: 10.1261

Insert

Telomerase RNA hairpin

Promoter

T7

Available Since

March 13, 2015

Availability

Academic Institutions and Nonprofits only
pAD04 S. arboricola telomerase RNA KBS

Plasmid

#61893

Purpose

Plasmid for run-off in vitro transcription of Saccharomyces arboricola KBS RNA

Depositor

Thomas Cech

Article

Dalby et al RNA. 2013 Jun;19(6):841-51. doi: 10.1261

Insert

Telomerase RNA hairpin

Promoter

T7

Available Since

March 13, 2015

Availability

Academic Institutions and Nonprofits only
pET-22HT-MSI1(7-192)Q30D

Plasmid

#60346

Purpose

Q30D mutant of the mouse MSI1 RBD

Depositor

Sean Ryder

Article

Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

Insert

mouse MSI1 (7-192) (Msi1 Mouse)

Tags

His and TEV protease site

Expression

Bacterial

Mutation

Q30D; contains amino acids 7-192 of Msi1

Promoter

T7

Available Since

Dec. 1, 2014

Availability

Academic Institutions and Nonprofits only
pET-22HT-MSI1(7-192)R53E/R61E

Plasmid

#60354

Purpose

R53E R61E double mutant of the mouse MSI1 RBD

Depositor

Sean Ryder

Article

Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

Insert

mouse MSI1 (7-192) (Msi1 Mouse)

Tags

His and TEV protease site

Expression

Bacterial

Mutation

R53E and R61E mutations; contains amino acids 7-1…

Promoter

T7

Available Since

Nov. 6, 2014

Availability

Academic Institutions and Nonprofits only
pET-22HT-MSI1(7-192)R53E

Plasmid

#60352

Purpose

R53E mutant of the mouse MSI1 RBD

Depositor

Sean Ryder

Article

Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

Insert

mouse MSI1 (7-192) (Msi1 Mouse)

Tags

His and TEV protease site

Expression

Bacterial

Mutation

R53E; contains amino acids 7-192 of Msi1

Promoter

T7

Available Since

Nov. 6, 2014

Availability

Academic Institutions and Nonprofits only
pET-22HT-MSI1(7-192)G64A

Plasmid

#60351

Purpose

G64A mutant of the mouse MSI1 RBD

Depositor

Sean Ryder

Article

Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

Insert

mouse MSI1 (7-192) (Msi1 Mouse)

Tags

His and TEV protease site

Expression

Bacterial

Mutation

G64A; contains amino acids 7-192 of Msi1

Promoter

T7

Available Since

Nov. 6, 2014

Availability

Academic Institutions and Nonprofits only
pET-22HT-MSI1(7-192)H83L

Plasmid

#60350

Purpose

H83L mutant of the mouse MSI1 RBD

Depositor

Sean Ryder

Article

Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

Insert

mouse MSI1 (7-192) (Msi1 Mouse)

Tags

His and TEV protease site

Expression

Bacterial

Mutation

H83L; contains amino acids 7-192 of Msi1

Promoter

T7

Available Since

Nov. 6, 2014

Availability

Academic Institutions and Nonprofits only
pET-22HT-MSI1(7-192)G35D

Plasmid

#60347

Purpose

G35D mutant of the mouse MSI1 RBD

Depositor

Sean Ryder

Article

Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

Insert

mouse MSI1 (7-192) (Msi1 Mouse)

Tags

His and TEV protease site

Expression

Bacterial

Mutation

G35D; contains amino acids 7-192 of Msi1

Promoter

T7

Available Since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only