Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 243 results for: trypanosoma

Showing: 61 - 80 of 243 results

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SpIMPDH

Plasmid

#206980

Purpose

Streptococcus pyogenes Inosine 5′-Monophosphate Dehydrogenase (SpIMPDH, EC. 1.1.1.205). Contains His6 tag with TEV protease cleavage site in pET-28b(+) plasmid for protein expression.

Depositor

Vern Schramm

Article

Glockzin et al Biochemistry. 2022 Oct 4;61(19):2088-210

Insert

SpIMPDH (guaB Streptococcus pyogenes)

Tags

His tag (with TEV cleavage site)

Expression

Bacterial

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pGL2084

Plasmid

#137726

Purpose

Gateway vector for inducible stem loop RNAi in T.b. brucei 2T1

Depositor

Jeremy Mottram

Article

Jones et al PLoS Pathog. 2014 Jan;10(1):e1003886. do

Type

Empty backbone

Use

RNAi; Trypanosoma brucei tet inducible stem loop

Available Since

June 20, 2023

Availability

Academic Institutions and Nonprofits only

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pRPa-c6MYCn

Plasmid

#69242

Purpose

Tet-inducible RRNA promoter driven plasmid for expression of N or C-terminally c-Myc tagged protein from a specific RRNA spacer locus in T. brucei.

Depositor

David Horn

Article

Alsford et al Mol Biochem Parasitol. 2008 Sep;161(1):7

Type

Empty backbone

Use

Expression in trypanosoma brucei

Tags

cMyc

Promoter

RRNA promoter

Available Since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pLEW100v5-NEO-T7-sgRNA

Plasmid

#245118

Purpose

Plasmid backbone to for inserting a T7 promoter driven guide RNA into the rDNA spacer of T. brucei

Depositor

Monica Mugnier

Article

Smith et al Nature. 2026 Apr 8. doi: 10.1038/s41586-

Type

Empty backbone

Use

CRISPR

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pLEW100v5-BSD-FLAG-La-Cas9

Plasmid

#245102

Purpose

Inserts tetracycline-inducible codon optimized, nuclceus targeted FLAG-Cas9 into T. brucei cells at the rDNA spacer

Depositor

Monica Mugnier

Article

Smith et al Nature. 2026 Apr 8. doi: 10.1038/s41586-

Insert

3xFLAG-La-Cas9

Use

CRISPR

Tags

3xFLAG-La

Mutation

codon optimized for T. brucei

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pLEW100v5-PUR-T7-sgRNA

Plasmid

#245108

Purpose

Plasmid backbone to for inserting a T7 promoter driven guide RNA into the rDNA spacer of T. brucei

Depositor

Monica Mugnier

Article

Smith et al Nature. 2026 Apr 8. doi: 10.1038/s41586-

Type

Empty backbone

Use

CRISPR

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pLEW100v5-BSD-VSG-228

Plasmid

#245116

Purpose

Inserts VSG-228 coding sequence in T. brucei rDNA spacer

Depositor

Monica Mugnier

Article

Smith et al Nature. 2026 Apr 8. doi: 10.1038/s41586-

Insert

VSG-228

Use

Other

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pLEW100v5-NEO-VSG-228

Plasmid

#245117

Purpose

Inserts VSG-228 coding sequence in T. brucei rDNA spacer

Depositor

Monica Mugnier

Article

Smith et al Nature. 2026 Apr 8. doi: 10.1038/s41586-

Insert

VSG-228

Use

Other

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19-HYG-BES1-AnTat1.1-telo

Plasmid

#245103

Purpose

Inserts VSG AnTat1.1 in T. brucei cells at Lister427 BES1

Depositor

Monica Mugnier

Article

Smith et al Nature. 2026 Apr 8. doi: 10.1038/s41586-

Insert

AnTat1.1

Use

Other

Mutation

silent G to A mutation at position 471 within AnT…

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19-HYG-BES1-VSG-228-telo

Plasmid

#245104

Purpose

Inserts VSG-228 in T. brucei cells at Lister427 BES1

Depositor

Monica Mugnier

Article

Smith et al Nature. 2026 Apr 8. doi: 10.1038/s41586-

Insert

VSG-228

Use

Other

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pHD309-PUR-VSG-228

Plasmid

#245105

Purpose

Inserts VSG-228 coding sequence in T. brucei tubulin array

Depositor

Monica Mugnier

Article

Smith et al Nature. 2026 Apr 8. doi: 10.1038/s41586-

Insert

VSG-228

Use

Other

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pLEW100v5-PUR-VSG-228

Plasmid

#245106

Purpose

Inserts VSG-228 coding sequence in T. brucei rDNA spacer

Depositor

Monica Mugnier

Article

Smith et al Nature. 2026 Apr 8. doi: 10.1038/s41586-

Insert

VSG-228

Use

Other

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pRP-c6MYCn

Plasmid

#69243

Purpose

Tet-inducible RRNA promoter driven plasmid for inducible expression of N- or C-terminally c-Myc tagged protein from a random RRNA spacer locus in T. brucei.

Depositor

David Horn

Article

Alsford et al Mol Biochem Parasitol. 2008 Sep;161(1):7

Type

Empty backbone

Use

Expression in trypanosoma brucei

Tags

cMyc

Promoter

RRNA promoter

Available Since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pLEW100v5-177-PUR-VSG-228

Plasmid

#245107

Purpose

Inserts VSG-228 coding sequence in T. brucei minichromosomes using the 177bp repeats

Depositor

Monica Mugnier

Article

Smith et al Nature. 2026 Apr 8. doi: 10.1038/s41586-

Insert

VSG-228

Use

Other

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pLEW100v5-PUR-VSG-228.noUTRS

Plasmid

#245109

Purpose

Inserts VSG-228 coding sequence without UTRs in T. brucei rDNA spacer

Depositor

Monica Mugnier

Article

Smith et al Nature. 2026 Apr 8. doi: 10.1038/s41586-

Insert

VSG-228

Use

Other

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pLEW100v5-BSD-T7-sgRNA

Plasmid

#245119

Purpose

Plasmid backbone to for inserting a T7 promoter driven guide RNA into the rDNA spacer of T. brucei

Depositor

Monica Mugnier

Article

Smith et al Nature. 2026 Apr 8. doi: 10.1038/s41586-

Type

Empty backbone

Use

CRISPR

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pLEW100v5-PUR-VSG-228.500bp

Plasmid

#245110

Purpose

Inserts 500 bp of VSG-228 coding sequence in T. brucei rDNA spacer centered at AnTat1.1 position 694

Depositor

Monica Mugnier

Article

Smith et al Nature. 2026 Apr 8. doi: 10.1038/s41586-

Insert

VSG-228:500

Use

Other

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pLEW100v5-PUR-VSG-228.400bp

Plasmid

#245111

Purpose

Inserts 400 bp of VSG-228 coding sequence in T. brucei rDNA spacer centered at AnTat1.1 position 694

Depositor

Monica Mugnier

Article

Smith et al Nature. 2026 Apr 8. doi: 10.1038/s41586-

Insert

VSG-228:400

Use

Other

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pLEW100v5-PUR-VSG-228.300bp

Plasmid

#245112

Purpose

Inserts 300 bp of VSG-228 coding sequence in T. brucei rDNA spacer centered at AnTat1.1 position 694

Depositor

Monica Mugnier

Article

Smith et al Nature. 2026 Apr 8. doi: 10.1038/s41586-

Insert

VSG-228:300

Use

Other

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pLEW100v5-PUR-VSG-228.200bp

Plasmid

#245113

Purpose

Inserts 200 bp of VSG-228 coding sequence in T. brucei rDNA spacer centered at AnTat1.1 position 694

Depositor

Monica Mugnier

Article

Smith et al Nature. 2026 Apr 8. doi: 10.1038/s41586-

Insert

VSG-228:200

Use

Other

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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Showing: 61 - 80 of 243 results

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