We narrowed to 1,058 results for: gibson assembly

1. pLV_3xFLAG-ATP6V0A3

   Plasmid

   #228866

   Purpose

   Stable expression of V-ATPase subunit ATP6V0A3 with N-terminal 3xFLAG

   Depositor

   Wilhelm Palm

   Article

   Ratto et al Nat Commun. 2022 Aug 17;13(1):4848. doi:

   Insert

   ATP6V0A3 (TCIRG1 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   Available since

   Nov. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pEVOL-NBK-1

   Plasmid

   #207639

   Purpose

   A plasmid for the expression of M. mazei pyrrolysine aminoacyl-tRNA synthetase/tRNA pair (PylRS/tRNAPyl) in E. Coli.

   Depositor

   Cole DeForest

   Article

   Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

   Insert

   Pyl-tRNACUA and 2x pyrrolysyl-tRNA synthetase (MmPylRS)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   The evolved synthetase NBK-1 contains mutations Y…

   Promoter

   araBAD

   Available since

   Oct. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMMK ENTR 2

   Plasmid

   #206261

   Purpose

   ENTR Vector 1 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Empty backbone.

   Depositor

   Imre Berger

   Article

   Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

   Type

   Empty backbone

   Use

   Multimate/gateway entr 2

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMMK ENTR 1

   Plasmid

   #206260

   Purpose

   ENTR Vector 1 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Empty backbone.

   Depositor

   Imre Berger

   Article

   Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

   Type

   Empty backbone

   Use

   Multimate/gateway entr 1

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMMK ENTR 3

   Plasmid

   #206262

   Purpose

   ENTR Vector 1 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Empty backbone.

   Depositor

   Imre Berger

   Article

   Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

   Type

   Empty backbone

   Use

   Multimate/gateway entr 3

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMMK ENTR 4

   Plasmid

   #206263

   Purpose

   ENTR Vector 1 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Empty backbone.

   Depositor

   Imre Berger

   Article

   Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

   Type

   Empty backbone

   Use

   Multimate/gateway entr 4

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. TRE-mClover3

   Plasmid

   #214922

   Purpose

   expression vector control - contitutive expression of mClover3 alone

   Depositor

   Ophir Shalem

   Article

   Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d

   Insert

   mClover3
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. TRE-mRuby3

   Plasmid

   #214923

   Purpose

   expression vector control - contitutive expression of mRuby33 alone

   Depositor

   Ophir Shalem

   Article

   Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d

   Insert

   mRuby3
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. R26TV LSL-TRL

   Plasmid

   #68476

   Purpose

   Rosa26 targeting vector expressing Cre-dependent tTR-KRAB-rtTA3-Luc cassette

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Insert

   tTR-KRAB-2A-rtTA3-2A-Luciferase
   

   Use

   Cre/Lox, Luciferase, and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. TOPO UBC

    Plasmid

    #68456

    Purpose

    TOPO construct expressing UBC for generation of GMAP-compatible promoter

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Insert

    Ubiquitin C Promoter (UBC Human)
    

    Use

    Gmap

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Plac

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pPW3763 : CMVd3-HsIRE1dLKR-HaloTag

    Plasmid

    #185685

    Purpose

    Low-level transient expression of HsIRE1 lacking the linker, kinase, and RNase domains, with a C-terminal HaloTag.

    Depositor

    Peter Walter

    Article

    Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

    Insert

    ERN1 (ERN1 Synthetic, Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Deletion of linker, kinase, and Rnase domains

    Promoter

    Available since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV-hSyn-mCherry-T2A-miniSOG-C-Far

    Plasmid

    #193915

    Purpose

    Expresses miniSOG targeted to the plasma membrane of neurons, joined by a T2A cleavage site with mCherry; for AAV production

    Depositor

    Xiao Wang

    Article

    Sessler et al Sci Adv. 2022 Dec 9;8(49):eade1136. doi:

    Insert

    miniSOG
    

    Use

    AAV

    Tags

    Farnesylation signal sequence and Flag tag

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA5/FRT/TO - RBM28 E5 WT minigene

    Plasmid

    #169269

    Purpose

    Mammalian vector for constitutive expression of RBM28 Exon 5 WT minigene

    Depositor

    Susan Baserga

    Article

    Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

    Insert

    RBM28 E5 WT minigene (RBM28 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. 6xHis-TEV-GEI-17(133-509)

    Plasmid

    #87056

    Purpose

    Expresses 6xHis-tagged gei-17 (isoform f), with a TEV protease cleavage site between the tag and the ORF

    Depositor

    Ronald Hay

    Article

    Pelisch et al Nat Commun. 2014 Dec 5;5:5485. doi: 10.1

    Insert

    GEI-17 isfoomr f (aa 133-509) (gei-17 Nematode)
    

    Use

    Tags

    6xHis-TEV and GAGGGS linker

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHS1742

    Plasmid

    #239838

    Purpose

    OrufIscB-KRK mammalian expression

    Depositor

    Feng Zhang

    Article

    Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038

    Insert

    OrufIscB-KRK
    

    Use

    Tags

    Nucleoplasmin NLS-3xHA and SV40 NLS

    Expression

    Mammalian

    Mutation

    Insertion of REC domain from NbaCas9-1 + E137K/E4…

    Promoter

    CMV

    Available since

    July 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHS2096

    Plasmid

    #239835

    Purpose

    NovaIscB E. coli expression for recombinant purification

    Depositor

    Feng Zhang

    Article

    Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038

    Insert

    NovaIscB
    

    Use

    Tags

    14xHis-TwinStrep-bdSUMO

    Expression

    Bacterial

    Mutation

    Insertion of REC domain from NbaCas9-1 and swap o…

    Promoter

    T7

    Available since

    July 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pnCS_mEGFP-DSep2_Peanut-Strep

    Plasmid

    #174496

    Purpose

    bacterial co-expression of Drosophila Sep2 fused to monomeric EGFP and of Drosophila Peanut

    Depositor

    Manos Mavrakis

    Article

    Mavrakis et al Methods Cell Biol. 2016;136:199-220. doi

    Inserts

    Sep2 (Sep2 Fly)
    peanut (pnut Fly)
    

    Use

    Tags

    Strep and mEGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. deltaVP1 + VP2C-MIOX

    Plasmid

    #166680

    Purpose

    Yeast integrative plasmid for expressing deltaVP1 (GAL1 promoter) and mouse myo-inositol oxygenase tagged with VP2C (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

    Inserts

    VP2C-MIOX
    Murine polyomavirus deltaVP1 (NLS deletion mutant)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1 and GAL10

    Available since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p414GPD-3xFLAG-Nop4 dE5

    Plasmid

    #169263

    Purpose

    S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-Nop4 delta Exon 5

    Depositor

    Susan Baserga

    Article

    Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

    Insert

    Nop4 delta E5 (NOP4 Budding Yeast)
    

    Use

    Tags

    3xFLAG

    Expression

    Yeast

    Mutation

    deleted region corresponding to human Exon 5

    Promoter

    GPD

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p414GPD-3xFLAG-Nop4 dE8

    Plasmid

    #169264

    Purpose

    S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-Nop4 delta Exon 8

    Depositor

    Susan Baserga

    Article

    Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

    Insert

    Nop4 delta E8 (NOP4 Budding Yeast)
    

    Use

    Tags

    3xFLAG

    Expression

    Yeast

    Mutation

    deleted region corresponding to human Exon 8

    Promoter

    GPD

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge